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Volumn 5, Issue 1, 2015, Pages

A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids

Author keywords

Fusion PCR; Homologous recombination; Molecular tools; Optimization; Tagging; Trypanosomatid

Indexed keywords

LEISHMANIA MEXICANA; TRYPANOSOMA BRUCEI; TRYPANOSOMATIDAE;

EID: 84923675616     PISSN: None     EISSN: 20462441     Source Type: Journal    
DOI: 10.1098/rsob.140197     Document Type: Article
Times cited : (176)

References (32)
  • 2
    • 84855916360 scopus 로고    scopus 로고
    • Expression of multisubunit proteins in Leishmania tarentolae
    • Sugino M, Niimi T. 2012 Expression of multisubunit proteins in Leishmania tarentolae. Methods Mol. Biol. 824, 317-325. (doi:10.1007/978-1-61779-433-9-16)
    • (2012) Methods Mol. Biol. , vol.824 , pp. 317-325
    • Sugino, M.1    Niimi, T.2
  • 3
    • 0028693997 scopus 로고
    • Chapter 4: Transfection experiments with leishmania
    • LeBowitz JH. 1995 Chapter 4: transfection experiments with leishmania. Methods Cell Biol. 45, 65-78. (doi:10.1016/S0091-679X(08)61846-4)
    • (1995) Methods Cell Biol. , vol.45 , pp. 65-78
    • LeBowitz, J.H.1
  • 4
    • 0028989439 scopus 로고
    • Inducible gene expression in trypanosomes mediated by a prokaryotic repressor
    • Wirtz LE, Clayton CE. 1995 Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 268, 1179-1183. (doi:10.1126/science.7761835)
    • (1995) Science , vol.268 , pp. 1179-1183
    • Wirtz, L.E.1    Clayton, C.E.2
  • 5
    • 0033928734 scopus 로고    scopus 로고
    • Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA
    • Shi H, Djikeng A, Mark T, Wirtz LE, Tschudi C, Ullu E. 2000 Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 6, 1069-1076. (doi:10.1017/S1355838 200000297)
    • (2000) RNA , vol.6 , pp. 1069-1076
    • Shi, H.1    Djikeng, A.2    Mark, T.3    Wirtz, L.E.4    Tschudi, C.5    Ullu, E.6
  • 7
    • 84856955769 scopus 로고    scopus 로고
    • High-throughput decoding of antitrypanosomal drug efficacy and resistance
    • Alsford S et al. 2012 High-throughput decoding of antitrypanosomal drug efficacy and resistance. Nature 482, 232-236. (doi:10.1038/nature10771)
    • (2012) Nature , vol.482 , pp. 232-236
    • Alsford, S.1
  • 8
    • 84895900845 scopus 로고    scopus 로고
    • Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei
    • Mony BM, MacGregor P, Ivens A, Rojas F, Cowton A, Young J, Horn D, Matthews K. 2014 Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei. Nature 505, 681-685. (doi:10.1038/nature12864)
    • (2014) Nature , vol.505 , pp. 681-685
    • Mony, B.M.1    MacGregor, P.2    Ivens, A.3    Rojas, F.4    Cowton, A.5    Young, J.6    Horn, D.7    Matthews, K.8
  • 9
    • 33644858832 scopus 로고    scopus 로고
    • Flagellar motility is required for the viability of the bloodstream trypanosome
    • Broadhead R et al. 2006 Flagellar motility is required for the viability of the bloodstream trypanosome. Nature 440, 224-227. (doi:10.1038/ nature04541)
    • (2006) Nature , vol.440 , pp. 224-227
    • Broadhead, R.1
  • 10
    • 84902087104 scopus 로고    scopus 로고
    • High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics
    • Güther MLS, Urbaniak MD, Tavendale A, Prescott A, Ferguson MAJ. 2014 High-confidence glycosome proteome for procyclic form Trypanosoma brucei by epitope-tag organelle enrichment and SILAC proteomics. J. Proteome Res. 13, 2796-2806. (doi:10.1021/pr401209w)
    • (2014) J. Proteome Res. , vol.13 , pp. 2796-2806
    • Güther, M.L.S.1    Urbaniak, M.D.2    Tavendale, A.3    Prescott, A.4    Ferguson, M.A.J.5
  • 13
    • 33846259286 scopus 로고    scopus 로고
    • Analysis of the Leishmania donovani transcriptome reveals an ordered progression of transient and permanent changes in gene expression during differentiation
    • Saxena A, Lahav T, Holland N, Aggarwal G, Anupama A, Huang Y, Volpin H, Myler PJ, Zilberstein D. 2007 Analysis of the Leishmania donovani transcriptome reveals an ordered progression of transient and permanent changes in gene expression during differentiation. Mol. Biochem. Parasitol. 152, 53-65. (doi:10.1016/j. molbiopara.2006.11.011)
    • (2007) Mol. Biochem. Parasitol. , vol.152 , pp. 53-65
    • Saxena, A.1    Lahav, T.2    Holland, N.3    Aggarwal, G.4    Anupama, A.5    Huang, Y.6    Volpin, H.7    Myler, P.J.8    Zilberstein, D.9
  • 14
    • 79953670282 scopus 로고    scopus 로고
    • The cell cycle regulated transcriptome of Trypanosoma brucei
    • Archer SK, Inchaustegui D, Queiroz R, Clayton C. 2011 The cell cycle regulated transcriptome of Trypanosoma brucei. PLoS ONE 6, e18425. (doi:10. 1371/journal.pone.0018425)
    • (2011) PLoS ONE , vol.6 , pp. e18425
    • Archer, S.K.1    Inchaustegui, D.2    Queiroz, R.3    Clayton, C.4
  • 15
    • 77956127684 scopus 로고    scopus 로고
    • Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites
    • Siegel TN, Hekstra DR, Wang X, Dewell S, Cross GAM. 2010 Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites. Nucleic Acids Res. 38, 4946-4957. (doi:10.1093/nar/ gkq237)
    • (2010) Nucleic Acids Res. , vol.38 , pp. 4946-4957
    • Siegel, T.N.1    Hekstra, D.R.2    Wang, X.3    Dewell, S.4    Cross, G.A.M.5
  • 16
    • 77958123318 scopus 로고    scopus 로고
    • Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei
    • Nilsson D, Gunasekera K, Mani J, Osteras M, Farinelli L, Baerlocher L, Roditi I, Ochsenreiter T. 2010 Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei. PLoS Pathog. 6, e1001037. (doi:10.1371/journal.ppat.1001037)
    • (2010) PLoS Pathog. , vol.6 , pp. e1001037
    • Nilsson, D.1    Gunasekera, K.2    Mani, J.3    Osteras, M.4    Farinelli, L.5    Baerlocher, L.6    Roditi, I.7    Ochsenreiter, T.8
  • 17
    • 34249992854 scopus 로고    scopus 로고
    • Functional genomics in Trypanosoma brucei: A collection of vectors for the expression of tagged proteins from endogenous and ectopic gene loci
    • Kelly S et al. 2007 Functional genomics in Trypanosoma brucei: a collection of vectors for the expression of tagged proteins from endogenous and ectopic gene loci. Mol. Biochem. Parasitol. 154, 103-109. (doi:10.1016/j.molbiopara.2007.03.012)
    • (2007) Mol. Biochem. Parasitol. , vol.154 , pp. 103-109
    • Kelly, S.1
  • 18
    • 34250863858 scopus 로고    scopus 로고
    • Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases
    • Barnes RL, Mcculloch R. 2007 Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases. Nucleic Acids Res. 35, 3478-3493. (doi:10.1093/nar/gkm249)
    • (2007) Nucleic Acids Res. , vol.35 , pp. 3478-3493
    • Barnes, R.L.1    Mcculloch, R.2
  • 19
    • 28044439662 scopus 로고    scopus 로고
    • A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genes
    • Oberholzer M, Morand S, Kunz S, Seebeck T. 2006 A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genes. Mol. Biochem. Parasitol. 145, 117-120. (doi:10.1016/j. molbiopara.2005.09.002)
    • (2006) Mol. Biochem. Parasitol. , vol.145 , pp. 117-120
    • Oberholzer, M.1    Morand, S.2    Kunz, S.3    Seebeck, T.4
  • 20
    • 3242685458 scopus 로고    scopus 로고
    • A PCR-based method for gene deletion and protein tagging in Trypanosoma brucei
    • Arhin GK, Shen S, Ullu E, Tschudi C. 2004 A PCR-based method for gene deletion and protein tagging in Trypanosoma brucei. Methods Mol. Biol. 270, 277-286. (doi:10.1385/1-59259-793-9:277)
    • (2004) Methods Mol. Biol. , vol.270 , pp. 277-286
    • Arhin, G.K.1    Shen, S.2    Ullu, E.3    Tschudi, C.4
  • 21
    • 66149125717 scopus 로고    scopus 로고
    • Regulated expression of the Leishmania major surface virulence factor lipophosphoglycan using conditionally destabilized fusion proteins
    • Madeira da Silva L, Owens KL, Murta SMF, Beverley SM. 2009 Regulated expression of the Leishmania major surface virulence factor lipophosphoglycan using conditionally destabilized fusion proteins. Proc. Natl Acad. Sci. USA 106, 7583-7588. (doi:10. 1073/pnas.0901698106)
    • (2009) Proc. Natl Acad. Sci. USA , vol.106 , pp. 7583-7588
    • Madeira Da Silva, L.1    Owens, K.L.2    Murta, S.M.F.3    Beverley, S.M.4
  • 22
    • 0025650360 scopus 로고
    • Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes
    • LeBowitz JH, Coburn CM, McMahon-Pratt D, Beverley SM. 1990 Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proc. Natl Acad. Sci. USA 87, 9736-9740. (doi:10.1073/pnas. 87.24.9736)
    • (1990) Proc. Natl Acad. Sci. USA , vol.87 , pp. 9736-9740
    • LeBowitz, J.H.1    Coburn, C.M.2    McMahon-Pratt, D.3    Beverley, S.M.4
  • 23
    • 0030665360 scopus 로고    scopus 로고
    • Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania
    • Papadopoulou B, Dumas C. 1997 Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania. Nucleic Acids Res. 25, 4278-4286. (doi:10.1093/nar/25.21.4278)
    • (1997) Nucleic Acids Res. , vol.25 , pp. 4278-4286
    • Papadopoulou, B.1    Dumas, C.2
  • 24
    • 0029894292 scopus 로고    scopus 로고
    • A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei
    • Bastin P, Bagherzadeh Z, Matthews KR, Gull K. 1996 A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei. Mol. Biochem. Parasitol. 77, 235-239. (doi:10.1016/0166-6851(96)02598-4)
    • (1996) Mol. Biochem. Parasitol. , vol.77 , pp. 235-239
    • Bastin, P.1    Bagherzadeh, Z.2    Matthews, K.R.3    Gull, K.4
  • 25
    • 34247480578 scopus 로고    scopus 로고
    • Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei
    • Burkard G, Fragoso CM, Roditi I. 2007 Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei. Mol. Biochem. Parasitol. 153, 220-223. (doi:10.1016/j.molbiopara.2007. 02.008)
    • (2007) Mol. Biochem. Parasitol. , vol.153 , pp. 220-223
    • Burkard, G.1    Fragoso, C.M.2    Roditi, I.3
  • 26
    • 0025122367 scopus 로고
    • Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression
    • Kapler GM, Coburn CM, Beverley SM. 1990 Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression. Mol. Cell Biol. 10, 1084-1094. (doi:10.1128/MCB.10.3.1084)
    • (1990) Mol. Cell Biol. , vol.10 , pp. 1084-1094
    • Kapler, G.M.1    Coburn, C.M.2    Beverley, S.M.3
  • 27
    • 78751700188 scopus 로고    scopus 로고
    • The cell cycle of Leishmania: Morphogenetic events and their implications for parasite biology
    • Wheeler RJ, Gluenz E, Gull K. 2011 The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology. Mol. Microbiol. 79, 647-662. (doi:10.1111/j.1365-2958.2010. 07479.x)
    • (2011) Mol. Microbiol. , vol.79 , pp. 647-662
    • Wheeler, R.J.1    Gluenz, E.2    Gull, K.3
  • 28
    • 80055106263 scopus 로고    scopus 로고
    • Morphological events during the cell cycle of Leishmania major
    • Ambit A, Woods KL, Cull B, Coombs GH, Mottram JC. 2011 Morphological events during the cell cycle of Leishmania major. Eukaryotic Cell 10, 1429-1438. (doi:10.1128/EC.05118-11)
    • (2011) Eukaryotic Cell , vol.10 , pp. 1429-1438
    • Ambit, A.1    Woods, K.L.2    Cull, B.3    Coombs, G.H.4    Mottram, J.C.5
  • 29
    • 75549088870 scopus 로고    scopus 로고
    • TriTrypDB: A functional genomic resource for the Trypanosomatidae
    • Aslett M et al. 2010 TriTrypDB: a functional genomic resource for the Trypanosomatidae. Nucleic Acids Res. 38, D457-D462. (doi:10.1093/nar/ gkp851)
    • (2010) Nucleic Acids Res. , vol.38 , pp. D457-D462
    • Aslett, M.1
  • 30
    • 84876557849 scopus 로고    scopus 로고
    • EuPathDB: The eukaryotic pathogen database
    • Aurrecoechea C et al. 2013 EuPathDB: the eukaryotic pathogen database. Nucleic Acids Res. 41, D684-D691. (doi:10.1093/nar/gks1113)
    • (2013) Nucleic Acids Res. , vol.41 , pp. D684-D691
    • Aurrecoechea, C.1
  • 31
    • 84886100620 scopus 로고    scopus 로고
    • Identification of essential and non-essential protein kinases by a fusion PCR method for efficient production of transgenic Trypanosoma brucei
    • Merritt C, Stuart K. 2013 Identification of essential and non-essential protein kinases by a fusion PCR method for efficient production of transgenic Trypanosoma brucei. Mol. Biochem. Parasitol. 190, 44-49. (doi:10.1016/j.molbiopara.2013.05.002)
    • (2013) Mol. Biochem. Parasitol. , vol.190 , pp. 44-49
    • Merritt, C.1    Stuart, K.2
  • 32
    • 84864199562 scopus 로고    scopus 로고
    • A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor
    • Poon SK, Peacock L, Gibson W, Gull K, Kelly S. 2012 A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor. Open Biol. 2, 110037. (doi:10.1098/rsob.110037)
    • (2012) Open Biol. , vol.2 , pp. 110037
    • Poon, S.K.1    Peacock, L.2    Gibson, W.3    Gull, K.4    Kelly, S.5


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