Analysis of in vivo transcription factor recruitment by chromatin immunoprecipitation of mouse embryonic kidney

Methods in Molecular Biology

Volumn 886, Issue , 2012, Pages 275-291

  Heliot, Claire ^a   Cereghini, Silvia ^a  

^a INSERM   (France)

Author keywords

ChIP protocol; Chromatin immunoprecipitation; Metanephric kidneys; Mouse; Transcription factor

Indexed keywords

DNA; FORMALDEHYDE; PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BINDING SITE; CELL CULTURE; CELL LINE; CHROMATIN; CHROMATIN IMMUNOPRECIPITATION; DNA CROSS LINKING; DNA PROTEIN COMPLEX; DNA SEQUENCE; EMBRYO; EMBRYO DEVELOPMENT; GENE CONTROL; GENETIC ASSOCIATION; IN VIVO STUDY; KIDNEY; KIDNEY DEVELOPMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; REAL TIME POLYMERASE CHAIN REACTION; ULTRASOUND; ANIMAL; CHEMISTRY; DISSECTION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; POLYMERASE CHAIN REACTION; PRENATAL DEVELOPMENT;

ANIMALS; CHROMATIN; CHROMATIN IMMUNOPRECIPITATION; DISSECTION; DNA; KIDNEY; MICE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SONICATION; TRANSCRIPTION FACTORS;

EID: 84922058120     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-851-1_25     Document Type: Article

References (13)

1. Cao Y, Yao Z, Sarkar D et al (2010) Genomewide MyoD binding in skeletal muscle cells: a potential for broad cellular reprogramming. Dev Cell 18:662-674
2. Xu J, Watts JA, Pope SD et al (2009) Transcriptional competence and the active marking of tissue-speci fi c enhancers by de fi ned transcription factors in embryonic and induced pluripotent stem cells. Genes Dev 23: 2824-2838
3. Collas P, Dahl JA (2008) Chop it, ChIP it, check it: the current status of chromatin immunoprecipitation. Front Biosci 13:929-943
4. Boyd KE, Farnham PJ (1999) Coexamination of site-speci fi c transcription factor binding and promoter activity in living cells. Mol Cell Biol 19:8393-8399
5. Orlando V, Strutt H, Paro R (1997) Analysis of chromatin structure by in vivo formaldehyde cross-linking. Methods (San Diego, CA) 11: 205-214
6. Lagha M, Kormish JD, Rocancourt D et al (2008) Pax3 regulation of FGF signaling affects the progression of embryonic progenitor cells into the myogenic program. Genes Dev 22: 1828-1837
7. Parrizas M, Maestro MA, Boj SF et al (2001) Hepatic nuclear factor 1-alpha directs nucleosomal hyperacetylation to its tissue-speci fi c transcriptional targets. Mol Cell Biol 21: 3234-3243
8. Havis E, Anselme I, Schneider-Maunoury S (2006) Whole embryo chromatin immunoprecipitation protocol for the in vivo study of zebra fi sh development. BioTechniques 40:34, 36, 38 passim
9. Lokmane L, Heliot C, Garcia-Villalba P, Fabre M, Cereghini S (2010) vHNF1 functions in distinct regulatory circuits to control ureteric bud branching and early nephrogenesis. Development (Cambridge, UK) 137:347-357
10. Stock JK, Giadrossi S, Casanova M et al (2007) Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells. Nat Cell Biol 9: 1428-1435
11. Haring M, Offermann S, Danker T et al (2007) Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization. Plant Methods 3:11
12. Verdeguer F, Le Corre S, Fischer E et al (2010) A mitotic transcriptional switch in polycystic kidney disease. Nat Med 16:106-110
13. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods (San Diego, CA) 25: 402-408