Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate

Apoptosis and Cancer: Methods and Protocols: Second Edition

Volumn , Issue , 2014, Pages 11-20

  Diaz, David ^a   Prieto, Alfredo ^a   Reyes, Eduardo ^a   Barcenilla, Hugo ^a   Monserrat, Jorge ^a   Alvarez Mon, Melchor ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Accurate apoptosis measurement; Annexin V; Antigen loss; Apoptosis; Apoptotic index; Apoptotic rate; Cell enumeration; Cell fragmentation; Microbeads

Indexed keywords

EID: 84921790660     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-1-4939-1661-0_2     Document Type: Chapter

References (25)

1. Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555-556
2. Russell JH, Dobos CB (1980) Mechanisms of immune lysis. II. CTL-induced nuclear disintegration of the target begins within minutes of cell contact. J Immunol 125:1256-1261
3. Cohen JJ, Duke RC (1984) Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. J Immunol 132:38-42
4. Khong HT, Restifo NP (2002) Natural selection of tumor variants in the generation of "tumor escape" phenotypes. Nat Immunol 3:999-1005
5. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F (1997) Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 27:1-20
6. Herault O, Colombat P, Domenech J, Degenne M, Bremond JL, Sensebe L, Bernard MC, Binet C (1999) A rapid single-laser flow cytometric method for discrimination of early apoptotic cells in a heterogeneous cell population. Br J Haematol 104:530-537
7. van Engeland M, Nieland LJW, Ramaekers FCS, Schutte B, Reutelingsperger CPM (1998) Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31:1-9
8. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger CPM (1995) A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184:39-51
9. Gorczyca W, Gong J, Darzynkiewicz Z (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 52:1945-1951
10. Gong J, Traganos F, Darzynkiewicz Z (1994) A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal Biochem 218:314-319
11. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV (1992) Dead cell discrimination with 7-aminoactinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13:204-208
12. Pantaleo G, Graziosi C, Demarest JF, Butini L, Montroni M, Fox CH, Orenstein JM, Kotler DP, Fauci AS (1993) HIV infection is active and progressive in lymphoid tissue during the clinically latent stage of disease. Nature 362:355-358
13. Ashkenazi A, Dixit VM (1998) Death receptors:signaling and modulation. Science 281:1305-1308
14. Koopman G, Reutelingsperger CPM, Kuijten GA, Keehnen RM, Pals ST, van Oers MH (1994) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415-1420
15. Devaux PF (1991) Static and dynamic lipid asymmetry in cell membranes. Biochemistry 30:1163-1173
16. Zachowski A (1993) Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement. Biochem J 294:1-14
17. Fadok VA, Voelker DR, Campbell PA, Bratton DL, Cohen JJ, Noble PW, Riches DW, Henson PM (1993) The ability to recognize phosphatidylserine on apoptotic cells is an inducible function in murine bone marrow-derived macrophages. Chest 103:102
18. Potten CS (1996) What is an apoptotic index measuring? A commentary. Br J Cancer 74:1743-1748
19. Darzynkiewicz Z, Traganos F (1998) Measurement of apoptosis. Adv Biochem Eng Biotechnol 62:33-73
20. Darzynkiewicz Z, Bedner E, Traganos F, Murakami T (1998) Critical aspects in the analysis of apoptosis and necrosis. Hum Cell 11:3-12
21. Prieto A, Díaz D, Barcenilla H, García Suárez J, Reyes E, Monserrat J, San Antonio E, Melero D, de la Hera A, Orfao A, Álvarez Mon-Soto M (2002) Apoptotic rate: a new indicator for the quantification of the occurrence of apoptosis in cell culture. Cytometry 48:185-193
22. Potter A, Kim C, Golladon KA, Rabinovith PS (1999) Apoptotic human lymphocytes have diminished CD4 and CD8 receptor expression. Cell Immunol 193:36-47
23. Philippé J, Louagie H, Thierens H, Vral A, Cornelissen M, De Ridder L (1997) Quantification of apoptosis in lymphocyte subsets and effect of apoptosis on apparent expression of membrane antigens. Cytometry 29:242-249
24. Diaz D, Prieto A, Barcenilla H, Monserrat J, Prieto P, Sanchez MA, Reyes E, Hernandez-Fuentes MP, de la Hera A, Orfao A, Alvarez-Mon M (2004) Loss of lineage antigens is a common feature of apoptotic lymphocytes. J Leukoc Biol 76:609-615
25. Prieto A, Reyes E, Diaz D, Hernandez-Fuentes MP, Monserrat J, Perucha E, Munoz L, Vangioni R, de la Hera A, Orfao A, Alvarez-Mon M (2000) A new method for the simultaneous analysis of growth and death of immunophenotypically defined cells in culture. Cytometry 39:56-66