BONCAT: Metabolic labeling, click chemistry, and affinity purification of newly synthesized proteomes

Methods in Molecular Biology

Volumn 1266, Issue , 2015, Pages 199-215

  Landgraf, Peter ^a   Antileo, Elmer R ^a   Schuman, Erin M ^a   Dieterich, Daniela C ^a  

^a NONE

Author keywords

Affinity purification; Click chemistry; Mass spectrometry; Protein synthesis; Proteome

Indexed keywords

AMINO ACID; COPPER; HOMOPROPARGYLGLYCINE; METHIONINE; PROTEOME; UNCLASSIFIED DRUG; ALANINE; AZIDOHOMOALANINE;

AFFINITY PURIFICATION; ANIMAL CELL; ARTICLE; BRAIN; CATALYST; CELL CULTURE; CELL FRACTIONATION; CELL PROLIFERATION; CELL VIABILITY; CLICK CHEMISTRY; CONTROLLED STUDY; CYCLOADDITION; HEK293 CELL LINE; ISOTOPE LABELING; MASS SPECTROMETRY; MOLECULAR BIOLOGY; NERVE CELL; NERVE CELL CULTURE; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN HOMEOSTASIS; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RAT; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; STAINING;

MAMMALIA;

ALANINE; CHROMATOGRAPHY, AFFINITY; CLICK CHEMISTRY; CYCLOADDITION REACTION; HEK293 CELLS; HUMANS; PROTEOME; STAINING AND LABELING;

EID: 84921759405     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2272-7_14     Document Type: Article

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