Reconstitution of a transmembrane protein, the voltage-gated ion channel, KvAP, into giant unilamellar vesicles for microscopy and patch clamp studies

Journal of Visualized Experiments

Volumn , Issue 95, 2015, Pages

  Garten, Matthias ^a   Aimon, Sophie ^b   Bassereau, Patricia ^a   Toombes, Gilman E S ^c  

^a INSTITUT CURIE   (France)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

Author keywords

Agarose; Biochemistry; Biomimetic model system; Electroformation; Electrophysiology; Fluorescence microscopy; Gel assisted swelling; Giant Unilamellar Vesicle; Inside out patch clamp; Ion channel; Issue 95; KvAP; Reconstitution; Transmembrane protein

Indexed keywords

LIPOSOME; VOLTAGE GATED POTASSIUM CHANNEL;

CHEMISTRY; FLUORESCENCE MICROSCOPY; METABOLISM; PATCH CLAMP TECHNIQUE; PROCEDURES;

MICROSCOPY, FLUORESCENCE; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNELS, VOLTAGE-GATED; UNILAMELLAR LIPOSOMES;

EID: 84923629018     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/52281     Document Type: Article

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