High Content Image-Based Screening of a Protease Inhibitor Library Reveals Compounds Broadly Active against Rift Valley Fever Virus and Other Highly Pathogenic RNA Viruses

PLoS Neglected Tropical Diseases

Volumn 8, Issue 8, 2014, Pages

  Mudhasani, Rajini ^a   Kota, Krishna P ^a   Retterer, Cary ^a   Tran, Julie P ^a   Whitehouse, Chris A ^a   Bavari, Sina ^a  

^a UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA2A INTERFERON; BREFELDIN A; C795 0925; D011 2120; F694 1532; G202 0362; GLYCOPROTEIN GC; GLYCOPROTEIN GN; NOCODAZOLE; PROTEINASE INHIBITOR; RIBAVIRIN; TUBULIN; UNCLASSIFIED DRUG; VIRUS GLYCOPROTEIN; MOLECULAR LIBRARY;

ANIMAL CELL; ANTIVIRAL ACTIVITY; ARTICLE; CELL LINE; CHEMICAL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOTOXICITY; DOSE RESPONSE; DRUG MECHANISM; DRUG SCREENING; EBOLA HEMORRHAGIC FEVER; GENE EXPRESSION; GOLGI COMPLEX; HIGH CONTENT IMAGE ANALYSIS; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; LASSA FEVER; MARBURG HEMORRHAGIC FEVER; MICROTUBULE ASSEMBLY; MITOSIS INHIBITION; NONHUMAN; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIFT VALLEY FEVER VIRUS; RIFT VALLEY FEVER VIRUS INFECTION; RNA VIRUS INFECTION; TIME OF ADDITION ASSAY; VENEZUELAN EQUINE ENCEPHALITIS; VIRUS INFECTION; VIRUS RELEASE; VIRUS REPLICATION; WESTERN BLOTTING; ANIMAL; CHLOROCEBUS AETHIOPS; DRUG EFFECTS; EBOLAVIRUS; HELA CELL LINE; IMAGE PROCESSING; MOLECULAR LIBRARY; PROCEDURES; RIFT VALLEY FEVER; VERO CELL LINE; VIROLOGY;

ANIMALS; CERCOPITHECUS AETHIOPS; EBOLAVIRUS; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; PROTEASE INHIBITORS; RIFT VALLEY FEVER; RIFT VALLEY FEVER VIRUS; SMALL MOLECULE LIBRARIES; VERO CELLS; VIROLOGY;

EID: 84922785429     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0003095     Document Type: Article

References (37)

1. Balkhy HH, Memish ZA, (2003) Rift Valley fever: an uninvited zoonosis in the Arabian peninsula. Int J Antimicrob Agents 21: 153–157.
2. Daubney R, Hudson JR, Garnham PC, (1931) Enzootic hepatitis or rift valley fever. An undescribed virus disease of sheep cattle and man from east africa. The Journal of Pathology and Bacteriology 34: 545–579.
3. Laughlin LW, Meegan JM, Strausbaugh LJ, Morens DM, Watten RH, (1979) Epidemic Rift Valley fever in Egypt: observations of the spectrum of human illness. Trans R Soc Trop Med Hyg 73: 630–633.
4. Madani TA, Al-Mazrou YY, Al-Jeffri MH, Mishkhas AA, Al-Rabeah AM, et al. (2003) Rift Valley fever epidemic in Saudi Arabia: epidemiological, clinical, and laboratory characteristics. Clin Infect Dis 37: 1084–1092.
5. Brown JL, Dominik JW, Morrissey RL, (1981) Respiratory infectivity of a recently isolated Egyptian strain of Rift Valley fever virus. Infect Immun 33: 848–853.
6. Patrican LA, Bailey CL, (1989) Ingestion of immune bloodmeals and infection of Aedes fowleri, Aedes mcintoshi, and Culex pipiens with Rift Valley fever virus. Am J Trop Med Hyg 40: 534–540.
7. Bray M, Murphy FA, (2007) Filovirus research: knowledge expands to meet a growing threat. J Infect Dis 196 Suppl 2: S438–443.
8. Yun NE, Walker DH, (2012) Pathogenesis of Lassa fever. Viruses 4: 2031–2048.
9. Quiroz E, Aguilar PV, Cisneros J, Tesh RB, Weaver SC, (2009) Venezuelan equine encephalitis in Panama: fatal endemic disease and genetic diversity of etiologic viral strains. PLoS Negl Trop Dis 3: e472.
10. Bickle M, (2010) The beautiful cell: high-content screening in drug discovery. Anal Bioanal Chem 398: 219–226.
11. Struthers JK, Swanepoel R, Shepherd SP, (1984) Protein synthesis in Rift Valley fever virus-infected cells. Virology 134: 118–124.
12. de Boer SM, Kortekaas J, Spel L, Rottier PJ, Moormann RJ, et al. (2012) Acid-activated structural reorganization of the Rift Valley fever virus Gc fusion protein. J Virol 86: 13642–13652.
13. Pettersson RF, Melin L (1996) Synthesis, assembly, and intracellular transport of Bunyaviridae membrane proteins. In: Elliott RM, editor. The Bunyaviridae. New York, NY: Plenum Press pp. 159–188.
14. Carnec X, Ermonval M, Kreher F, Flamand M, Bouloy M, (2014) Role of the cytosolic tails of Rift Valley fever virus envelope glycoproteins in viral morphogenesis. Virology 448: 1–14.
15. Caplen H, Peters CJ, Bishop DH, (1985) Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development. J Gen Virol 66(Pt 10): 2271–2277.
16. Habjan M, Pichlmair A, Elliott RM, Overby AK, Glatter T, et al. (2009) NSs protein of rift valley fever virus induces the specific degradation of the double-stranded RNA-dependent protein kinase. J Virol 83: 4365–4375.
17. Filone CM, Hanna SL, Caino MC, Bambina S, Doms RW, et al. (2010) Rift valley fever virus infection of human cells and insect hosts is promoted by protein kinase C epsilon. PLoS One 5: e15483.
18. Shum D, Smith JL, Hirsch AJ, Bhinder B, Radu C, et al. (2010) High-content assay to identify inhibitors of dengue virus infection. Assay Drug Dev Technol 8: 553–570.
19. Cruz DJ, Koishi AC, Taniguchi JB, Li X, Milan Bonotto R, et al. (2013) High content screening of a kinase-focused library reveals compounds broadly-active against dengue viruses. PLoS Negl Trop Dis 7: e2073.
20. Turk B, (2006) Targeting proteases: successes, failures and future prospects. Nat Rev Drug Discov 5: 785–799.
21. Ikegami T, Narayanan K, Won S, Kamitani W, Peters CJ, et al. (2009) Rift Valley fever virus NSs protein promotes post-transcriptional downregulation of protein kinase PKR and inhibits eIF2alpha phosphorylation. PLoS Pathog 5: e1000287.
22. Morrill JC, Ikegami T, Yoshikawa-Iwata N, Lokugamage N, Won S, et al. (2010) Rapid accumulation of virulent rift valley Fever virus in mice from an attenuated virus carrying a single nucleotide substitution in the m RNA. PLoS One 5: e9986.
23. Lang SA, Kozyukov AV, Balakin KV, Skorenko AV, Ivashchenko AA, et al. (2002) Classification scheme for the design of serine protease targeted compound libraries. J Comput Aided Mol Des 16: 803–807.
24. Drosten C, Gottig S, Schilling S, Asper M, Panning M, et al. (2002) Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol 40: 2323–2330.
25. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 25: 402–408.
26. Ikegami T, Won S, Peters CJ, Makino S, (2006) Rescue of infectious rift valley fever virus entirely from cDNA, analysis of virus lacking the NSs gene, and expression of a foreign gene. J Virol 80: 2933–2940.
27. Zhang JH, Chung TD, Oldenburg KR, (1999) A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 4: 67–73.
28. Mudhasani R, Tran JP, Retterer C, Radoshitzky SR, Kota KP, et al. (2013) IFITM-2 and IFITM-3 but not IFITM-1 restrict Rift Valley fever virus. J Virol 87: 8451–8464.
29. Garcia S, Crance JM, Billecocq A, Peinnequin A, Jouan A, et al. (2001) Quantitative real-time PCR detection of Rift Valley fever virus and its application to evaluation of antiviral compounds. J Clin Microbiol 39: 4456–4461.
30. Hepojoki J, Strandin T, Wang H, Vapalahti O, Vaheri A, et al. (2010) Cytoplasmic tails of hantavirus glycoproteins interact with the nucleocapsid protein. J Gen Virol 91: 2341–2350.
31. Rogalski AA, Singer SJ, (1984) Associations of elements of the Golgi apparatus with microtubules. J Cell Biol 99: 1092–1100.
32. Doms RW, Russ G, Yewdell JW, (1989) Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum. J Cell Biol 109: 61–72.
33. Strandin T, Hepojoki J, Vaheri A, (2013) Cytoplasmic tails of bunyavirus Gn glycoproteins-Could they act as matrix protein surrogates? Virology 437: 73–80.
34. De Brabander MJ, Van de Veire RM, Aerts FE, Borgers M, Janssen PA, (1976) The effects of methyl (5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl) carbamate, (R 17934; NSC 238159), a new synthetic antitumoral drug interfering with microtubules, on mammalian cells cultured in vitro. Cancer Res 36: 905–916.
35. Jordan MA, Thrower D, Wilson L, (1992) Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis. J Cell Sci 102(Pt 3): 401–416.
36. Hendzel MJ, Wei Y, Mancini MA, Van Hooser A, Ranalli T, et al. (1997) Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106: 348–360.
37. Radtke K, Dohner K, Sodeik B, (2006) Viral interactions with the cytoskeleton: a hitchhiker's guide to the cell. Cell Microbiol 8: 387–400.