Generation and analysis of lentivirus expressing a 2A peptide-linked bicistronic fluorescent construct

Cold Spring Harbor Protocols

Volumn 2014, Issue 12, 2014, Pages 1290-1311

  Huss, David ^a,b   Lansford, Rusty ^a,b  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b CHILDREN S HOSPITAL LOS ANGELES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DENDRA2 FLUORESCENT PROTEIN; DNA POLYMERASE; H2B CERULEAN; LENTIVIRUS VECTOR; PEPTIDES AND PROTEINS; UNCLASSIFIED DRUG; DNA; GREEN FLUORESCENT PROTEIN; OLIGONUCLEOTIDE; PEPTIDE;

2A PEPTIDE LINKED BICISTRONIC FLUORESCENT CONSTRUCT; ANIMAL CELL; ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; ELECTROPHORESIS; EPIFLUORESCENCE MICROSCOPY; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE CONSTRUCT; GENETIC ANALYSIS; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; 3T3 CELL LINE; AGAR GEL ELECTROPHORESIS; ANIMAL; CHICKEN; FLUORESCENCE MICROSCOPY; GENE VECTOR; GENETIC PROCEDURES; GENETIC TRANSFORMATION; GENETICS; HEK293 CELL LINE; LENTIVIRINAE; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

CYTOMEGALOVIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; LENTIVIRUS;

ANIMALS; BASE SEQUENCE; CHICKENS; DNA; ELECTROPHORESIS, AGAR GEL; ESCHERICHIA COLI; GENETIC TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; LENTIVIRUS; MICE; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NIH 3T3 CELLS; OLIGONUCLEOTIDES; PEPTIDES; PLASMIDS; POLYMERASE CHAIN REACTION; TRANSFECTION; TRANSFORMATION, GENETIC;

EID: 84923781513     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot081422     Document Type: Article

References (3)

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2. De Palma M, Venneri MA, Naldini L. 2003. In vivo targeting of tumor endothelial cells by systemic delivery of lentiviral vectors. Hum Gene Ther 14: 1193–1206.
3. Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P. 2004. Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proc Natl Acad Sci 101: 18206–18211.