A rapid single-laser flow cytometric method for discrimination of early apoptotic cells in a heterogenous cell population

British Journal of Haematology

Volumn 104, Issue 3, 1999, Pages 530-537

  Herault, Olivier ^a,b   Colombat, Philippe ^a,b   Domenech, Jorge ^a,b   Degenne, Michel ^b   Bremond, Jean Louis ^b   Sensebe, Luc ^c   Bernard, Marie Christine ^a   Binet, Christian ^a,b  

^a UNIVERSITÉ DE TOURS   (France)

^b UNIVERSITY HOSPITAL OF TOURS   (France)

^c Regional Blood Bank   (France)

Author keywords

7 amino actinomycin D; Annexin V; Apoptosis; Flow cytometry; Heterogenous cell population

Indexed keywords

APOPTOSIS; ARTICLE; CELL DEATH; CELL SELECTION; CELL STRAIN HL 60; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LASER; PRIORITY JOURNAL;

EID: 0033057045     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.1999.01203.x     Document Type: Article

References (33)

1. Andree, H.A., Reutelingsperger, C.P., Hautpmann, R., Hemker, H.C., Hermens, W.T. & Willems, G.M. (1990) Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers. Journal of Biological Chemistry, 265, 4923-4928.
2. Arends, M.J., Morris, R.G. & Wyllie, A.H. (1990) Apoptosis: the role of endonucleases. American Journal of Pathology, 136, 593-608.
3. Blair, O.C., Carbone, R. & Sartorelli, A.C. (1985) Differentiation of HL-60 promyelocytic leukemia cells monitored by flow cytometric measurement of nitro blue tetrazolium (NBT) reduction. Cytometry. 6, 54-61.
4. Carbonari, M., Cibati, M., Cherchi, M., Sbarigia, D., Pesce, A.M., Dell Anna, L., Modica, A. & Fiorilli, M. (1994) Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method. Blood, 83, 1268-1277.
5. Chrest, F.J., Buchholz, M.A., Kim, Y.H., Kwon, T.K. & Nordin, A.A. (1993) Identification and quanlitation of apoptotic cells following anti-CD3 activation of murine G0 T cells. Cytometry, 14, 883-890.
6. Cowden, R.R. & Curtis, S.K. (1981) Microfluorometric investigations of chromatin structure. I. Evaluation of nine DNA-specific fluorochromes as probes of chromatin organization. Histochemistry, 72, 11-23.
7. Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Holtz, M.A., Lassota, P. & Traganos, F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry, 13, 795-808.
8. Darzynkiewicz, Z., Juan, G., Li. X., Gorczyca, W., Murakami, T. & Traganos, F. (1997) Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry, 27, 1-20.
9. Del Vecchio, M.T., Leoncini, L., Buerki, K., Kraft, R., Megha, T., Barbini, P., Tosi, P. & Cottier, H. (1991) Diffuse centrocytic and/or centroblastic malignant non-Hodgkin's lymphomas: comparison of mitotic and pycnotic (apoptotic) indices. International Journal of Cancer, 47, 38-43.
10. Dransfield, I., Buckle, A.M., Savill. J.S., McDowall, A., Haslett, C. & Hogg, N. (1994) Neutrophil apoptosis is associated with a reduction in CD16 (Fc gamma RIII) expression. Journal of Immunology, 53, 1254-1263.
11. Dransfield, I., Stocks, S.C. & Haslett, C. (1995) Regulation of cell adhesion molecule expression and function associated with neutrophil apoptosis. Blood, 85, 3264-3273.
12. Fadok, V.A., Voelker, D.R., Campbell, P.A., Cohen, J.J., Bratton, D.L., Henson, P.M. (1992) Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. Journal of Immunology, 148, 2207-2216.
13. Gill, J.E., Jotz, M.M., Young, S.G., Modest, E.J. & Sengugta, S.K. (1975) 7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties. Journal of Histochemistry and Cytochemistry, 23, 793-799.
14. Homburg, C.H., de Hass, M., von dem Borne, A.E., Verhoeven, A.J., Reutelingsperger, C.P. & Roos, D. (1995) Human neutrophils lose their surface Fc gamma RIII and acquire annexin V binding sites during apoptosis in vitro. Blood, 85, 532-540.
15. Jordan, C.T., Yamasaki, G. & Minamoto, D. (1996) High-resolution cell cycle analysis of defined phenotypic subsets within primitive human hematopoietic cell populations. Experimental Hematology, 24, 1347-1355.
16. Keller, J.R., Ortiz, M. & Ruscetti, F.W. (1995) Stell Factor (c-kit ligand) promotes the survival of hematopoietic stem/progenitor cells in the absence of cell division. Blood, 86, 1757-1764.
17. Kerr, J.F., Wyllie, A.H. & Currie, A.R. (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. British Journal of Cancer, 26, 239-257.
18. Koopman, G., Reutelingsperger, C.P., Kuijten, G.A., Keehnen, R.M., Pals, S.T. & van Oers, M.H. (1994) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis, Blood, 84, 1415-1420.
19. Lennon, S.V., Martin, S.J. & Cotter, T.G. (1991) Dose-dependent induction apoptosis in human tumour cell lines by widely diverging stimuli. Cell Proliferation, 24, 203-214.
20. Majno, G. & Joris, I. (1995) Apoptosis, oncosis, and necrosis: an overview of cell death. American Journal of Pathology, 146, 3-15.
21. Martin, S.J., Bradley, J.G. & Cotter, T.G. (1990) HL60 cells induced to differentiate towards neutrophils subsequently die via apoptosis. Clinical and Experimental Immunology, 79, 448-453.
22. Martin, S.J., Reutelingsperger, C.P.M., McGahon, A.J., Rader, J.A., van Schie, R.C.A.A., LaFace, D.M. & Green, D.R. (1995) Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. Journal of Experimental Medicine, 182, 1545-1556.
23. Palissot, V, Liautaud-Roger, F., Carpentier, Y. & Dufer, J. (1996) Image cytometry of early nuclear events during apoptosis induced by camptothecin in HL-60 leukemic cells. Cytometry, 25, 341-348.
24. Philpott, N.J., Turner, A.J.C., Scopes, J., Westby, M., Marsh, J.C.W., Gordon-Smith, E.C., Dalgleish, A.G. & Gibson, P.M. (1996) The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques. Blood, 87, 2244-2251.
25. Sarraf, C.E. & Bowen, I.D. (1988) Proportions of mitotic and apoptotic cells in a range of untreated experimental tumours. Cell and Tissue Kinetics, 21, 45-49.
26. Schmid, I., Krall, W.J., Uittenbogaart, C.H., Braun, J. & Giorgi, J.V. (1992) Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry, 13, 204-208.
27. Schmid, I., Uittenbogaart, C.H., Keld, B. & Giorgi, J.V. (1994) A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. Journal of Immunological Methods, 170, 145-157.
28. + thymocytes by flow cytometry. Journal of Immunological Methods, 137, 79-87.
29. Vermes, I., Hannen, C., Steffens-Nakken, H. & Reutelingsperger, C. (1995) A novel assay for apoptosis: flow cytometric detection or phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V. Journal of Immunological Methods, 184, 39-51.
30. Williams, G.T., Smith, C.A., Spooncer, E., Dexter, T.M. & Taylor, D.R. (1990) Haematopoietic colony stimulating factors promote cell survival by suppressing apoptosis. Nature, 343, 76-79.
31. Wyllie, A.H., Morris, R.G., Smith, A.L. & Dunlop, D. (1984) Chromatin cleavage in apoptosis: assocation with condensed chromatin morphology and dependence on macromolecular synthesis. Journal of Pathology, 142, 67-77.
32. + cells in the presence of various combinations of recombinant cytokines under serum-free culture conditions. British Journal of Haematology, 86, 461-467.
33. Zelenin, A.V., Poletaev, A.I., Stepanova, N.G., Barsky, V.E., Kolesnikov, V.A., Nikitin, S.G., Zhule, A.I. & Gnutchev, N.V. (1984) 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry, 5, 348-354.