Methods of phycobiliprotein extraction from gracilaria crassa and its applications in food colourants

Algal Research

Volumn 8, Issue , 2015, Pages 115-120

  Sudhakar, M P ^a   Jagatheesan, A ^b   Perumal, K ^a   Arunkumar, K ^b  

^a Shri AMM Murugappa Chettiar Research Centre   (India)

^b ALAGAPPA GOVERNMENT ARTS COLLEGE   (India)

Author keywords

Diethylaminoethyl cellulose 52; Filtration; Gracilaria crassa; Phycobiliproteins; Preservatives; Stability; Time correlated single photon counting spectrometer

Indexed keywords

GRACILARIA CRASSA;

EID: 84924761249     PISSN: 22119264     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.algal.2015.01.011     Document Type: Article

References (32)

1. Apt K.E., Collier J.L., Grossman A.R. Evolution of the phycobiliproteins. J. Mol. Biol. 1995, 248:79-96.
2. Kumar M., Kumari P., Gupta V., Reddy C.R.K., Jha B. Biochemical responses of red alga Gracilaria corticata (Gracilariales, Rhodophyta) to salinity induced oxidative stress. J. Exp. Mar. Biol. Ecol. 2010, 391:27-34.
3. Baghel R.S., Reddy C.R.K., Jha B. Characterization of agarophytic seaweeds from the biorefinery context. Bioresour. Technol. 2014, 159:280-285.
4. Boobathy S., Soundarapandian P., Prithivraj M., Gunasundari V. Biochemical characterization of protein isolated from seaweed, Gracilaria edulis. Curr. Res. J. Biol. Sci. 2010, 2:35-37.
5. Wang G. Isolation and purification of phycoerythrin from red alga Gracilaria verrucosa by Expanded-Bed-Adsorption and Ion-Exchange Chromatography. Chromatographia 2002, 56:509-513.
6. Rowan K.S. Photosynthetic Pigments of Algae 1989, 334. Cambridge University Press, New York.
7. Isailovic D., Li H.W., Yeung E.S. Isolation and characterization of R-phycoerythrin subunits and enzymatic digests. J. Chromatogr. A 2004, 1051:119-130.
8. Arad S.M., Yaron A. Natural pigments from red microalgae for use in foods and cosmetics. Trends Food Sci. Technol. 1992, 3:92-97.
9. Bermejo R., Talavera E.M., Alvarez-Pez J.M. Chromatographic purification and characterization of B-phycoerythrin from Porphyridium cruentum semipreparative high-performance liquid chromatographic separation and characterization of its subunits. J. Chromatogr. A 2001, 917:135-145.
10. Siegelman H.W., Kycia J.H. Algal biliproteins. Hand Book of Phycological Methods-Physiological and Biochemical Methods 1978, 72-79. Cambridge University Press, Cambridge. J.A. Hellebust, J.S. Craige (Eds.).
11. Lawrenz E., Fedewa E.J., Richardson T.L. Extraction protocols for the quantification of phycobilins in aqueous phytoplankton extracts. J. Appl. Phycol. 2010, 23:865-871.
12. Hemlata, Pandey G., Bano F., Fatma T. Studies on Anabaena sp. NCCU-9 with special reference to phycocyanin. J. Algal Biomass Util. 2011, 2:30-51.
13. Niu J.F., Wang G.C., Tsen C.K. Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev. Protein Expr. Purif. 2006, 49:23-31.
14. O'Carra P. Purification and N-terminal analyses of algal biliproteins. Biochem. J. 1965, 94:171.
15. Dumay J., Clément N., Morançais M., Fleurence J. Optimization of hydrolysis conditions of Palmaria palmata to enhance R-phycoerythrin extraction. Bioresour. Technol. 2013, 131:21-27.
16. Häder D.P., Figueroa F.L. Photoecophysiology of marine macroalgae. Photochem. Photobiol. 1997, 66:1-14.
17. Senthilkumar N., Kurinjimalar C., Thangam R., Suresh V., Kavitha G., Gunasekaran P., Rengasamy R. Further studies and biological activities of macromolecular protein R-Phycoerythrin from Portieria hornemannii. Int. J. Biol. Macromol. 2013, 62:107-116.
18. Munier M., Jubeau S., Wijaya A., Morançais M., Dumay J., Marchal L., Jaouen P., Fleurence J. Physicochemical factors affecting the stability of two pigments: R-phycoerythrin of Grateloupia turuturu and B-phycoerythrin of Porphyridium cruentum. Food Chem. 2014, 150:400-407.
19. Dainippon InK and Chemicals Inc., Jpn. Pat. 95 (1979) 770.
20. Dainippon InK and Chemicals Inc., Jpn. Pat. 06 (1987) 691.
21. Santos M.C., Noyalo P.T., Olvera R.R. Extraction and purification of phycocyanin from Calothrix spp. Process Biochem. 2004, 39:2047-2052.
22. Mishra S.K., Shrivastav A., Mishra S. Effect of preservatives for food grade C-PC from Spirulina platensis. Process Biochem. 2008, 43:339-345.
23. Mishra S.K., Shrivastav A., Pancha I., Jain D., Mishra S. Effect of preservatives for food grade C-Phycoerythrin, isolated from marine cyanobacteria Pseudanabaena sp. Int. J. Biol. Macromol. 2010, 47:597-602.
24. Sekar S., Chandramohan M. Phycobiliproteins as a commodity: trends in applied research, patents and commercialization. J. Appl. Phycol. 2008, 20:113-136.
25. Denis C., Massé A., Fleurence J., Jaouen P. Concentration and pre-purification with ultrafiltration of a R-phycoerythrin solution extracted from macro-algae Grateloupia turuturu: process definition and up-scaling. Sep. Purif. Technol. 2009, 69:37-42.
26. Bennett, Bogorad L. Complementary chromatic adaptation in a filamentous blue-green alga. J. Cell Biol. 1973, 58:419-435.
27. Senthilkumar N., Suresh V., Thangam R., Kurinjimalar C., Kavitha G., Murugan P., Rengasamy R. Isolation and characterization of macromolecular protein R-phycoerythrin from Portieria hornemannii. Int. J. Biol. Macromol. 2013, 55:150-160.
28. Chen H., Dang W., Xie J., Zhao J., Weng Y. Ultrafast energy transfer pathways in R-phycoerythrin from Polysiphonia urceolata. Photosynth. Res. 2012, 111:81-86.
29. Baghel R.S., Kumari P., Reddy C.R.K., Jha B. Growth, pigments and biochemical composition of marine red alga Gracilaria crassa. J. Appl. Phycol. 2014, 10.1007/s10811-014-0250-5.
30. Glazer A.N., West J.A., Chan C. Phycoerythrins as chemotaxonomic markers in red algae: a survey. Biochem. Syst. Ecol. 1982, 10:203-215.
31. Chaiklahan R., Chirasuwan N., Bunnag B. Stability of phycocyanin extracted from Spirulina sp.: influence of temperature, pH and preservatives. Process Biochem. 2012, 47:659-664.
32. Sarada R., Pillai M.G., Ravishankar G.A. Phycocyanin from Spirulina sp.: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin. Process Biochem. 1999, 34:795-801.