Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains

Journal of Clinical Microbiology

Volumn 46, Issue 10, 2008, Pages 3484-3487

  Lopez Goni I ^a   Garcia Yoldi D ^a   Marin C M ^b   De Miguel, M J ^b   Munoz P M ^b   Blasco, J M ^b   Jacques, I ^c,d   Grayon, M ^d   Cloeckaert, A ^d   Ferreira, A C ^e   Cardoso, R ^e   Correa De Sa M I ^e   Walravens, K ^f   Albert, D ^g   Garin Bastuji, B ^g  

^a UNIVERSITY OF NAVARRA   (Spain)

^b Agrifood Institute of Aragón IA2   (Spain)

^c UNIVERSITÉ DE TOURS   (France)

^d UR1282   (France)

^e Laboratorio Nacional de Investigacao Veterinaria   (Portugal)

^f VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

^g Healthy Pigs Production and Experimentation Section   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL VACCINE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; BRUCELLA; BRUCELLOSIS; DNA EXTRACTION; DNA SEQUENCE; GENETIC DIFFERENCE; HUMAN; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ANIMALS; BACTERIAL TYPING TECHNIQUES; BRUCELLA; DNA PRIMERS; HUMANS; MAMMALS; POLYMERASE CHAIN REACTION;

ANIMALIA; BRUCELLA; MAMMALIA;

EID: 53649106971     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00837-08     Document Type: Article

References (17)

1. Alton, G. G., L. M. Jones, R. D. Angus, and J. M. Verger. 1988. Techniques for the brucellosis laboratory. INRA, Paris, France.
2. Bricker, B. J., D. R. Ewalt, S. C. Olsen, and A. E. Jensen. 2003. Evaluation of the Brucella abortus species-specific polymerase chain reaction assay, an improved version of the Brucella AMOS polymerase chain reaction assay for cattle. J. Vet. Diagn. Investig. 15:374-378.
3. Bricker, B. J., and S. M. Halling. 1994. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 32:2660-2666.
4. Bricker, B. J., and S. M. Halling. 1995. Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51. J. Clin. Microbiol. 33:1640-1642.
5. Cloeckaert, A., M. Grayon, and O. Grepinet. 2000. An IS711 element downstream of the bp26 gene is a specific marker of Brucella spp. isolated from marine mammals. Clin. Diagn. Lab. Immunol. 7:835-839.
6. Cloeckaert, A., M. Grayon, and O. Grepinet. 2002. Identification of Brucella melitensis vaccine strain Rev.1 by PCR-RFLP based on a mutation in the rpsL gene. Vaccine 20:2546-2550.
7. Corbel, M. J. 1988. International committee on the systematic bacteriology-subcommittee on the taxonomy of Brucella. Int. J. Syst. Bacteriol. 38:450-452.
8. Foster, G., B. S. Osterman, J. Godfroid, I. Jacques, and A. Cloeckaert. 2007. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol. 57:2688-2693.
9. García-Yoldi, D., C. M. Marín, and I. López-Goñi. 2005. Restriction site polymorphisms in the genes encoding new members of group 3 outer membrane protein family of Brucella spp. FEMS Microbiol. Lett. 245:79-84.
10. García-Yoldi, D., C. M. Marín, M. J. De Miguel, P. M. Muñoz, J. L. Vizmanos, and I. López-Goñi. 2006. Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin. Chem. 52:779-781.
11. Halling, S. M., B. D. Peterson-Burch, B. J. Bricker, R. L. Zuerner, Z. Qing, L. L. Li, V. Kapur, D. P. Alt, and S. S. Olsen. 2005. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J. Bacteriol. 187:2715-2726.
12. Paulsen, I. T., R. Seshadri, K. E. Nelson, J. A. Eisen, J. F. Heidelberg, T. D. Read, R. J. Dodson, L. Umayam, L. M. Brinkac, M. J. Beanan, S. C. Daugherty, R. T. Deboy, A. S. Durkin, J. F. Kolonay, R. Madupu, W. C. Nelson, B. Ayodeji, M. Kraul, J. Shetty, J. Malek, S. E. Van Aken, S. Riedmuller, H. Tettelin, S. R. Gill, O. White, S. L. Salzberg, D. L. Hoover, L. E. Lindler, S. M. Halling, S. M. Boyle, and C. M. Fraser. 2002. The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. Proc. Natl. Acad. Sci. USA 99:13148-13153.
13. Rajashekara, G., J. D. Glasner, D. A. Glover, and G. A. Splitter. 2004. Comparative whole-genome hybridization reveals genomic islands in Brucella species. J. Bacteriol. 186:5040-5051.
14. Sangari, F. J., J. M. García-Lobo, and J. Agüero. 1994. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol. Lett. 121:337-342.
15. Vemulapalli, R., J. R. McQuiston, G. G. Schurig, N. Sriranganathan, S. M. Halling, and S. M. Boyle. 1999. Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains. Clin. Diagn. Lab. Immunol. 6:760-764.
16. Verger, J. M., F. Grimont, P. A. D. Grimont, and M. Grayon. 1985. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol. 35:292-295.
17. Vizcaíno, N., J. M. Verger, M. Grayon, M. S. Zygmunt, and A. Cloeckaert. 1997. DNA polymorphism at the omp-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus, and other species-specific markers. Microbiology 143:2913-2921.