A mix-and-measure assay for determining the activation status of endogenous Cdc42 in cytokine-stimulated macrophage cell lysates

Methods in Molecular Biology

Volumn 1172, Issue , 2014, Pages 173-184

  Miskolci, Veronika ^a   Spiering, Désirée ^a   Cox, Dianne ^a   Hodgson, Louis ^a  

^a NONE

Author keywords

Biosensor; Cdc42 activity; Fluorometry; MeroCBD; Mix and measure

Indexed keywords

MEROCYANINE; PROTEIN CDC42; REAGENT; CDC42 PROTEIN, MOUSE; CELL EXTRACT; CX3CL1 PROTEIN, MOUSE; ENHANCED GREEN FLUORESCENT PROTEIN; FRACTALKINE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; BACTERIOLYSIS; BINDING AFFINITY; CELL ASSAY; CELL LYSATE; CELL MIGRATION; CELL STIMULATION; CHEMOTAXIS; ENZYME ACTIVATION; ENZYMIC BIOSENSOR; FLUORESCENCE ANALYSIS; FLUOROMETRY; HYDROPHOBICITY; IN VITRO STUDY; MACROPHAGE; MIX AND MEASURE ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WHOLE CELL; ANIMAL; BIOASSAY; CELL LINE; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; MACROPHAGE ACTIVATION; METABOLISM; MOUSE; PROCEDURES; STAINING;

ANIMALS; BIOLOGICAL ASSAY; CDC42 GTP-BINDING PROTEIN; CELL EXTRACTS; CELL LINE; CHEMOKINE CX3CL1; ESCHERICHIA COLI; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; MACROPHAGE ACTIVATION; MACROPHAGES; MICE; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING;

EID: 84922480738     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0928-5_15     Document Type: Article

References (5)

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