MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay

Journal of Microbiological Methods

Volumn 111, Issue , 2015, Pages 21-23

  Chong, Patrick M ^a   McCorrister, Stuart J ^a   Unger, Mark S ^a   Boyd, David A ^a   Mulvey, Michael R ^a   Westmacott, Garrett R ^a  

^a PUBLIC HEALTH AGENCY OF CANADA   (Canada)

Author keywords

Acinetobacter; Carbapenemase detection; Enterobacteriaceae; MALDI TOF; Pseudomonas

Indexed keywords

CARBAPENEMASE; BACTERIAL PROTEIN; BETA LACTAMASE;

ACINETOBACTER BAUMANNII; ARTICLE; BACTERIUM ISOLATE; CARBA NP PHENOTYPIC ASSAY; CITROBACTER FREUNDII; CONTROLLED STUDY; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; INTERMETHOD COMPARISON; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEASUREMENT ACCURACY; MORGANELLA MORGANII; NONHUMAN; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; COMPARATIVE STUDY; ENTEROBACTERIACEAE; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; MICROBIOLOGICAL EXAMINATION; PROCEDURES;

ACINETOBACTER; ACINETOBACTER BAUMANNII; ENTEROBACTERIACEAE; ENTEROBACTERIACEAE SPP.; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

ACINETOBACTER BAUMANNII; BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; BETA-LACTAMASES; ENTEROBACTERIACEAE; MASS SPECTROMETRY; PSEUDOMONAS AERUGINOSA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84922345709     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2015.01.024     Document Type: Article

References (20)

1. Braykov N.P., Eber M.R., Klein E.Y., Morgan D.J., Laxminarayan R. Trends in resistance to carbapenems and third-generation cephalosporins among clinical isolates of Klebsiella pneumoniae in the United States, 1999-2010. Infect. Control Hosp. Epidemiol. 2013, 34:259-268.
2. Dortet L., Poirel L., Nordmann P. Rapid detection of carbapenemase-producing Pseudomonas spp. J. Clin. Microbiol. 2012, 50:3773-3776.
3. Hoyos-Mallecot Y., Cabrera-Alvargonzalez J.J., Miranda-Casas C., Rojo-Martin M.D., Liebana-Martos C., Navarro-Mari J.M. MALDI-TOF MS, a useful instrument for differentiating metallo-beta-lactamases in Enterobacteriaceae and Pseudomonas spp. Lett. Appl. Microbiol. 2014, 58:325-329.
4. Hrabak J. Detection of carbapenemases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) meropenem hydrolysis assay. Methods Mol. Biol. 2015, 1237:91-96.
5. Hrabak J., Walkova R., Studentova V., Chudackova E., Bergerova T. Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 2011, 49:3222-3227.
6. Hrabak J., Studentova V., Walkova R., Zemlickova H., Jakubu V., Chudackova E., et al. Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 2012, 50:2441-2443.
7. Johansson A., Ekelof J., Giske C.G., Sundqvist M. The detection and verification of carbapenemases using ertapenem and matrix assisted laser desorption ionization-time of flight. BMC Microbiol. 2014, 14. (pp. 89-2180-14-89).
8. Kempf M., Bakour S., Flaudrops C., Berrazeg M., Brunel J.M., Drissi M., et al. Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time of flight mass spectrometry. PLoS One 2012, 7:e31676.
9. Knox J., Jadhav S., Sevior D., Agyekum A., Whipp M., Waring L., et al. Phenotypic detection of carbapenemase-producing Enterobacteriaceae by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Carba NP test. J. Clin. Microbiol. 2014, 52:4075-4077.
10. Miriagou V., Cornaglia G., Edelstein M., Galani I., Giske C.G., Gniadkowski M., et al. Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues. Clin. Microbiol. Infect. 2010, 16:112-122.
11. Nagy E., Abrok M., Bartha N., Bereczki L., Juhasz E., Kardos G., et al. Special application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiological diagnostics. Orv. Hetil. 2014, 155:1495-1503.
12. Nordmann P., Naas T., Poirel L. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg. Infect. Dis. 2011, 17:1791-1798.
13. Nordmann P., Dortet L., Poirel L. Carbapenem resistance in Enterobacteriaceae: here is the storm!. Trends Mol. Med. 2012, 18:263-272.
14. Nordmann P., Poirel L., Dortet L. Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg. Infect. Dis. 2012, 18:1503-1507.
15. Papp-Wallace K.M., Endimiani A., Taracila M.A., Bonomo R.A. Carbapenems: past, present, and future. Antimicrob. Agents Chemother. 2011, 55:4943-4960.
16. Queenan A.M., Bush K. Carbapenemases: the versatile beta-lactamases. Clin. Microbiol. Rev. 2007, 20:440-458. (table of contents).
17. Sauget M., Cabrolier N., Manzoni M., Bertrand X., Hocquet D. Rapid, sensitive and specific detection of OXA-48-like-producing Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Microbiol. Methods 2014, 105:88-91.
18. Schwaber M.J., Klarfeld-Lidji S., Navon-Venezia S., Schwartz D., Leavitt A., Carmeli Y. Predictors of carbapenem-resistant Klebsiella pneumoniae acquisition among hospitalized adults and effect of acquisition on mortality. Antimicrob. Agents Chemother. 2008, 52:1028-1033.
19. Tijet N., Boyd D., Patel S.N., Mulvey M.R., Melano R.G. Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 2013, 57:4578-4580.
20. Wang L.J., Fan Y.Y., Wang M., Lu X.X. Detection of carbapenem-producing Enterobacteriaceae by MALDI-TOF MS. Zhonghua Yi Xue Za Zhi 2013, 93:2079-2081.