Purification of specific chromatin loci for proteomic analysis

Methods in Molecular Biology

Volumn 1228, Issue , 2015, Pages 83-92

  Byrum, Stephanie D ^a   Taverna, Sean D ^a   Tackett, Alan J ^a  

^a NONE

Author keywords

Chromatin; Epigenetics; Histone; Posttranslational modification; Proteomics; Purification

Indexed keywords

ARTICLE; BINDING AFFINITY; CHROMATIN AFFINITY PURIFICATION WITH MASS SPECTROMETRY; HISTONE MODIFICATION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEOMICS; YEAST; CELL CULTURE TECHNIQUE; CHROMATIN; CYTOLOGY; FRACTIONATION; GENE LOCUS; GENETICS; HOMOLOGOUS RECOMBINATION; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; SACCHAROMYCES CEREVISIAE;

CHROMATIN; FORMALDEHYDE; GAL1 PROTEIN, S CEREVISIAE; GALACTOKINASE; SACCHAROMYCES CEREVISIAE PROTEIN;

CELL CULTURE TECHNIQUES; CHEMICAL FRACTIONATION; CHROMATIN; FORMALDEHYDE; GALACTOKINASE; GENETIC LOCI; HOMOLOGOUS RECOMBINATION; MASS SPECTROMETRY; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOMICS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84921712064     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1680-1_8     Document Type: Article

References (10)

1. Dedon PC, Soults JA, Allis CD, et al (1991) Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes. Mol Cell Biol 11:1729–1733
2. Ren B, Robert F, Wyrick JJ, et al (2000) Genome-wide location and function of DNA binding proteins. Science 290:2306–2309
3. Pokholok DK, Harbison CT, Levine S, et al (2005) Genome-wide map of nucleosome acetylation and methylation in yeast. Cell 122:517–527
4. Robertson G, Hirst M, Bainbridge M, et al (2007) Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing. Nat Methods 4:651–657
5. Johnson DS, Mortazavi A, Myers RM, et al (2007) Genome-wide mapping of in vivo protein- DNA interactions. Science 316:1497–1502
6. Barski A, Cuddapah S, Cui K, et al (2007) High-resolution profiling of histone methylations in the human genome. Cell 129: 823–837
7. Mikkelsen TS, Ku M, Jaffe DB, et al (2007) Genome-wide maps of chromatin state in pluripotent and lineage-committed cells. Nature 448:553–560
8. Byrum S, Raman A, Taverna SD, et al (2012) ChAP-MS: a method for identification of proteins and histone posttranslational modifications at a single genomic locus. Cell Rep 2: 198–205
9. Byrum SD, Smart SK, Larson S, et al (2012) Analysis of stable and transient protein-protein interactions. Methods Mol Biol 833:143–152
10. Byrum SD, Taverna SD, Tackett AJ, (2011) Quantitative analysis of histone exchange for transcriptionally active chromatin. J Clin Bioinform 1:17