The fused anthranilate synthase from Streptomyces venezuelae functions as a monomer

Molecular and Cellular Biochemistry

Volumn 400, Issue 1-2, 2015, Pages 9-15

  Ashenafi, Meseret ^a   Reddy, Prasad T ^b,c   Parsons, James F ^c   Byrnes, W Malcolm ^a  

^a HOWARD UNIVERSITY COLLEGE OF MEDICINE   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

Anthranilate synthase; Chorismate utilizing enzyme; Fused enzyme; Streptomyces venezuelae

Indexed keywords

AMMONIA; ANTHRANILATE SYNTHASE; CHORISMIC ACID; GLUTAMINE; MONOMER; TRYPTOPHAN;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; ENZYME ACTIVITY; ENZYME INHIBITION; GEL FILTRATION CHROMATOGRAPHY; LIGHT SCATTERING; MICHAELIS CONSTANT; MOLECULAR WEIGHT; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SECONDARY METABOLISM; STEADY STATE; STREPTOMYCES VENEZUELAE; CATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; PROTEIN TERTIARY STRUCTURE; STREPTOMYCES;

AMINO ACID SEQUENCE; ANTHRANILATE SYNTHASE; CATALYSIS; KINETICS; PROTEIN STRUCTURE, TERTIARY; STREPTOMYCES; SUBSTRATE SPECIFICITY; TRYPTOPHAN;

EID: 84922004627     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-014-2256-3     Document Type: Article

References (28)

1. Kane JF, Jensen RA (1970) The molecular aggregation of anthranilate synthase in Bacillus subtilis. Biochem Biophys Res Commun 41:328–333
2. Patel N, Holmes WM, Kane JF (1974) Homologous and hybrid complexes of anthranilate synthase from Bacillus species. J Bacteriol 119:220–227
3. Sawula RV, Crawford IP (1973) Anthranilate synthetase of Acinetobactercalcoaceticus: separation and partial characterization of subunits. J Biol Chem 248:3573–3581
4. Queener SW, Queener SF, Meeks JR, Gunsalus IC (1973) Anthranilate synthase from Pseudomonas putida: purification and properties of a two-component enzyme. J Biol Chem 248:151–161
5. Patel N, Holmes WM, Kane JF (1973) Intergeneric complementation of anthranilate synthase subunits. J Bacteriol 114:600–602
6. Kane JF, Holmes WM, Smiley KL, Jensen RA (1973) Rapid regulation of an anthranilate synthase aggregate by hysteresis. J Bacteriol 113:224–232
7. De Troch P, Dosselaere F, Keijers V, de Wilde P, Vanderleyden J (1997) Isolation and characterization of the Azospirillum brasilense trpE(G) gene, encoding anthranilate synthase. Curr Microbiol 34:27–32
8. Bae YM, Holmgren E, Crawford IP (1989) Rhizobium meliloti anthranilate synthase gene: cloning, sequence, and expression in Escherichia coli. J Bacteriol 171:3471–3478
9. Paradkar AS, Stuttard C, Vining LC (1991) Molecular cloning of the genes for anthranilate synthetase from Streptomyces venezuelae ISP 5230. FEMS Microbiol Lett 62:177–181
10. Baker TI, Crawford IP (1969) Anthranilate synthase: partial purification and some kinetic studies on the enzyme from Escherichia coli. J Biol Chem 241:5577–5584
11. Tamir H, Srinivasan PR (1969) Purification and properties of anthranilate synthase from Salmonella typhimurium. J Biol Chem 244:6507–6513
12. Henderson EJ, Nagano H, Zalkin H, Hwang LH (1970) The anthranilate synthetase-anthranilate 5-phosphoribosyltransferase complex of Salmonellatyphimurium. Implications concerning the mode of assembly of the complex. Biochemistry 13:1416–1423
13. Bauerle R, Hess J, French S (1987) Anthranilate synthase-anthranilate phosphoribosyltransferase complex and subunits of Salmonella typhimurium. Methods Enzymol 142:366–386
14. Zalkin H, Hwang LH (1971) Anthranilate synthetase from Serratia marcescens: on the properties and relationship to the enzyme from Salmonella typhimurium. J Biol Chem 246:6899–6907
15. Robb F, Hutchinson MA, Belser WL (1971) Anthranilate synthetase: some physical and kinetic properties of the enzyme from Serratia marcescens. J Biol Chem 246:6908–6912
16. Tutino ML, Tosco A, Marino G, Sannia G (1997) Expression of Sulfolobussolfataricus trpE and trpG genes in E. coli. Biochem Biophys Res Commun 230:306–310
17. Byrnes WM, Vilker VL (2004) Extrinsic factors potassium chloride and glycerol induce thermostability in recombinant anthranilate synthase from Archaeoglobusfulgidus. Extremophiles 8:455–462
18. Morollo AA, Eck MJ (2001) Structure of the cooperative allosteric anthranilate synthase from Salmonella typhimurium. Nature Struct Biol 8:243–247
19. Spraggon G, Kim C, Nguyen-Huu X, Yee M-C, Yanofsky C, Mills SE (2001) The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate and glutamine, and a product, glutamate, and (ii) its end-product inhibitor, l-tryptophan. Proc Natl Acad Sci USA 98:6021–6026
20. Knöchel T, Ivens A, Hester G, Gonzalez A, Bauerle R, Wilmanns M, Kirschner K, Jansonius JN (1999) The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications. Proc Natl Acad Sci USA 96:9479–9484
21. Zalkin H, Kling D (1968) Anthranilate synthetase. Purification and properties of component I from Salmonella typhimurium. Biochemistry 7:3566–3573
22. Ashenafi M, Carrington R, Collins AC, Byrnes WM (2008) The fused TrpEG from Streptomyces venezuelae is an anthranilate synthase, not a 2-amino-2-deoxyisochorismate (ADIC) synthase. Ethn Dis 18(S2):9–13
23. Lin C, Paradkar AS, Vining LC (1998) Regulation of an anthranilate synthase gene in Streptomyces venezuelae by a trp attenuator. Microbiol 144:1971–1980
24. Grisostomi C, Kast P, Pulido R, Huynh J, Hilvert D (1997) Efficient in vivo synthesis and rapid purification of chorismic acid using an engineered Escherichia coli strain. Bioorg Chem 25:297–305
25. Addadi L, Jaffe EK, Knowles JR (1983) Secondary tritium isotope effects as probes of the enzymic and nonenzymic conversion of chorismate to prephenate. Biochemistry 22:4494–4501
26. Caligiuri MG, Bauerle R (1991) Subunit communication in the anthranilate synthase complex from Salmonella typhimurium. Science 252:1845–1848
27. Li QA, Mavrodi DV, Thomashow LS, Roessle M, Blankenfeldt W (2011) Ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (ADIC) synthase PhzE. J Biol Chem 286:18213–18221
28. Ziebart KT, Dixon SM, Avila B, El-Badri MH, Guggenheim KG, Kurth MJ, Toney MD (2010) Targeting multiple chorismate-utilizing enzymes with a single inhibitor: validation of a three-stage design. J Med Chem 53:3718–3729