Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda

BioMed Research International

Volumn 2015, Issue , 2015, Pages

  Mugizi, Denis Rwabiita ^a   Muradrasoli, Shaman ^b   Boqvist, Sofia ^b   Erume, Joseph ^a   Nasinyama, George William ^a   Waiswa, Charles ^a   Mboowa, Gerald ^a   Klint, Markus ^c   Magnusson, Ulf ^b  

^a MAKERERE UNIVERSITY   (Uganda)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

AFRICAN; ARTICLE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BIOTYPE; BOVINAE; BRUCELLA ABORTUS; BRUCELLOSIS; CONTROLLED STUDY; GENETIC VARIABILITY; GENOTYPE; GENOTYPING TECHNIQUE; MILK; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; UGANDA; VARIABLE NUMBER OF TANDEM REPEAT; ANIMAL; BACTERIUM IDENTIFICATION; BOVINE; DAIRY PRODUCT; GENETIC VARIATION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PROCEDURES;

BOS; BRUCELLA; BRUCELLA MELITENSIS BIOVAR ABORTUS;

ANIMALS; BACTERIAL TYPING TECHNIQUES; BRUCELLA ABORTUS; BRUCELLOSIS; CATTLE; DAIRY PRODUCTS; GENETIC VARIATION; GENOTYPE; MILK; MINISATELLITE REPEATS; UGANDA;

EID: 84924598089     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2015/720413     Document Type: Article

References (62)

1. J. Godfroid, H. C. Scholz, T. Barbier et al., "Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century," Preventive Veterinary Medicine, Vol. 102, no. 2, pp. 118-131, 2011.
2. J.-M. Verger, F. Grimont, P. A. D. Grimont, and M. Grayon, "Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization," International Journal of Systematic Bacteriology, Vol. 35, no. 3, pp. 292-295, 1985.
3. G. Pappas, "The changing Brucella ecology: novel reservoirs, new threats," International Journal of Antimicrobial Agents, Vol. 36, no. 1, pp. S8-S11, 2010.
4. D. Bruce, "Note on the discovery of a micro-organism in Malta fever," Practitioner, Vol. 39, pp. 161-170, 1887.
5. M. P. Franco, M. Mulder, R. H. Gilman, and H. L. Smits, "Human brucellosis," The Lancet Infectious Diseases, Vol. 7, no. 12, pp. 775-786, 2007.
6. H. C. Scholz, K. Nöckler, C. G. Llner et al., "Brucella inopinata sp. nov., isolated from a breast implant infection," International Journal of Systematic and Evolutionary Microbiology, Vol. 60, no. 4, pp. 801-808, 2010.
7. J. J. McDermott and S. M. Arimi, "Brucellosis in sub-Saharan Africa: epidemiology, control and impact," Veterinary Microbiology, Vol. 90, no. 1-4, pp. 111-134, 2002.
8. World Organisation for Animal Health and OIE, "Bovine brucellosis," in OIE Terrestrial Manual, chapter 2.4.3, 2009.
9. E. N. Muendo, P. M. Mbatha, J. Macharia et al., "Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes," Tropical Animal Health and Production, Vol. 44, no. 1, pp. 17-20, 2012.
10. D. R. Ewalt, J. B. Payeur, J. C. Rhyan, and P. L. Geer, "Brucella suis biovar 1 in naturally infected cattle: a bacteriological, sero-logical, and histological study," Journal of Veterinary Diagnostic Investigation, Vol. 9, no. 4, pp. 417-420, 1997.
11. G. G. Schurig, N. Sriranganathan, and M. J. Corbel, "Brucellosis vaccines: past, present and future," Veterinary Microbiology, Vol. 90, no. 1-4, pp. 479-496, 2002.
12. I. Moriyón, M. J. Grilló, D. Monreal et al., "Rough vaccines in animal brucellosis: structural and genetic basis and present status," Veterinary Research, Vol. 35, no. 1, pp. 1-38, 2004.
13. S. Valdezate, A. Navarro, P. Villalón, G. Carrasco, and J. A. Saéz-Nieto, "Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing," Journal of Clinical Microbiology, Vol. 48, no. 8, pp. 2734-2740, 2010.
14. M. E. Meyer and W. J. B. Morgan, "Designation of neotype strains and of biotype reference strains for species of the genus Brucella Meyer and Shaw," International Journal of Systematic Bacteriology, Vol. 23, no. 2, pp. 135-141, 1973.
15. A. M. S. Menshawy, M. Perez-Sancho, T. Garcia-Seco et al., "Assessment of genetic diversity of zoonotic Brucella spp. Recovered from livestock in Egypt using multiple locus VNTR analysis," BioMed Research International, Vol. 2014, Article ID 353876, 7 pages, 2014.
16. I. López-Goñi, D. García-Yoldi, C. M. Marín et al., "Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains," Journal of Clinical Microbiology, Vol. 46, no. 10, pp. 3484-3487, 2008.
17. P. le Flèche, I. Jacques, M. Grayon et al., "Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay," BMC Microbiology, Vol. 6, article 9, 2006.
18. G. Pappas, P. Papadimitriou, N. Akritidis, L. Christou, and E. V. Tsianos, "The new global map of human brucellosis," The Lancet Infectious Diseases, Vol. 6, no. 2, pp. 91-99, 2006.
19. R. D. Mugizi, S. Boqvist, and G. W. Nasinyama, "Prevalence of and factors associated with Brucella sero-positivity in cattle in urban and peri-urban farming systems in eastern and northern Uganda," Journal of Veterinary and Medical Science. In press.
20. K. Makita, E. M. Fèvre, C. Waiswa et al., "Human Brucellosis in urban and peri-urban areas of Kampala, Uganda," Annals of the New York Academy of Sciences, Vol. 1149, pp. 309-311, 2008.
21. K. Makita, E. M. Fèvre, C. Waiswa, M. C. Eisler, and S. C. Welburn, "How human brucellosis incidence in urban Kampala can be reduced most eficiently? A stochastic risk assessment of informally-marketed milk," PLoS ONE, Vol. 5, no. 12, Article ID e14188, 2010.
22. M. J. De Miguel, C. M. Marín, P. M. Muñoz, L. Dieste, M. J. Grilló, and J. M. Blasco, "Development of a selective culture medium for primary isolation of the main Brucella Species," Journal of Clinical Microbiology, Vol. 49, no. 4, pp. 1458-1463, 2011.
23. W. S. Probert, K. N. Schrader, N. Y. Khuong, S. L. Bystrom, and M. H. Graves, "Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis," Journal of Clinical Microbiology, Vol. 42, no. 3, pp. 1290-1293, 2004.
24. G. Borriello, S. Peletto, M. G. Lucibelli, P. L. Acutis, D. Ercolini, and G. Galiero, "Link between geographical origin and occurrence of Brucella abortus biovars in cow and water buffalo herds," Applied and Environmental Microbiology, Vol. 79, no. 3, pp. 1039-1043, 2013.
25. S. Al Dahouk, P. L. Flèche, K. Nöckler et al., "Evaluation of Brucella MLVA typing for human brucellosis," Journal of Microbiological Methods, Vol. 69, no. 1, pp. 137-145, 2007.
26. A. M. Whatmore, S. J. Shankster, L. L. Perrett et al., "Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp," Journal of Clinical Microbiology, Vol. 44, no. 6, pp. 1982-1993, 2006.
27. M. Tittarelli, M. di Ventura, F. de Massis et al., "The persistence of Brucella melitensis in experimentally infected ewes through three reproductive cycles," Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, Vol. 52, no. 9, pp. 403-409, 2005.
28. R. Capparelli, M. Parlato, M. Iannaccone et al., "Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis," Journal of Applied Microbiology, Vol. 106, no. 6, pp. 2041-2047, 2009.
29. M. T. Musa, K. L. Jahans, and M. E. Fadalla, "Brucella biovars isolated from nomadic cattle in the southern Darfur Province of Western Sudan," Journal of Comparative Pathology, Vol. 102, no. 1, pp. 49-54, 1990.
30. R. Redkar, S. Rose, B. Bricker, and V. Delvecchio, "Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis," Molecular and Cellular Probes, Vol. 15, no. 1, pp. 43-52, 2001.
31. B. Garin-Bastuji, V. Mick, G. Le Carrou et al., "Examination of taxonomic uncertainties surrounding Brucella abortus bv. 7 by phenotypic and molecular approaches," Applied and Environmental Microbiology, Vol. 80, no. 5, pp. 1570-1579, 2014.
32. J. Godfroid, H. C. Scholz, T. Barbier et al., "Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century," Preventive Veterinary Medicine, Vol. 102, no. 2, pp. 118-131, 2011.
33. J.-M. Verger, F. Grimont, P. A. D. Grimont, and M. Grayon, "Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization," International Journal of Systematic Bacteriology, Vol. 35, no. 3, pp. 292-295, 1985.
34. G. Pappas, "The changing Brucella ecology: novel reservoirs, new threats," International Journal of Antimicrobial Agents, Vol. 36, no. 1, pp. S8-S11, 2010.
35. D. Bruce, "Note on the discovery of a micro-organism in Malta fever," Practitioner, Vol. 39, pp. 161-170, 1887.
36. M. P. Franco, M. Mulder, R. H. Gilman, and H. L. Smits, "Human brucellosis," The Lancet Infectious Diseases, Vol. 7, no. 12, pp. 775-786, 2007.
37. H. C. Scholz, K. Nöckler, C. G. Llner et al., "Brucella inopinata sp. nov., isolated from a breast implant infection," International Journal of Systematic and Evolutionary Microbiology, Vol. 60, no. 4, pp. 801-808, 2010.
38. J. J. McDermott and S. M. Arimi, "Brucellosis in sub-Saharan Africa: epidemiology, control and impact," Veterinary Microbiology, Vol. 90, no. 1-4, pp. 111-134, 2002.
39. World Organisation for Animal Health and OIE, "Bovine brucellosis," in OIE Terrestrial Manual, chapter 2.4.3, 2009.
40. E. N. Muendo, P. M. Mbatha, J. Macharia et al., "Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes," Tropical Animal Health and Production, Vol. 44, no. 1, pp. 17-20, 2012.
41. D. R. Ewalt, J. B. Payeur, J. C. Rhyan, and P. L. Geer, "Brucella suis biovar 1 in naturally infected cattle: a bacteriological, sero-logical, and histological study," Journal of Veterinary Diagnostic Investigation, Vol. 9, no. 4, pp. 417-420, 1997.
42. G. G. Schurig, N. Sriranganathan, and M. J. Corbel, "Brucellosis vaccines: past, present and future," Veterinary Microbiology, Vol. 90, no. 1-4, pp. 479-496, 2002.
43. I. Moriyón, M. J. Grilló, D. Monreal et al., "Rough vaccines in animal brucellosis: structural and genetic basis and present status," Veterinary Research, Vol. 35, no. 1, pp. 1-38, 2004.
44. S. Valdezate, A. Navarro, P. Villalón, G. Carrasco, and J. A. Saéz-Nieto, "Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing," Journal of Clinical Microbiology, Vol. 48, no. 8, pp. 2734-2740, 2010.
45. M. E. Meyer and W. J. B. Morgan, "Designation of neotype strains and of biotype reference strains for species of the genus Brucella Meyer and Shaw," International Journal of Systematic Bacteriology, Vol. 23, no. 2, pp. 135-141, 1973.
46. A. M. S. Menshawy, M. Perez-Sancho, T. Garcia-Seco et al., "Assessment of genetic diversity of zoonotic Brucella spp. Recovered from livestock in Egypt using multiple locus VNTR analysis," BioMed Research International, Vol. 2014, Article ID 353876, 7 pages, 2014.
47. I. López-Goñi, D. García-Yoldi, C. M. Marín et al., "Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains," Journal of Clinical Microbiology, Vol. 46, no. 10, pp. 3484-3487, 2008.
48. P. le Flèche, I. Jacques, M. Grayon et al., "Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay," BMC Microbiology, Vol. 6, article 9, 2006.
49. G. Pappas, P. Papadimitriou, N. Akritidis, L. Christou, and E. V. Tsianos, "The new global map of human brucellosis," The Lancet Infectious Diseases, Vol. 6, no. 2, pp. 91-99, 2006.
50. R. D. Mugizi, S. Boqvist, and G. W. Nasinyama, "Prevalence of and factors associated with Brucella sero-positivity in cattle in urban and peri-urban farming systems in eastern and northern Uganda," Journal of Veterinary and Medical Science. In press.
51. K. Makita, E. M. Fèvre, C. Waiswa et al., "Human Brucellosis in urban and peri-urban areas of Kampala, Uganda," Annals of the New York Academy of Sciences, Vol. 1149, pp. 309-311, 2008.
52. K. Makita, E. M. Fèvre, C. Waiswa, M. C. Eisler, and S. C. Welburn, "How human brucellosis incidence in urban Kampala can be reduced most eficiently? A stochastic risk assessment of informally-marketed milk," PLoS ONE, Vol. 5, no. 12, Article ID e14188, 2010.
53. M. J. De Miguel, C. M. Marín, P. M. Muñoz, L. Dieste, M. J. Grilló, and J. M. Blasco, "Development of a selective culture medium for primary isolation of the main Brucella Species," Journal of Clinical Microbiology, Vol. 49, no. 4, pp. 1458-1463, 2011.
54. W. S. Probert, K. N. Schrader, N. Y. Khuong, S. L. Bystrom, and M. H. Graves, "Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis," Journal of Clinical Microbiology, Vol. 42, no. 3, pp. 1290-1293, 2004.
55. G. Borriello, S. Peletto, M. G. Lucibelli, P. L. Acutis, D. Ercolini, and G. Galiero, "Link between geographical origin and occurrence of Brucella abortus biovars in cow and water buffalo herds," Applied and Environmental Microbiology, Vol. 79, no. 3, pp. 1039-1043, 2013.
56. S. Al Dahouk, P. L. Flèche, K. Nöckler et al., "Evaluation of Brucella MLVA typing for human brucellosis," Journal of Microbiological Methods, Vol. 69, no. 1, pp. 137-145, 2007.
57. A. M. Whatmore, S. J. Shankster, L. L. Perrett et al., "Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp," Journal of Clinical Microbiology, Vol. 44, no. 6, pp. 1982-1993, 2006.
58. M. Tittarelli, M. di Ventura, F. de Massis et al., "The persistence of Brucella melitensis in experimentally infected ewes through three reproductive cycles," Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, Vol. 52, no. 9, pp. 403-409, 2005.
59. R. Capparelli, M. Parlato, M. Iannaccone et al., "Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis," Journal of Applied Microbiology, Vol. 106, no. 6, pp. 2041-2047, 2009.
60. M. T. Musa, K. L. Jahans, and M. E. Fadalla, "Brucella biovars isolated from nomadic cattle in the southern Darfur Province of Western Sudan," Journal of Comparative Pathology, Vol. 102, no. 1, pp. 49-54, 1990.
61. R. Redkar, S. Rose, B. Bricker, and V. Delvecchio, "Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis," Molecular and Cellular Probes, Vol. 15, no. 1, pp. 43-52, 2001.
62. B. Garin-Bastuji, V. Mick, G. Le Carrou et al., "Examination of taxonomic uncertainties surrounding Brucella abortus bv. 7 by phenotypic and molecular approaches," Applied and Environmental Microbiology, Vol. 80, no. 5, pp. 1570-1579, 2014.