Increased sample volume and use of quantitative reverse-transcription PCR can improve prediction of liver-to-blood inoculum size in controlled human malaria infection studies

Malaria Journal

Volumn 14, Issue 1, 2015, Pages

  Hodgson, Susanne H ^a   Douglas, Alexander D ^a   Edwards, Nick J ^a   Kimani, Domtila ^c   Elias, Sean C ^a   Chang, Ming ^b   Daza, Glenda ^b   Seilie, Annette M ^b   Magiri, Charles ^d   Muia, Alfred ^d   Juma, Elizabeth A ^d,e   Cole, Andrew O ^d   Rampling, Thomas W ^a   Anagnostou, Nicholas A ^a   Gilbert, Sarah C ^a   Hoffman, Stephen L ^f   Draper, Simon J ^a   Bejon, Philip ^c   Ogutu, Bernhards ^d,e   Marsh, Kevin ^c   Hill, Adrian V S ^a   Murphy, Sean C ^b   more..

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF WASHINGTON   (United States)

^c KEMRI WELLCOME TRUST RESEARCH PROGRAMME   (Kenya)

^d KENYA MEDICAL RESEARCH INSTITUTE   (Kenya)

^e STRATHMORE UNIVERSITY   (Kenya)

^f SANARIA INC   (United States)

Author keywords

18S rRNA; Calibrators; Controls; PCR; Plasmodium falciparum; Pre erythrocytic; RT PCR; Standards

Indexed keywords

MALARIA VACCINE; RIBOSOME DNA; RNA 18S; PROTOZOAL DNA;

ADULT; ARTICLE; BLOOD SAMPLING; BLOOD VOLUME; CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC TEST; DIAGNOSTIC TEST ACCURACY STUDY; DRIED BLOOD SPOT TESTING; DRUG EFFICACY; FEMALE; HUMAN; IMMUNIZATION; INOCULATION; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LIVER; LIVER TO BLOOD INOCULUM; MAJOR CLINICAL STUDY; MALARIA FALCIPARUM; MALE; NUCLEIC ACID ANALYSIS; NUCLEIC ACID TESTING; PARASITE CONTROL; PARASITEMIA; PLASMODIUM FALCIPARUM; PREDICTIVE VALUE; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UNITED STATES; BLOOD; GENETICS; MALARIA; MIDDLE AGED; MOLECULAR DIAGNOSIS; PARASITOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES; YOUNG ADULT;

ADULT; DNA, PROTOZOAN; FEMALE; HUMANS; LIMIT OF DETECTION; MALARIA; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; PARASITEMIA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; YOUNG ADULT;

EID: 84924124966     PISSN: None     EISSN: 14752875     Source Type: Journal    
DOI: 10.1186/s12936-015-0541-6     Document Type: Article

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