Hepatitis B virus infection and replication in a new cell culture system established by fusing HepG2 cells with primary human hepatocytes

Journal of Microbiology, Immunology and Infection

Volumn 49, Issue 4, 2016, Pages 471-476

  Sai, Lin Tao ^a   Yao, Yong Yuan ^b   Guan, Yan Yan ^b   Shao, Li Hua ^c   Ma, Rui Ping ^d   Ma, Li Xian ^a  

^a SHANDONG UNIVERSITY   (China)

^b PEOPLE S HOSPITAL OF RIZHAO   (China)

^c SHANDONG UNIVERSITY   (China)

^d THE FIRST AFFILIATED HOSPITAL OF SHANDONG FIRST MEDICAL UNIVERSITY   (China)

Author keywords

Hepatitis B virus; Hybrid cells; Infection; Replication

Indexed keywords

CIRCULAR DNA; HEPATITIS B CORE ANTIGEN; HEPATITIS B SURFACE ANTIGEN; HEPATITIS B(E) ANTIGEN; VIRUS DNA;

ARTICLE; CELL FUSION; CHEMOLUMINESCENCE; CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; FLUORESCENCE ANALYSIS; HEPATITIS B; HEPCHLINE 7 CELL LINE; HEPG2 CELL LINE; HUMAN; HUMAN CELL; HUMAN TISSUE; HYBRIDOMA CELL LINE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; LIVER CELL; NONHUMAN; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; VIRUS REPLICATION; CELL CULTURE TECHNIQUE; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; HEP-G2 CELL LINE; HEPATITIS B VIRUS; HYBRID CELL; PROCEDURES; TUMOR CELL LINE; VIROLOGY;

CELL CULTURE TECHNIQUES; CELL FUSION; CELL LINE, TUMOR; DNA, CIRCULAR; DNA, VIRAL; HEP G2 CELLS; HEPATITIS B CORE ANTIGENS; HEPATITIS B E ANTIGENS; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VIRUS; HEPATOCYTES; HUMANS; HYBRID CELLS;

EID: 84923878880     PISSN: 16841182     EISSN: 19959133     Source Type: Journal    
DOI: 10.1016/j.jmii.2014.08.008     Document Type: Article

References (21)

1. 1 Shepard, C.W., Simard, E.P., Finelli, L., Fiore, A.E., Bell, B.P., Hepatitis B virus infection: epidemiology and vaccination. Epidemiol Rev 28 (2006), 112–125.
2. 2 World Health Organization, Hepatitis B. (Fact sheet no. 204). 2000, World Health Organization, Geneva.
3. 3 Beasley, R.P., Hwang, L.Y., Lin, C.C., Chien, C.S., Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan. Lancet 2 (1981), 1129–1133.
4. 4 Lok, A.S., Chronic hepatitis B. N Engl J Med 346 (2002), 1682–1683.
5. 5 Lu, X., Block, T., Gerlich, W.H., Protease-induced infectivity of hepatitis B virus for a human hepatoma cell line. J Virol 70 (1996), 2277–2285.
6. 6 Lu, X., Hazboun, T., Block, T., Limited proteolysis induces woodchuck hepatitis virus infectivity for human HepG2 cells. Virus Research 73 (2001), 27–40.
7. 7 Tuttleman, J.S., Pourcel, C., Summers, J., Formation of the pool of covalently closed circular viral DNA in hepadnavirus-infected cells. Cell 47 (1986), 451–460.
8. 8 Fellig, Y., Almogy, G., Galun, E., Ketzinel-Gilad, M., A hepatocellular carcinoma cell line producing mature hepatitis B viral particles. Biochem Biophys Res Commun 321 (2004), 269–274.
9. 9 Chin, R., Nachbur, U., Earnest-Silveira, L., Bankovacki, A., Koeberlein, B., Zentgraf, H., et al. Dysregulation of hepatocyte cell cycle and cell viability by hepatitis B virus. Virus Res 147 (2010), 7–16.
10. 10 Gripon, P., Rumin, S., Urban, S., Le Seyec, J., Glaise, D., Cannie, I., et al. Infection of a human hepatoma cell line by hepatitis B virus. Proc Natl Acad Sci USA 99 (2002), 15655–15660.
11. 11 Paran, N., Geiger, B., Shaul, Y., HBV infection of cell culture: evidence for multivalent and cooperative attachment. EMBO J 20 (2001), 4443–4453.
12. 12 Gripon, P., Diot, C., Theze, N., Fourel, I., Loreal, O., Brechot, C., et al. Hepatitis B virus infection of adult human hepatocytes cultured in the presence of dimethyl sulfoxide. J Virol 62 (1988), 4136–4143.
13. 13 Gripon, P., Diot, C., Guguen-Guillouzo, C., Reproducible high level infection of cultured adult human hepatocytes by hepatitis B virus: effect of polyethylene glycol on adsorption and penetration. Virology 192 (1993), 534–540.
14. 14 Baccarani, U., Sanna, A., Cariani, A., Sainz-Barriga, M., Adani, G.L., Zambito, A.M., et al. Isolation of human hepatocytes from livers rejected for liver transplantation on a national basis: results of a 2-year experience. Liver Transpl 9 (2003), 506–512.
15. 15 Guguen-Guillouzo, C., Campion, J.P., Brissot, P., Glaise, D., Launois, B., Bourel, M., et al. High yield preparation of isolated human adult hepatocytes by enzymatic perfusion of the liver. Cell Biol Int Rep 6 (1982), 625–628.
16. 16 Littlefield, J.W., Three degrees of guanyl acid inosinic acid pyrophosphorylase deficiency in mouse fibroblasts. Nature 203 (1964), 1142–1144.
17. 17 Smyth, D.R., Evans, H.J., Mapping of sister-chromatid exchanges in human chromosomes using G-banding and autoradiography. Mutat Res 35 (1976), 139–154.
18. 18 Klintschar, M., Neuhuber, F., Evaluation of an alkaline lysis method for the extraction of DNA from whole blood and forensic stains for STR analysis. J Forensic Sci 45 (2000), 669–673.
19. 19 Summers, J., Smith, P.M., Horwich, A.L., Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification. J Virol 64 (1990), 2819–2824.
20. 20 Yang, W., Mason, W.S., Summers, J., Covalently closed circular viral DNA formed from two types of linear DNA in woodchuck hepatitis virus-infected liver. J Virol 70 (1996), 4567–4575.
21. 21 He, M.L., Wu, J., Chen, Y., Lin, M.C., Lau, G.K., Kung, H.F., A new and sensitive method for the quantification of HBV cccDNA by realtime PCR. Biochem Biophys Res Commun 295 (2002), 1102–1107.