Identification and staining of distinct populations of secretory organelles in astrocytes

Cold Spring Harbor Protocols

Volumn 2014, Issue 5, 2014, Pages 532-536

  Bezzi, Paola ^a   Volterra, Andrea ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; VESICULAR GLUTAMATE TRANSPORTER 1; VESICULAR GLUTAMATE TRANSPORTER 2;

ARTICLE; ASTROCYTE; DENSE CORE GRANULE; ENDOCYTOSIS; EXOCYTOSIS; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; LYSOSOME; PROTEIN EXPRESSION; SECRETORY VESICLE; STAINING; SYNAPTIC LIKE MICROVESICLE;

EID: 84923864339     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot081703     Document Type: Article

References (22)

1. Bergersen LH, Gundersen V. 2009. Morphological evidence for vesicular glutamate release from astrocytes. Neuroscience 158: 2–60.
2. Bezzi P, Volterra A. 2014. Imaging exocytosis and recycling of synaptic-like microvesicles in astrocytes. Cold Spring Harb Protoc doi: 10.1101/pdb. prot081711.
3. Bezzi P, Gundersen V, Galbete JL, Seifert G, Steinhäuser C, Pilati E, Volterra A. 2004. Astrocytes contain a vesicular compartment that is competent for regulated exocytosis of glutamate. Nat Neurosci 7: 6–13.
4. Calegari F, Coco S, Taverna E, Bassetti M, Verderio C, Corradi N, Matteoli M, Rosa P. 1999. A regulated secretory pathway in cultured hippocampal astrocytes. J Biol Chem 274: 2–2539.
5. Coco S, Calegari F, Pravettoni E, Pozzi D, Taverna E, Rosa P, Matteoli M, Verderio C. 2003. Storage and release of ATP from astrocytes in culture. J Biol Chem 278: 1–354.
6. Crippa D, Schenk U, Francolini M, Rosa P, Verderio C, Zonta M, Pozzan T, Matteoli M, Carmignoto G. 2006. Synaptobrevin2-expressing vesicles in rat astrocytes: Insights into molecular characterization, dynamics and exocytosis. J Physiol 570: 5–67.
7. Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P. 2006. P2Y1 receptor-evoked glutamate exocytosis from astrocytes: Control by tumor necrosis factor-α and prostaglandins. J Biol Chem 41: 3–0684.
8. Jourdain P, Bergersen LH, Bhaukaurally K, Bezzi P, Santello M, Domercq M, Matute C, Tonello F, Gundersen V, Volterra A. 2007. Glutamate exocytosis from astrocytes controls synaptic strength. Nat Neurosci 10: 3–31.
9. 2+-regulated exocytosis from cortical astrocytes. J Neurosci 28: 7–648.
10. Malosio ML, Giordano T, Laslop A, Meldolesi J. 2004. Dense-core granules: A specific hallmark of the neuronal/neurosecretory cell phenotype. J Cell Sci 117: 7–43.
11. 2+ transients control exo-endocytosis of synaptic-like microvesicles in astrocytes. J Neurosci 28: 9–122.
12. Martineau M, Galli T, Baux G, Mothet JP. 2008. Confocal imaging and tracking of the exocytotic routes for D-serinemediated gliotransmission. Glia 56: 1–271.
13. Meldolesi J, Chieregatti E, Luisa Malosio M. 2004. Requirements for the identification of dense-core granules. Trends Cell Biol 14: 1–3.
14. Mothet JP, Pollegioni L, Ouanounou G, Martineau M, Fossier P, Baux G. 2005. Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine. Proc Natl Acad Sci 102: 5–606.
15. Panatier A, Theodosis DT, Mothet JP, Touquet B, Pollegioni L, Poulain DA, Oliet SH. 2006. Glia-derived D-serine controls NMDA receptor activity and synaptic memory. Cell 125: 7–75.
16. Ramamoorthy P, Whim MD. 2008. Trafficking and fusion of neuropeptide Y-containing dense-core granules in astrocytes. J Neurosci 28: 13815–13827.
17. 2+ signalling: Dense core granule exocytosis viewed by total internal reflection fluorescence microscopy. Methods 46: 2–33.
18. 2+ oscillations and cell migration. Glia 55: 6–52.
19. Taupenot L, Harper KL, Mahapatra NR, Parmer RJ, Mahata SK, O’Connor DT. 2002. Identification of a novel sorting determinant for the regulated pathway in the secretory protein chromogranin A. J Cell Sci 115: 4–827.
20. Voglmaier SM, Kam K, Yang H, Fortin DL, Hua Z, Nicoll RA, Edwards RH. 2006. Distinct endocytic pathways control the rate and extent of synaptic vesicle protein recycling. Neuron 1: 7–1.
21. Volterra A, Meldolesi J. 2005. Astrocytes, from brain glue to communication elements: The revolution continues. Nat Rev Neurosci 6: 6–26.
22. Zhang Z, Chen G, Zhou W, Song A, Xu T, Luo Q, Wang W, Gu XS, Duan S. 2007. Regulated ATP release from astrocytes through lysosome exocytosis. Nat Cell Biol 9: 9–45.