A comparison of glucose concentration in paired specimens collected in serum separator and fluoride/potassium oxalate blood collection tubes under survey 'field' conditions

Clinical Biochemistry

Volumn 46, Issue 4-5, 2013, Pages 285-288

  Fernandez, Lois ^a   Jee, Penny ^a   Klein, Mari Jill ^a   Fischer, Peter ^a   Perkins, Sherry L ^b,c   Brooks, Stephen P J ^a  

^a Bureau of Nutritional Sciences   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

^c UNIVERSITY OF OTTAWA   (Canada)

Author keywords

Blood glucose; Fluoride oxalate; Serum separator

Indexed keywords

CATALASE; FLUORIDE; GLUCOSE; GLUCOSE OXIDASE; OXALATE POTASSIUM; POTASSIUM; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BLOOD SAMPLING; ENZYME RELEASE; ERYTHROCYTE; FEMALE; GLUCOSE BLOOD LEVEL; HEALTH SURVEY; HEMOLYSIS; HUMAN; MAJOR CLINICAL STUDY; MALE; MEDICAL DEVICE; POTASSIUM BLOOD LEVEL; PRIORITY JOURNAL; SERUM SEPARATOR TUBE;

ANTICOAGULANTS; BLOOD GLUCOSE; BLOOD SPECIMEN COLLECTION; HEMOLYSIS; HUMANS; OXALIC ACID; REDUCING AGENTS; SODIUM FLUORIDE;

EID: 84873731597     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2012.11.027     Document Type: Article

References (14)

1. Astles R., Williams C.P., Sedor F. Stability of plasma lactate in vitro in the presence of antiglycolytic agents. Clin Chem 1994, 40:1327-1330.
2. Rosa-Fraile M., Sampedro A., Rodriguez-Granger J., Camacho E., Manrique E. Suitability of frozen serum stored in gel separator primary sampling tubes for serological testing. Clin Diagn Lab Immunol 2004, 11:219-221.
3. Gambino R., Piscitelli J., Ackattupathil T.A., Theriault J.L., Andrin R.D., Sanfilippo M.L., et al. Acidification of blood is superior to sodium fluoride alone as an inhibitor of glycolysis. Clin Chem 2009, 55:1019-1021.
4. Christopher M.M., O'Neill S. Effect of specimen collection and storage on blood glucose and lactate concentrations in healthy, hyperthyroid and diabetic cats. Vet Clin Pathol 2000, 29:22-28.
5. Day B., Langlois R., Tremblay M., Knoppers B.-M. Canadian health measures survey: ethical, legal and social issues. Health Rep 2007, 18:S37-S51.
6. Young D.S., Bermes E.W.J., Haverstick D.M. Specimen collection and other preanalytical variables. Tietz fundamentals of clinical chemistry 2008, 42-49. Saunders Elsevier, St. Louis, MO. 6th ed. C.A. Burtis, E.R. Ashwood, D.E. Bruns (Eds.).
7. Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1969, 6:24-27.
8. Curme H.G., Columbus R.L., Dappen G.M., Eder T.W., Fellows W.D., Figueras J., et al. Multilayer film elements for clinical analysis: general concepts. Clin Chem 1978, 24:1335-1342.
9. Blasutig IM, Jung B, Kulasingam V, Baradaran S, Chen Y, Chan MK, et al. Evaluation of the vitros 5600 integrated system in a pediatric setting and determination of pediatric reference intervals. Clin Biochem 2010;43:1039-44.
10. Holtkamp H.C., Verhoef N.J., Leijnse B. The difference between the glucose concentrations in plasma and whole blood. Clin Chim Acta 1975, 59:41-49.
11. Vesper H.W., Archibold E., Porter K.H., Myers G.L. Assessment of a reference procedure to collect and analyze glucose in capillary whole blood. Clin Chem 2005, 51:901-903.
12. Sacks D.B., Bruns D.E., Goldstein D.E., Maclaren N.K., McDonald J.M., Parrott M. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Clin Chem 2002, 48:436-472.
13. Waring W.S., Evans L.E., Kirkpatrick C.T. Glycolysis inhibitors negatively bias blood glucose measurements: potential impact on the reported prevalence of diabetes mellitus. J Clin Pathol 2007, 60:820-823.
14. Shi R.Z., Seeley E.S., Bowen R., Faix J.D. Rapid blood separation is superior to fluoride for preventing in vitro reductions in measured blood glucose concentration. J Clin Pathol 2009, 62:752-753.