Molecular cloning, characterization and expression of lipoxygenase 2 (lox-2) isozyme from Indian soybean [Glycine max (L.) Merrill] cv. Pusa 16

Indian Journal of Biochemistry and Biophysics

Volumn 50, Issue 1, 2013, Pages 54-63

  Mandal, Somnath ^a,b   Sahana, Nandita ^a   Rajarani, A P ^a   Santha, I M ^a  

^a INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

^b JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

cDNA cloning; Glycine max (L.) Merril; Hydropathy profile; Inhibitors; Lipoxygenase; Prokaryotic expression; Recombinant enzyme kinetics; Soybean

Indexed keywords

LIPOXYGENASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; INDIA; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SOYBEAN;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; INDIA; LIPOXYGENASE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SOYBEANS;

DICOTYLEDONEAE; GLYCINE MAX; PROKARYOTA; PUSA;

EID: 84924246367     PISSN: 03011208     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Siedow J N (1991) Annu Rev Plant Physiol Plant Mol Biol 42, 145-188.
2. Feussner I, Kuhn H & Wasternack C (2001) Trends Plant Sci 6, 268-273.
3. Mack A J, Peterman T K & Siedow J N (1987) Curr Top Biol Med Res 13, 127-154.
4. Loiseau J, Benoîtl V, Macherel M H & Le Deunff Y (2001) Seed Sci Res 11, 199-211.
5. Maccarrone M, Vanaarie P G M, Veldink G & Vliegenthart J F G (1994) Biochim Biophys Acta 1190, 164-169.
6. Ramadoss C S, Pistorius E K & Axelrod B (1978) Arch Biochem Biophys 190, 549-552.
7. Shibata D, Slusarenko A, Casey R, Hilderand D & Bell E (1994) Plant Mol Biol Rep 12, 41-42.
8. Feussner I & Wasternack C (2002) Annu Rev Plant Biol 53, 275-297.
9. Mandal S (2012) Functional characterization of lipoxygenase isozymes in relation to flavour development in soybean (Glycine max), Ph.D thesis submitted to P. G. School, IARI, New Delhi
10. Gowda S K & Minton N P (1995) Nucleic acid Res 23, 3357-3358.
11. Hall T A (1999) Nucleic Acids Symp Ser 41, 95-98.
12. Thompson J D, Gibson T J, Plewniak F, Jeanmougin F & Higgins D G (1997) Nucleic Acid Res 24, 4876-4882.
13. Felsenstein J (1985) Evolution 39, 783-791.
14. Rodriguez P L & Carrasco L (1993) J Biol Chem 268, 8105-8110.
15. Laemmli U K (1970) Nature 227, 680-685.
16. Axelrod B, Cheesebourgh T M & Laakso C (1981) Methods Enzymol 71, 441-451.
17. Shibata D, Stecsko J, Dixon J E, Andrews P C, Hermodson M & Axelrod B (1988) J Biol Chem 263, 6816-6821.
18. Kyte J & Doolittle R F (1982) J Mol Biol 157,105-132.
19. Minor W, Steczko J, Stec B, Otwinowski Z, Bolin J T, Walter R & Axelrod B (1996) Biochemistry 35, 10687-10701.
20. Wang W H, Takano T, Shibata D, Kitamura K & Takeda G (1994) Proc Natl Acad Sci (USA) 91, 5828-5832.
21. Steczko J, Donoho G A, Dixon J E, Sugimoto T & Axelrod B (1991) Protein Expr Purif 2, 221-227.
22. Matoba T, Hidaka H, Kitamura K, Kaizuma N & Kito M (1985) J Agric Food Chem 33, 856-858.
23. Grossman S & Waksman E G (1984) Int J Biochim 16, 281-289.
24. Davies C S, Nielsen S S & Nielsen N C (1987) J Am Oil Chem Soc 64, 1428-1433.
25. Blee E (2002) Trends Plant Sci 7, 315-322