Multidimensional GPCR profiling and screening using impedance-based label-free and real-time assay

G Protein-Coupled Receptor Screening Assays: Methods and Protocols

Volumn , Issue , 2015, Pages 215-226

  Ke, Ning ^a   Nguyen, Khanh ^a   Irelan, Jeffery ^a   Abassi, Yama A ^a  

^a ACEA Biosciences Inc   (United States)

Author keywords

GPCR; High throughput screening; Impedance based sensors

Indexed keywords

EID: 84921769016     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-1-4939-2336-6_15     Document Type: Chapter

References (6)

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3. Yu N et al (2006) Real-time monitoring of morphological changes in living cells by electronic cell sensor arrays: an approach to study G proteincoupled receptors. Anal Chem 78:35-43
4. Stallaert W et al (2012) Impedance responses reveal beta(2)-adrenergic receptor signaling pluridimensionality and allow classification of ligands with distinct signaling profiles. PLoS One 7:e29420
5. Gibson SK, Gilman AG (2006) Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors. Proc Natl Acad Sci U S A 103:212-217
6. Zhang J-H, Chung TDY, Oldenburg KR (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4:67-73