An introduction to dengue-disease diagnostics

TrAC - Trends in Analytical Chemistry

Volumn 67, Issue , 2015, Pages 45-55

  Darwish, Nadiya Taha ^a   Alias, Yatimah Binti ^a   Khor, Sook Mei ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Biomarker; Biosensor; Capture IgM; Dengue NS1 antigen; Dengue virus; Diagnosis; IgG ELISA; Immunochromatography; Real time polymerase chain reaction; Virus isolation

Indexed keywords

ANTIGENS; BIOMARKERS; BIOMATERIALS; BIOSENSORS; DIAGNOSIS; VIRUSES;

CAPTURE IGM; DENGUE VIRUS; IGG ELISA; IMMUNOCHROMATOGRAPHY; REAL TIME POLYMERASE CHAIN REACTIONS;

POLYMERASE CHAIN REACTION;

ANTIGEN; BIOLOGICAL MARKER; CHLORPHENOTANE; ENVELOPE PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; NS1 ANTIGEN; UNCLASSIFIED DRUG;

ANTIBODY TITER; ANTIGEN DETECTION; BIOSENSOR; DENGUE; DENGUE VIRUS; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC TEST ACCURACY STUDY; DIFFERENTIAL DIAGNOSIS; DIFFERENTIAL PULSE VOLTAMMETRY; DNA PROBE; ELECTROCHEMICAL DETECTION; ELECTRODE; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIDEMIC; FEVER; GENETIC SCREENING; GENOMICS; GENOSENSOR; GEOGRAPHIC DISTRIBUTION; GLASSY CARBON ELECTRODE; HUMAN; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOSENSOR; LIMIT OF DETECTION; MEDICAL HISTORY; MICROFLUIDIC ANALYSIS; MORTALITY; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; OPTICAL BIOSENSOR; PANDEMIC; PENCIL LEAD GRAPHITE; PIEZOELECTRICITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVIEW; SENSITIVITY AND SPECIFICITY; SEROLOGY; SYMPTOMATOLOGY; VIREMIA; VIRUS DETECTION; VIRUS ISOLATION; VIRUS PARTICLE; VIRUS TRANSMISSION;

EID: 84923296432     PISSN: 01659936     EISSN: 18793142     Source Type: Journal    
DOI: 10.1016/j.trac.2015.01.005     Document Type: Review

References (94)

1. Bessaud M., Pastorino B.A.M., Peyrefitte C.N., Rolland D., Grandadam M., Tolou H.J. Functional characterization of the NS2B/NS3 protease complex from seven viruses belonging to different groups inside the genus Flavivirus. Virus Res 2006, 120:79-90.
2. Lim S.P., Wang Q.-Y., Noble C.G., Chen Y.-L., Dong H., Zou B., et al. Ten years of dengue drug discovery: progress and prospects. Antiviral Res 2013, 100:500-519.
3. Bhakat S., Karubiu W., Jayaprakash V., Soliman M.E.S. A perspective on targeting non-structural proteins to combat neglected tropical diseases: dengue, west nile and chikungunya viruses. Eur. J. Med. Chem 2014, 87:677-702.
4. Ashley E.A. Dengue fever. Trends Anaesth. Crit. Care 2011, 1:39-41.
5. Decker K.D. Dengue fever: re-emergence of an old virus. J. Nurse Pract 2012, 8:389-393.
6. Dale Carroll I., Toovey S., Gompel A.V. Dengue fever and pregnancy- a review and comment. Travel Med. Infect. Dis 2007, 5:183-188.
7. Bakshi A.S. Dengue fever, DHF and DSS. Apollo Med 2007, 4:111-117.
8. Guzman M.G., Kouri G. Dengue and dengue hemorrhagic fever in the Americas: lessons and challenges. J. Clin. Virol 2003, 27:1-13.
9. Chen R.-F., Yang K.D., Wang L., Liu J.-W., Chiu C.-C., Cheng J.-T. Different clinical and laboratory manifestations between dengue haemorrhagic fever and dengue fever with bleeding tendency. Trans. R. Soc. Trop. Med. Hyg 2007, 101:1106-1113.
10. Wright W.F., Pritt B.S. Update: the diagnosis and management of dengue virus infection in North America. Diagn. Microbiol. Infect. Dis 2012, 73:215-220.
11. Yamada K.-I., Takasaki T., Nawa M., Kurane I. Virus isolation as one of the diagnostic methods for dengue virus infection. J. Clin. Virol 2002, 24:203-209.
12. Henchal E.A., McCown J.M., Seguin M.C. Rapid identification of dengue virus isolates by using monoclonal antibodies in an indirect immunofluorescent assay. Am. J. Trop. Med. Hyg 1983, 32:164-169.
13. Suwandono A., Kosasih H., Nurhayati, Kusriastuti R., Harun S., Maaroef C., et al. Four dengue virus serotypes found circulating during an outbreak of dengue fever and dengue haemorrhagic fever in Jakarta, Indonesia, during 2004. Trans. R. Soc. Trop. Med. Hyg 2006, 100:855-862.
14. Kumaria R., Chakravarti A. Molecular detection and serotypic characterization of dengue viruses by single-tube multiplex reverse transcriptase- polymerase chain reaction. Diagn. Microbiol. Infect. Dis 2005, 52:311-316.
15. Raengsakulrach B., Nisalak A., Maneekarn N., Yenchitsomanus P.-T., Limsomwong C., Jairungsri A., et al. Comparison of four reverse transcription-polymerase chain reaction procedures for the detection of dengue virus in clinical specimens. J. Virol. Methods 2002, 105:219-232.
16. Dash P.K., Parida M., Santhosh S.R., Saxena P., Srivastava A., Neeraja M., et al. Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection. Diagn. Microbiol. Infect. Dis 2008, 62:52-57.
17. Deubel V., Laille M., Hugnot J.P., Chungue E., Guesdon J.L., Drouet M.T., et al. Identification of dengue sequences by genomic amplification: rapid diagnosis of dengue virus serotypes in peripheral blood. J. Virol. Methods 1990, 30:41-54.
18. Morita K., Tanaka M., Igarashi A. Rapid identification of dengue virus serotypes by using polymerase chain reactio. J. Clin. Microbiol 1991, 29:2107-2110.
19. Lanciotti R.S., Calisher C.H., Gubler D.J., Chang G.J., Vorndam A.V. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J. Clin. Microbiol 1992, 30:545-551.
20. Chang G.J.J., Trent D.W., Vorndam A.V., Vergne E., Kinney R.M., Mitchell C.J. An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses. J. Clin. Microbiol 1994, 32:477-483.
21. Seah C.L.K., Chow V.T.K., Chan Y.C. Semi-nested PCR using NS3 primers for the detection and typing of dengue viruses in clinical serum specimens. J. Clin. Virol 1995, 4:113-120.
22. Yenchitsomanus P.T., Sricharoen P., Jaruthasana I., Pattanakitsakul S.N., Nitayaphan S., Mongkolsapaya J., et al. Rapid detection and identification of dengue viruses by polymerase chain reaction (PCR). Southeast Asian J. Trop. Med. Public Health 1996, 27:228-236.
23. Meiyu F., Huosheng C., Cuihua C., Xiaodong T., Lianhua J., Yifei P., et al. Detection of Flaviviruses by reverse transcriptase-polymerase chain reaction with the universal primer set. Microbiol. Immunol 1997, 41:209-213.
24. Sudiro T.M., Ishiko H., Rothman A.L., Kershaw D.E., Green S., Vaughn D.W., et al. Microplate-reverse hybridization method to determine dengue virus serotype. J. Virol. Methods 1998, 73:229-235.
25. Harris E., Roberts T.G., Smith L., Selle J., Kramer L.D., Valle S., et al. Typing of dengue viruses in clinical specimens and mosquitoes by single- tube multiplex reverse transcriptase PCR. J. Clin. Microbiol 1998, 36:2634-2639.
26. Laue T., Emmerich P., Schmitz H. Detection of dengue virus RNA in patients after primary or secondary dengue infection by using the TaqMan automated amplification system. J. Clin. Microbiol 1999, 37:2543-2547.
27. Houng H.S.H., Chung-Ming Chen R., Vaughn D.W., Kanesa-thasan N. Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype-specific noncoding sequences. J. Virol. Methods 2001, 95:19-32.
28. De Paula S.O., de Melo Lima C., Torres M.P., Pereira M.R.G., Lopes da Fonseca B.A. One-step RT-PCR protocols improve the rate of dengue diagnosis compared to two-step RT-PCR approaches. J. Clin. Virol 2004, 30:297-301.
29. Hue K.D.T., Tuan T.V., Thi H.T.N., Bich C.T.N., Anh H.H.L., Wills B.A., et al. Validation of an internally controlled one-step real-time multiplex RT-PCR assay for the detection and quantitation of dengue virus RNA in plasma. J. Virol. Methods 2011, 177:168-173.
30. Rosen L., Khin M.M., Tin U. Recovery of virus from the liver of children with fatal dengue: reflections on the pathogenesis of the disease and its possible analogy with that of yellow fever. Res. Virol 1989, 140:351-360.
31. Muller D.A., Frentiu F.D., Rojas A., Moreira L.A., O-Neill S.L., Young P.R. A portable approach for the surveillance of dengue virus-infected mosquitoes. J. Virol. Methods 2010, 183:90-93.
32. Liew K.J.L., Chow V.T.K. Microarray and real-time RT-PCR analyses of a novel set of differentially expressed human genes in ECV304 endothelial-like cells infected with dengue virus type 2. J. Virol. Methods 2006, 131:47-57.
33. Tahir M., Gupta E., Salmani S., Padma M.V., Singh M.B., Dar L., et al. Dengue fever with papilledema: a case of dengue-3 virus infection in central nervous system. J. Clin. Virol 2006, 37:65-67.
34. Mizuno Y., Kotaki A., Harada F., Tajima S., Kurane I., Takasaki T. Confirmation of dengue virus infection by detection of dengue virus type 1 genome in urine and saliva but not in plasma. Trans. R. Soc. Trop. Med. Hyg 2007, 101:738-739.
35. Prado I., Rosario D., Bernardo L., Alvarez M., Rodríguez R., Vasquez S., Guzman M.G. PCR detection of dengue virus using dried whole blood spotted on filter paper. J. Virol. Methods 2005, 125:75-81.
36. Guzman M.G., Kouri G. Dengue diagnosis, advances and challenges. Int. J. Infect. Dis 2004, 8:69-80.
37. Houng H.-S.H., Chung-Ming Chen R., Vaughn D.W., Kanesa-thasan N. Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype-specific 3- noncoding sequences. J. Virol. Methods 2001, 95:19-32.
38. Lemmer K., Donoso Mantke O., Bae H.-G., Groen J., Drosten C., Niedrig M. External quality control assessment in PCR diagnostics of dengue virus infections. J. Clin. Virol 2004, 30:291-296.
39. Barkham T.M., Chung Y.K., Tang K.F., Ooi E.E. The performance of RT-PCR compared with a rapid serological assay for acute dengue fever in a diagnostic laboratory. Trans. R. Soc. Trop. Med. Hyg 2006, 100:142-148.
40. Raggi C.C., Pinzani P., Paradiso A., Pazzagli M., Orlando C. External qualitiy assurance program for PCR amplification of genomic DNA: an Italian experience. Clin. Chem 2003, 49:72-91.
41. Anez M., Putonti C., Fox G.E., Fofanov Y., Willson R.C. Exhaustive computational identification of pathogen sequences far-distant from background genomes: identification and experimental verification of human-blind dengue PCR primers. J. Biotechnol 2008, 133:267-276.
42. Rivetz B., Siman-Tov D., Ambal E., Jaramillo A.-C., Ben-Zvi A., Tartakovsky B., et al. New dengue antibody assay with unique differential detection of IgG and IgM antibodies. Clin. Biochem 2009, 42:180-184.
43. Vazquez S., Valdes O., Pupo M., Delgado I., Alvarez M., Pelegrino J.L., et al. MAC-ELISA and ELISA inhibition methods for detection of antibodies after yellow fever vaccination. J. Virol. Methods 2003, 110:179-184.
44. Nuegoonpipat A.A., Panthuyosri N., Anantapreecha S., Chanama S., Sa-Ngasang A., Sawanpanyalert P., et al. Cross-reactive IgM responses in patients with dengue or Japanese encephalitis. J. Clin. Virol 2008, 42:75-77.
45. Guzman M., Meschino G.J., Dai Pra A.L., Trivi M., Passoni L.I., Rabal H. Dynamic laser speckle: decision models with computational intelligence techniques 2010, Proc. SPIE 7387, Speckle: Optical Metrology, 738717.
46. Huang C.-H., Kuo L.-L., Yang K.D., Lin P.-S., Lu P.-L., Lin C.-C., et al. Laboratory diagnostics of dengue fever: an emphasis on the role of commercial dengue virus nonstructural protein 1 antigen rapid test. J. Microbiol. Immunol. Infect 2013, 46:358-365.
47. Shenoy B., Menon A., Biradar S. Diagnostic utility of dengue NS1 antigen. Pediatr. Infect. Dis 2014, 6:110-113.
48. Watthanaworawit W., Turner P., Turner C.L., Tanganuchitcharnchai A., Jarman R.G., Blacksell S.D., et al. A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border. Trans. R. Soc. Trop. Med. Hyg 2011, 105:32-37.
49. Teles F.S. Biosensors and rapid diagnostic tests on the frontier between analytical and clinical chemistry for biomolecular diagnosis of dengue disease: a review. Anal. Chim. Acta 2011, 687:28-42.
50. Zhang H., Li W., Wang J., Peng H., Che X., Chen X., et al. NS1-based tests with diagnostic utility for confirming dengue infection: a meta-analysis. Int. J. Infect. Dis 2014, 26:57-66.
51. Kryscio D.R., Peppas N.A. Critical review and perspective of macromolecularly imprinted polymers. Acta Biomater 2012, 8:461-473.
52. Chen S.H., Chuang Y.C., Lu Y.C., Lin H.C., Yang Y.L., Lin C.S. A method of layer-by-layer gold nanoparticle hybridization in a quartz crystal microbalance DNA sensing system used to detect dengue virus. Nanotechnology 2009, 20:215501.
53. Su C.-C., Wu T.-Z., Chen L.-K., Yang H.-H., Tai D.-F. Development of immunochips for the detection of dengue viral antigens. Anal. Chim. Acta 2003, 479:117-123.
54. Wu T.-Z., Su C.-C., Chen L.-K., Yang H.-H., Tai D.-F., Peng K.-C. Piezoelectric immunochip for the detection of dengue fever in viremia phase. Biosens. Bioelectron 2005, 21:689-695.
55. Tai D.F., Lin C.Y., Wu T.Z., Huang J.H., Shu P.Y. Artificial receptors in serologic tests for the early diagnosis of dengue virus infection. Clin. Chem 2006, 52:1486-1491.
56. Baeumner A.J., Schlesinger N.A., Slutzki N.S., Romano J., Lee E.M., Montagna R.A. Biosensor for dengue virus detection: sensitive, rapid, and serotype specific. Anal. Chem 2002, 74:1442-1448.
57. Zaytseva N.V., Montagna R.A., Baeumner A.J. Microfluidic biosensor for the serotype-specific detection of dengue virus RNA. Anal. Chem 2005, 77:7520-7527.
58. Fletcher S.J., Phillips L.W., Milligan A.S., Rodda S.J. Toward specific detection of dengue virus serotypes using a novel modular biosensor. Biosens. Bioelectron 2010, 26:1696-1700.
59. Atias D., Liebes Y., Chalifa-Caspi V., Bremand L., Lobel L., Marks R.S., et al. Chemiluminescent optical fiber immunosensor for the detection of IgM antibody to dengue virus in humans. Sens. Actuators B Chem 2009, 140:206-215.
60. Lee Y.F., Lien K.Y., Lei H.Y., Lee G.B. An integrated microfluidic system for rapid diagnosis of dengue virus infection. Biosens. Bioelectron 2009, 25:745-752.
61. Kumbhat S., Sharma K., Gehlot R., Solanki A., Joshi V. Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection. J. Pharm. Biomed. Anal 2010, 52:255-259.
62. Melendez J., Carr R., Bartholomew D., Taneja H., Yee S., Jung C., et al. Development of a surface plasmon resonance sensor for commercial applications. Sens. Actuators B Chem 1997, 39:375-379.
63. Chen W.-H., Hsu I.H., Sun Y.-C., Wang Y.-K., Wu T.-K. Immunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virus. J. Chromatogr. A 2013, 1288:21-27.
64. Oliveira M.D.L., Correia M.T.S., Diniz F.B. A novel approach to classify serum glycoproteins from patients infected by dengue using electrochemical impedance spectroscopy analysis. Synth. Met 2009, 159:2162-2164.
65. Oliveira M.D.L., Correia M.T.S., Diniz F.B. Concanavalin A and polyvinyl butyral use as a potential dengue electrochemical biosensor. Biosens. Bioelectron 2009, 25:728-732.
66. Andrade C.A.S., Oliveira M.D.L., de Melo C.P., Coelho L.C.B.B., Correia M.T.S., Nogueira M.L., et al. Diagnosis of dengue infection using a modified gold electrode with hybrid organica-inorganic nanocomposite and Bauhinia monandra lectin. J. Colloid Interface Sci 2011, 362:517-523.
67. Oliveira M.D.L., Nogueira M.L., Correia M.T.S., Coelho L.C.B.B., Andrade C.A.S. Detection of dengue virus serotypes on the surface of gold electrode based on cratylia mollis lectin affinity. Sens. Actuators B Chem 2011, 155:789-795.
68. Dias A.C.M.S., Gomes-Filho S.L.R., Silva M.M.S., Dutra R.F. A sensor tip based on carbon nanotube-ink printed electrode for the dengue virus NS1 protein. Biosens. Bioelectron 2013, 44:216-221.
69. Gole A., Dash C., Ramakrishnan V., Sainkar S.R., Mandale A.B., Rao M., et al. Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir 2001, 17:1674-1679.
70. Wang J., Luck L.A., Suni I.I. Immobilization of the glucose-galactose receptor protein onto a Au electrode through a genetically engineered cysteine residue. Electrochem. Solid-State Lett 2007, 10:J33-J36.
71. Kwakye S., Goral V.N., Baeumner A.J. Electrochemical microfluidic biosensor for nucleic acid detection with integrated minipotentiostat. Biosens. Bioelectron 2006, 21:2217-2223.
72. Rivet C., Lee H., Hirsch A., Hamilton S., Lu H. Microfluidics for medical diagnostics and biosensors. Chem. Eng. Sci 2011, 66:1490-1507.
73. Teles F.R.R., Dos Prazeres D.M.F., De Lima-Filho J.L. Electrochemical detection of a dengue-related oligonucleotide sequence using ferrocenium as a hybridization indicator. Sensors 2007, 7:2510-2518.
74. Martins De Souza E., Nascimento G.A., Santana N.A., Bruneska D., Lima Filho J.L. Development of electrochemical biosensor for virus dengue diagnostic using graphite electrode. New Biotechnol 2009, 25:S378.
75. Iqbal S.S., Mayo M.W., Bruno J.G., Bronk B.V., Batt C.A., Chambers J.P. A review of molecular recognition technologies for detection of biological threat agents. Biosens. Bioelectron 2000, 15:549-578.
76. Nascimento H.P.O., Oliveira M.D.L., de Melo C.P., Silva G.J.L., Cordeiro M.T., Andrade C.A.S. An impedimetric biosensor for detection of dengue serotype at picomolar concentration based on gold nanoparticles-polyaniline hybrid composites. Colloids Surf. B. Biointerfaces 2011, 86:414-419.
77. Cheng M.S., Ho J.S., Tan C.H., Wong J.P.S., Ng L.C., Toh C.-S. Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. Anal. Chim. Acta 2012, 725:74-80.
78. Nguyen B.T.T., Peh A.E.K., Chee C.Y.L., Fink K., Chow V.T.K., Ng M.M.L., et al. Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensor. Bioelectrochemistry 2012, 88:15-21.
79. Cavalcanti I.T., Silva B.V.M., Peres N.G., Moura P., Sotomayor M.D.P.T., Guedes M.I.F., et al. A disposable chitosan-modified carbon fiber electrode for dengue virus envelope protein detection. Talanta 2012, 91:41-46.
80. Baeumner A.J., Humiston M.C., Montagna R.A., Durst R.A. Detection of viable oocysts of Cryptosporidium parvum following nucleic acid sequence based amplification. Anal. Chem 2001, 73:1176-1180.
81. Wu S.J., Lee E.M., Putvatana R., Shurtliff R.N., Porter K.R., Suharyono W., et al. Detection of dengue viral RNA using a nucleic acid sequence-based amplification assay. J. Clin. Microbiol 2001, 39:2794-2798.
82. McBride W.J.H. Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn. Microbiol. Infect. Dis 2009, 64:31-36.
83. Houde A., Leblanc D., Poitras E., Ward P., Brassard J., Simard C., et al. Comparative evaluation of RT-PCR, nucleic acid sequence-based amplification (NASBA) and real-time RT-PCR for detection of noroviruses in faecal material. J. Virol. Methods 2006, 135:163-172.
84. Mairhofer J., Roppert K., Ertl P. Microfluidic systems for pathogen sensing: a review. Sensors 2009, 9:4804-4823.
85. Zhang G.-J., Zhang L., Huang M.J., Luo Z.H.H., Tay G.K.I., Lim E.-J.A., et al. Silicon nanowire biosensor for highly sensitive and rapid detection of dengue virus. Sens. Actuators B Chem 2010, 146:138-144.
86. Turner G.A. N-glycosylation of serum proteins in disease and its investigation using lectins. Clin. Chim. Acta 1992, 208:149-171.
87. CDC Laboratory guidance and diagnostic testing Available from:, accessed 28.6.12. http://www.cdc.gov/dengue/clinicalLab/laboratory.html.
88. Alcon S., Talarmin A., Debruyne M., Falconar A., Deubel V., Flamand M. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J. Clin. Microbiol 2002, 40:376-381.
89. Kumarasamy V., Wahab A.H.A., Chua S.K., Hassan Z., Chem Y.K., Mohamad M., et al. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J. Virol. Methods 2007, 140:75-79.
90. Singh M.P., Majumdar M., Singh G., Goyal K., Preet K., Sarwal A., et al. NS1 antigen as an early diagnostic marker in dengue: report from India. Diagn. Microbiol. Infect. Dis 2010, 68:50-54.
91. Xu H., Di B., Pan Y.X., Qiu L.W., Wang Y.D., Hao W., et al. Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: implications for early diagnosis and serotyping of dengue virus infections. J. Clin. Microbiol 2006, 44:2872-2878.
92. Libraty D.H., Young P.R., Pickering D., Endy T.P., Kalayanarooj S., Green S., et al. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J. Infect. Dis 2002, 186:1165-1168.
93. Young P.R., Hilditch P.A., Bletchly C., Halloran W. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J. Clin. Microbiol 2000, 38:1053-1057.
94. Huhtamo E., Hasu E., Uzcátegui N.Y., Erra E., Nikkari S., Kantele A., et al. Early diagnosis of dengue in travelers: comparison of a novel real-time RT-PCR, NS1 antigen detection and serology. J. Clin. Virol 2010, 47:49-53.