Pulmonary antigen presenting cells: Isolation, purification, and culture

Methods in Molecular Biology

Volumn 1032, Issue , 2013, Pages 19-29

  Nakano, Hideki ^a   Cook, Donald N ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

Antigen presenting cells; Autofluorescence; Culture; Dendritic cells; Flow cytometry; Gradient centrifugation; Lung; Lymph nodes; Macrophages; Monocytes; Sorting

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ANTIGEN PRESENTING CELL; ARTICLE; AUTOFLUORESCENCE; CELL CULTURE; CELL FUNCTION; CELL INTERACTION; CELL ISOLATION; CELL POPULATION; CELL SELECTION; CENTRIFUGATION; COCULTURE; CULTURE TECHNIQUE; DENDRITIC CELL; FLOW CYTOMETRY; GRADIENT ULTRACENTRIFUGATION; IMMUNOCYTOCHEMISTRY; LUNG ALVEOLUS MACROPHAGE; LYMPH NODE CELL; LYMPHOCYTE DIFFERENTIATION; MACROPHAGE; MONOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; T LYMPHOCYTE; ANIMAL; CYTOLOGY; IMMUNOLOGY; LEUKOCYTE; LUNG; LYMPH NODE; PROCEDURES;

ANIMALS; ANTIGEN-PRESENTING CELLS; CELL CULTURE TECHNIQUES; FLOW CYTOMETRY; LEUKOCYTES; LUNG; LYMPH NODES; MACROPHAGES, ALVEOLAR; MICE; T-LYMPHOCYTES;

EID: 84922782750     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-496-8_2     Document Type: Article

References (14)

1. Sertl K, Takemura T, Tschachler E, Ferrans VJ, Kaliner MA, Shevach EM (1986) Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura. J Exp Med 163:436-451 (Pubitemid 16113078)
2. Lambrecht BN, Hammad H (2009) Biology of lung dendritic cells at the origin of asthma. Immunity 31:412-424
3. Sung SS, Fu SM, Rose CE Jr, Gaskin F, Ju ST, Beaty SR (2006) A major lung CD103 (alphaE)-beta7 integrin-positive epithelial dendritic cell population expressing langerin and tight junction proteins. J Immunol 176:2161-2172 (Pubitemid 43201663)
4. Galkina E, Kadl A, Sanders J, Varughese D, Sarembock IJ, Ley K (2006) Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependent. J Exp Med 203: 1273-1282 (Pubitemid 43736596)
5. Nakano H, Free ME, Whitehead GS, Maruoka S, Wilson RH, Nakano K, Cook DN (2012) Pulmonary CD103(+) dendritic cells prime Th2 responses to inhaled allergens. Mucosal Immunol 5:53-65
6. Inaba K, Witmer-Pack MD, Inaba M, Muramatsu S, Steinman RM (1988) The function of Ia + dendritic cells and Ia-dendritic cell precursors in thymocyte mitogenesis to lectin and lectin plus interleukin. J Exp Med 167: 149-162 (Pubitemid 18093699)
7. Stevens WW, Kim TS, Pujanauski LM, Hao X, Braciale TJ (2007) Detection and quantitation of eosinophils in the murine respiratory tract by fl ow cytometry. J Immunol Methods 327: 63-74 (Pubitemid 47444310)
8. Jakubzick C, Randolph GJ (2010) Methods to study pulmonary dendritic cell migration. Methods Mol Biol 595:371-382
9. Vermaelen K, Pauwels R (2004) Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by fl ow cytometry: methodology and new insights. Cytometry A 61:170-177 (Pubitemid 39362291)
10. Naik SH, Sathe P, Park HY, Metcalf D, Proietto AI, Dakic A, Carotta S, O'Keeffe M, Bahlo M, Papenfuss A, Kwak JY, Wu L, Shortman K (2007) Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat Immunol 8:1217-1226 (Pubitemid 350011760)
11. Onai N, Obata-Onai A, Schmid MA, Ohteki T, Jarrossay D, Manz MG (2007) Identifi cation of clonogenic common Flt3 + M-CSFR + plasmacytoid and conventional dendritic cell progenitors in mouse bone marrow. Nat Immunol 8:1207-1216 (Pubitemid 350011759)
12. Cheong C, Matos I, Choi JH, Dandamudi DB, Shrestha E, Longhi MP, Jeffrey KL, Anthony RM, Kluger C, Nchinda G, Koh H, Rodriguez A, Idoyaga J, Pack M, Velinzon K, Park CG, Steinman RM (2010) Microbial stimulation fully differentiates monocytes to DC-SIGN/CD209(+) dendritic cells for immune T cell areas. Cell 143:416-429
13. Nakano H, Lin KL, Yanagita M, Charbonneau C, Cook DN, Kakiuchi T, Gunn MD (2009) Blood-derived infl ammatory dendritic cells in lymph nodes stimulate acute T helper type 1 immune responses. Nat Immunol 10:394-402
14. Nakano H, Burgents JE, Nakano K, Whitehead GS, Cheong C, Bortner CD, Cook DN (2013). Migratory properties of pulmonary dendritic cells are determined by their developmental lineage. Mucosal Immunol. 2012 Nov. doi: 10.1038/mi.2012.106. [Epub ahead of print]