Identifying essential Streptococcus sanguinis genes using genome-wide deletion mutation

Methods in Molecular Biology

Volumn 1279, Issue , 2015, Pages 15-23

  Chen, Lei ^a   Ge, Xiuchun ^a   Xu, Ping ^a  

^a NONE

Author keywords

Deletion mutation; Essential genes; Fusion PCR; Genome wide; Streptococcus sanguinis

Indexed keywords

AMPLICON; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; DNA FLANKING REGION; ESSENTIAL GENE; GENE CASSETTE; GENE DELETION; GENE MUTATION; MUTANT; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS SANGUINIS; GENETIC TRANSFORMATION; GENETICS; METABOLISM;

STREPTOCOCCUS SANGUINIS;

PRIMER DNA;

DNA PRIMERS; GENES, BACTERIAL; GENES, ESSENTIAL; SEQUENCE DELETION; STREPTOCOCCUS SANGUIS; TRANSFORMATION, GENETIC;

EID: 84922460388     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2398-4_2     Document Type: Article

References (12)

1. Juhas M, Eberl L, Church GM (2012) Essential genes as antimicrobial targets and cornerstones of synthetic biology. Trends Biotechnol 30:601–607
2. Xu P, Ge X, Chen L, Wang X, Dou Y et al (2011) Genome-wide essential gene identifi - cation in Streptococcus sanguinis. Sci Rep 1:125
3. Chen L, Ge X, Wang X, Patel JR, Xu P (2012) SpxA1 involved in hydrogen peroxide production, stress tolerance and endocarditis virulence in Streptococcus sanguinis. PLoS One 7(6):e40034
4. Nyvad B, Kilian M (1987) Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand J Dent Res 95:369–380
5. Mylonakis E, Calderwood SB (2001) Infective endocarditis in adults. N Engl J Med 345:1318–1330
6. Paik S, Senty L, Das S, Noe JC, Munro CL et al (2005) Identifi cation of virulence determinants for endocarditis in Streptococcus sanguinis by signature-tagged mutagenesis. Infect Immun 73:6064–6074
7. Xu P, Alves JM, Kitten T, Brown A, Chen Z et al (2007) Genome of the opportunistic pathogen Streptococcus sanguinis. J Bacteriol 189:3166–3175
8. Ge X, Xu P (2012) Genome-wide gene deletions in Streptococcus sanguinis by high throughput PCR. J Vis Exp 69:e4356
9. Gaustad P, Havardstein LS (1997) Competence-pheromone in Streptococcus sanguis. Identifi cation of the competence gene comC and the competence pheromone. Adv Exp Med Biol 418:1019–1021
10. Das S, Kanamoto T, Ge X, Xu P, Unoki T et al (2009) Contribution of lipoproteins and lipoprotein processing to endocarditis virulence in Streptococcus sanguinis. J Bacteriol 191: 4166–4179
11. Turner LS, Das S, Kanamoto T, Munro CL, Kitten T (2009) Development of genetic tools for in vivo virulence analysis of Streptococcus sanguinis. Microbiology 155:2573–2582
12. De Berardinis V, Vallenet D, Castelli V, Besnard M, Pinet A et al (2008) A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol 4:174