Monitoring gene expression: Quantitative real-time RT-PCR

Methods in Molecular Biology

Volumn 1027, Issue , 2013, Pages 19-45

  Wagner, Elke M ^a  

^a BAXTER INNOVATIONS GMBH   (Austria)

Author keywords

Endogenous control; Liver; Macrophages; qPCR; Relative quantification; RNA extraction; RT PCR; Single well reaction

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; RNA; RNA DIRECTED DNA POLYMERASE;

ADIPOSE TISSUE; ARTICLE; CELL TYPE; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION; HUMAN; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD; ANIMAL; BIOSYNTHESIS; GENE EXPRESSION PROFILING; GENETICS; ISOLATION AND PURIFICATION; MOUSE; PROCEDURES;

ANIMALS; DNA, COMPLEMENTARY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE EXPRESSION PROFILING; MICE; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA-DIRECTED DNA POLYMERASE;

EID: 84922767387     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-369-5_2     Document Type: Article

References (19)

1. Gotto A, Pownall H (2003) Manual of lipid disorders: Reducing the risk for coronary heart disease. Lippincott, Williams and Wilkins, Philadelphia, PA.
2. Alonzi T, Mancone C, Amicone L, Tripodi M (2008) Elucidation of lipoprotein particles structure by proteomic analysis. Expert Rev Proteomics 5:91-104
3. Trevaskis NL, Charman WN, Porter CJ (2008) Lipid-based delivery systems and intestinal lymphatic drug transport: A mechanistic update. Adv Drug Deliv Rev 60:702-716
4. Wasan KM, Brocks DR, Lee SD, Sachs-Barrable K, Thornton SJ (2008) Impact of lipoproteins on the biological activity and disposition of hydrophobic drugs: Implications for drug discovery. Nat Rev Drug Discov 7:84-99
5. Danik M, Champagne D, Petit-Turcotte C, Beffert U, Poirier J (1999) Brain lipoprotein metabolism and its relation to neurodegenerative disease. Crit Rev Neurobiol 13:357-407
6. Valasek MA, Repa JJ (2005) The power of realtime PCR. Adv Physiol Educ 29:151-159
7. Bustin SA (2000) Absolute quanti fication of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193
8. Nolan T, Hands RE, Bustin SA (2006) Quanti fication of mRNA using real-time RT-CR. Nat Protoc 1:1559-1582
9. Veres G, Gibbs RA, Scherer SE, Caskey CT (1987) The molecular basis of the sparse fur mouse mutation. Science 237:415-417
10. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39:75-85
11. Vaisman BL (2010) Genotyping of transgenic animals by real-time quantitative PCR with TaqMan probes. In: Freeman LA (ed.) Methods in molecular biology: Lipoproteins, 2nd edn (Walker JM, series ed.). Humana, Totowa, NJ.
12. Real-time PCR: Understanding Ct (Application Note, 2011), Applied Bio systems™ by life technologies (2009) http://www3.applied biosystems.com/cms/groups/mcb-marketing/ documents/generaldocuments/ cms-053906.pdf
13. Real-time PCR vs. traditional PCR (Tutorial), Applied Biosystems (2009) http://www3. appliedbiosystems.com/cms/groups/mcb- marketing/documents/ generaldocuments/ cms-042484.pdf
14. Essentials of real time PCR (General Document), Applied Biosystems (2009) http://www3.appliedbiosystems.com/cms/ groups/mcb-marketing/documents/ generaldocuments/ cms-039996.pdf
15. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaf fl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611-622
16. TaqMan® gene expression assay selection guide (2009) http://www.appliedbiosystems.com/ absite/us/en/home/applications-technologies/ real-time-cr/taqman-robe-basedgene-expression-analysis/taqman-geneexpression- assay-selection-guide.html
17. Using TaqMan endogenous control assays to select an endogenous control for experimental studies (Application Note), life technologies (2009) http://www3.appliedbiosystems. com/cms/groups/mcb-marketing/documents/ generaldocuments/cms-042279.pdf
18. Applied biosystems real-time PCR systems (Reagent Guide, 2010), Applied Biosystems (2008) http://www3.appliedbiosystems. com/cms/groups/mcb-support/ documents/ generaldocuments/cms-052263.pdf
19. Freeman LA (2010) Northern analysis. In: Freeman LA (ed.) Methods in molecular biology: Lipoproteins, 2nd edn (Walker JM, series ed.). Humana, Totowa, NJ