Direct detection of markers associated with Neisseria gonorrhoeae antimicrobial resistance in New Zealand using residual DNA from the Cobas 4800 CT/NG NAAT assay

Sexually Transmitted Infections

Volumn 91, Issue 2, 2015, Pages 91-93

  Nicol, Mackenzie ^a   Whiley, David ^b   Nulsen, Mary ^c   Bromhead, Collette ^a  

^a Aotea Pathology Ltd   (New Zealand)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c MASSEY UNIVERSITY   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

CEFTRIAXONE; CIPROFLOXACIN; DNA TOPOISOMERASE (ATP HYDROLYSING) A; PENICILLIN DERIVATIVE; QUINOLONE; GENETIC MARKER;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; CODON; GENETIC MARKER; GENOTYPE; MINIMUM INHIBITORY CONCENTRATION; NEISSERIA GONORRHOEAE; NEW ZEALAND; NONHUMAN; NUCLEIC ACID AMPLIFICATION; PENA GENE; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM; DRUG EFFECTS; EVALUATION STUDY; FEMALE; GENETICS; HUMAN; MALE; MICROBIAL SENSITIVITY TEST; MOLECULAR DIAGNOSIS; PROCEDURES;

DRUG RESISTANCE, BACTERIAL; FEMALE; GENES, BACTERIAL; GENETIC MARKERS; GENOTYPE; HUMANS; MALE; MICROBIAL SENSITIVITY TESTS; MOLECULAR DIAGNOSTIC TECHNIQUES; NEISSERIA GONORRHOEAE; NEW ZEALAND; NUCLEIC ACID AMPLIFICATION TECHNIQUES;

EID: 84923973208     PISSN: 13684973     EISSN: 14723263     Source Type: Journal    
DOI: 10.1136/sextrans-2014-051632     Document Type: Article

References (10)

1. st century: past, evolution, and future. Clin Microbiol Rev 2014;27:587-613.
2. Ochiai S, Ishiko H, Yasuda M, et al. Rapid detection of the mosaic structure of the Neisseria gonorrhoeae penA gene, which is associated with decreased susceptibilities to oral cephalosporins. J Clin Microbiol 2008;46:1804-10.
3. Whiley DM, Goire N, Lambert SB, et al. Reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae is spread internationally by genetically distinct gonococcal populations. J Antimicrob Chemother 2011;66:1186-7.
4. Bromhead C, Miller A, Jones M. Neisseria gonorrhoeae testing in New Zealand, culture or PCR? NZ J Med Lab Sci 2013;67:4-6.
5. Whiley DM, Goire N, Lahra MM, et al. The ticking time bomb: escalating antibiotic resistance in Neisseria gonorrhoeae is a public health disaster in waiting. J Antimicrob Chemother 2012;67:2059-61.
6. Siedner MJ, Pandori M, Castro L, et al. Real-time PCR assay for detection of quinolone-resistant Neisseria gonorrhoeae in urine samples. J Clin Microbiol 2007;45:1250-4.
7. Goire N, Freeman K, Tapsall JW, et al. Enhancing gonococcal antimicrobial resistance surveillance: a real-time PCR assay for detection of penicillinase-producing Neisseria gonorrhoeae by use of noncultured clinical samples. J Clin Microbiol 2011;49:513-8.
8. Bromhead C, Miller A, Jones M, et al. Comparison of the cobas 4800 CT/NG test with culture for detecting Neisseria gonorrhoeae in genital and nongenital specimens in a low-prevalence population in New Zealand. J Clin Microbiol 2013;51:1505-9.
9. Goire N, Nissen MD, LeCornec GM, et al. A duplex Neisseria gonorrhoeae real-time polymerase chain reaction assay targeting the gonococcal porA pseudogene and multicopy opa genes. Diagn Microbiol Infect Dis 2008;61:6-12.
10. Sexually transmitted infections in New Zealand: annual surveillance report 2012. Porirua, New Zealand: The Institute of Environmental Science and Research Ltd. https://surv.esr.cri.nz/PDF-surveillance/STISurvRpt/2012/2012AnnualSTIReport.pdf