Simultaneous characterization of instantaneous young’s modulus and specific membrane capacitance of single cells using a microfluidic system

Sensors (Switzerland)

Volumn 15, Issue 2, 2015, Pages 2763-2773

  Zhao, Yang ^a   Chen, Deyong ^a   Luo, Yana ^a   Chen, Feng ^a   Zhao, Xiaoting ^b   Jiang, Mei ^b   Yue, Wentao ^b   Long, Rong ^c   Wang, Junbo ^a   Chen, Jian ^a  

^a INSTITUTE OF ELECTRONICS   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Cellular biophysics; Instantaneous Young s modulus; Microfluidics; Single cell analysis; Specific membrane capacitance

Indexed keywords

CAPACITANCE; CELL CULTURE; CELLS; HIGH SPEED CAMERAS; MEMBRANES; MICROFLUIDICS;

BIOPHYSICAL PROPERTIES; CELLULAR BIOPHYSICS; DISTRIBUTION CIRCUITS; IMPEDANCE PROFILES; MEMBRANE CAPACITANCES; MICRO FLUIDIC SYSTEM; NON SMALL CELL LUNG CANCER; SINGLECELL ANALYSIS;

CYTOLOGY;

CELL MEMBRANE; CHEMISTRY; GENETIC PROCEDURES; HUMAN; IMPEDANCE; MICROFLUIDIC ANALYSIS; SINGLE CELL ANALYSIS;

BIOSENSING TECHNIQUES; CELL MEMBRANE; ELECTRIC IMPEDANCE; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; SINGLE-CELL ANALYSIS;

EID: 84921899495     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s150202763     Document Type: Article

References (21)

1. Ethier, C.R.; Simmons, C.A. Introductory Biomechanics: From Cells to Organisms; Cambridge University Press: Cambridge, UK, 2007.
2. Lee, G.Y.H.; Lim, C.T. Biomechanics approaches to studying human diseases. Trends Biotechnol. 2007, 25, 111-118.
3. Suresh, S. Biomechanics and biophysics of cancer cells. Acta Biomater. 2007, 3, 413-438.
4. Kim, D.H.; Wong, P.K.; Park, J.; Levchenko, A.; Sun, Y. Microengineered platforms for cell mechanobiology. Ann. Rev. Biomed. Eng. 2009, 11, 203-233.
5. Valero, A.; Braschler, T.; Renaud, P. A unified approach to dielectric single cell analysis: Impedance and dielectrophoretic force spectroscopy. Lab Chip 2010, 10, 2216-2225.
6. Becker, F.F.; Wang, X.B.; Huang, Y.; Pethig, R.; Vykoukal, J.; Gascoyne, P.R.C. Separation of human breast-cancer cells from blood by differential dielectric affinity. Proc. Natl. Acad. Sci. USA 1995, 92, 860-864.
7. Cross, S.E.; Jin, Y.S.; Rao, J.; Gimzewski, J.K. Nanomechanical analysis of cells from cancer patients. Nat. Nanotechnol. 2007, 2, 780-783.
8. Mao, X.; Huang, T.J. Exploiting mechanical biomarkers in microfluidics. Lab Chip 2012, 12, 4006-4009.
9. Zheng, Y.; Sun, Y. Microfluidic devices for mechanical characterisation of single cells in suspension. Micro Nano Lett. 2011, 6, 327-331.
10. Cheung, K.C.; di Berardino, M.; Schade-Kampmann, G.; Hebeisen, M.; Pierzchalski, A.; Bocsi, J.; Mittag, A.; Tárnok, A. Microfluidic impedance-based flow cytometry. Cytometry A. 2010, 77, 648-666.
11. Yobas, L. Microsystems for cell-based electrophysiology. J. Micromech. Microeng. 2013, 23, 83002.
12. Luo, Y.; Chen, D.; Zhao, Y.; Wei, C.; Zhao, X.; Yue, W.; Long, R.; Wang, J.B.; Chen, J. A constriction channel based microfluidic system enabling continuous characterization of cellular instantaneous Young’s modulus. Sens. Actuators B Chem. 2014, 202, 1183-1189.
13. Zhao, Y.; Chen, D.; Luo, Y.; Li, H.; Deng, B.; Huang, S.-B.; Chiu, T.-K.; Wu, M.-H.; Long, R.; Hu, H., et al. A microfluidic system for cell type classification based on cellular size-independent electrical properties. Lab Chip 2013, 13, 2272-2277.
14. Zhao, Y.; Zhao, X.T.; Chen, D.Y.; Luo, Y.N.; Jiang, M.; Wei, C.; Long, R.; Yue, W.T.; Wang, J.B.; Chen, J. Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity. Biosens. Bioelectron. 2014, 57, 245-253.
15. Cho, Y.H.; Yamamoto, T.; Sakai, Y.; Fujii, T.; Kim, B. Development of microfluidic device for electrical/physical characterization of single cell. J. Microelectromech. Syst. 2006, 15, 287-295.
16. Chen, J.; Zheng, Y.; Tan, Q.; Zhang, Y.L.; Li, J.; Geddie, W.R.; Jewett, M.A.S.; Sun, Y. A microfluidic device for simultaneous electrical and mechanical measurements on single cells. Biomicrofluidics 2011, 5, 14113.
17. Chen, J.; Zheng, Y.; Tan, Q.; Shojaei-Baghini, E.; Zhang, Y.L.; Li, J.; Prasad, P.; You, L.; Wu, X.Y.; Sun, Y. Classification of cell types using a microfluidic device for mechanical and electrical measurement on single cells. Lab Chip 2011, 11, 3174-3181.
18. Zheng, Y.; Shojaei-Baghini, E.; Azad, A.; Wang, C.; Sun, Y. High-throughput biophysical measurement of human red blood cells. Lab Chip 2012, 12, 2560-2567.
19. Zheng, Y.; Nguyen, J.; Wei, Y.; Sun, Y. Recent advances in microfluidic techniques for single-cell biophysical characterization. Lab Chip 2013, 13, 2464-2483.
20. Zhao, Y.; Chen, D.; Li, H.; Luo, Y.; Deng, B.; Huang, S.B.; Chiu, T.-K.; Wu, M.-H.; Long, R.; Hu, H.; et al. A microfluidic system enabling continuous characterization of specific membrane capacitance and cytoplasm conductivity of single cells in suspension. Biosens. Bioelectron. 2013, 43C, 304-307.
21. Huang, S.B.; Zhao, Z.; Chen, D.Y.; Lee, H.C.; Luo, Y.N.; Chiu, T.K.; Wang, J.; Chen, J.; Wu, M.-H. A clogging-free microfluidic platform with an incorporated pneumatically-driven membrane-based active valve enabling specific membrane capacitance and cytoplasm conductivity characterization of single cells. Sens. Actuators B Chem. 2014, 190, 928-936.