Tail proteins of phage T5: Investigation of the effect of the His6-tag position, from expression to crystallisation

Protein Expression and Purification

Volumn 109, Issue , 2015, Pages 70-78

  Noirclerc Savoye, Marjolaine ^b   Flayhan, Ali ^b   Pereira, Cindy ^b   Gallet, Benoit ^b   Gans, Pierre ^b   Ebel, Christine ^b   Breyton, Cécile ^a  

^a INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^b CEA GRENOBLE   (France)

Author keywords

Bacteriophage; Crystallisation; His tag position; Protein purification

Indexed keywords

BACTERIA (MICROORGANISMS);

HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; OLIGOPEPTIDE; VIRAL PROTEIN;

BACTERIOPHAGE; CRYSTALLIZATION; FLUORESCENCE; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SIZE EXCLUSION CHROMATOGRAPHY; SOLUBILITY; ULTRASTRUCTURE;

BACTERIOPHAGES; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CRYSTALLIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENCE; HISTIDINE; MAGNETIC RESONANCE SPECTROSCOPY; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS; SOLUBILITY; VIRAL PROTEINS;

EID: 84923233575     PISSN: 10465928     EISSN: 10960279     Source Type: Journal    
DOI: 10.1016/j.pep.2015.02.003     Document Type: Article

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