Vibratome sectioning mouse retina to prepare photoreceptor cultures

Journal of Visualized Experiments

Volumn , Issue 94, 2014, Pages

  Clérin, Emmanuelle ^a,b,c,d   Yang, Ying ^a,b,c,d   Forster, Valérie ^a,b,c,d   Fontaine, Valérie ^a,b,c,d   Sahel, José Alain ^b,c,d   Léveillard, Thierry ^a,b,c,d  

^a Institut de la Vision   (France)

^b SORBONNE UNIVERSITÉ   (France)

^c INSERM   (France)

^d CNRS   (France)

Author keywords

Flat mounted mouse retina; Inherited retinal degeneration; Issue 94; Neuroscience; Primary photoreceptor cell culture; Rod derived cone viability factor; S Antigen; Transplantation

Indexed keywords

EID: 84921634296     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51954     Document Type: Article

References (28)

1. The Organization of the Retina and Visual System. Webvision. Kolb, H., Nelson, R., Fernandez, E., Jones, B. (2014).
2. Perez De Sevilla Muller, L., Shelley, J., Weiler, R. Displaced amacrine cells of the mouse retina. J Comp Neurol. 505, (2), 177-189 (2007).
3. Mohand-Said, S., et al. Photoreceptor transplants increase host cone survival in the retinal degeneration (rd) mouse. Ophthalmic Res. 29, (5), 290-297 (1997).
4. Daiger, S., Sullivan, L., Bowne, S. RetNet, the Retinal Information Network. The University of Texas Health Science Center Houston, TX Available from: https://sph.uth.edu/retnet (2014).
5. Mohand-Said, S., Hicks, D., Dreyfus, H., Sahel, J. A. Selective transplantation of rods delays cone loss in a retinitis pigmentosa model. Arch Ophthalmol. 118, (6), 807-811 (2000).
6. MacLaren, R. E., et al. Retinal repair by transplantation of photoreceptor precursors. Nature. 444, (7116), 203-207 (2006).
7. Yang, Y., et al. Transplantation of photoreceptor and total neural retina preserves cone function in P23H rhodopsin transgenic rat. PLoS One. 5, (10), (2010).
8. Mohand-Said, S., et al. Normal retina releases a diffusible factor stimulating cone survival in the retinal degeneration mouse. Proc Natl Acad Sci USA. 95, (14), 8357-8362 (1998).
9. Fintz, A. C., et al. Partial characterization of retina-derived cone neuroprotection in two culture models of photoreceptor degeneration. Invest Ophthalmol Vis Sci. 44, (2), 818-825 (2003).
10. Cronin, T., Leveillard, T., Sahel, J. A. Retinal degenerations: from cell signaling to cell therapy; pre-clinical and clinical issues. Curr Gene Ther. 7, (2), 121-129 (2007).
11. Wright, A. F. A searchlight through the fog. Nat Genet. 17, (2), 132-134 (1997).
12. Leveillard, T., et al. Identification and characterization of rod-derived cone viability factor. Nat Genet. 36, (7), 755-759 (2004).
13. Lillig, C. H., Holmgren, A. Thioredoxin and related molecules--from biology to health and disease. Antioxid Redox Signal. 9, (1), 25-47 (2007).
14. Fridlich, R., et al. The thioredoxin-like protein rod-derived cone viability factor (RdCVFL) interacts with TAU and inhibits its phosphorylation in the retina. Mol Cell Proteomics. 8, (6), 1206-1218 (2009).
15. Yang, Y., et al. Functional cone rescue by RdCVF protein in a dominant model of retinitis pigmentosa. Mol Ther. 17, (5), 787-795 (2009).
16. Bennett, J. Strategies for delivery of rod-derived cone viability factor. Retina. 25, (8 Suppl), S47(2005).
17. Komaromy, A. M., et al. Transient photoreceptor deconstruction by CNTF enhances rAAV-mediated cone functional rescue in late stage CNGB3-achromatopsia. Mol Ther. 21, (6), 1131-1141 (2013).
18. Birch, D. G., Weleber, R. G., Duncan, J. L., Jaffe, G. J., Tao, W. Randomized trial of ciliary neurotrophic factor delivered by encapsulated cell intraocular implants for retinitis pigmentosa. Am J Ophthalmol. 156, (2), 283-292 (2013).
19. Cronin, T., et al. The disruption of the rod-derived cone viability gene leads to photoreceptor dysfunction and susceptibility to oxidative stress. Cell Death Differ. 17, (7), 1199-1210 (2010).
20. Leveillard, T., Sahel, J. A. Rod-derived cone viability factor for treating blinding diseases: from clinic to redox signaling. Sci Transl Med. 2, (26), 26ps16(2010).
21. Fontaine, V., Hicks, D., Dreyfus, H. Changes in ganglioside composition of photoreceptors during postnatal maturation of the rat retina. Glycobiology. 8, (2), 183-190 (1998).
22. Fontaine, V., Kinkl, N., Sahel, J., Dreyfus, H., Hicks, D. Survival of purified rat photoreceptors in vitro is stimulated directly by fibroblast growth factor-2. J Neurosci. 18, (23), 9662-9672 (1998).
23. Reichman, S., et al. The homeobox gene CHX10/VSX2 regulates RdCVF promoter activity in the inner retina. Hum Mol Genet. 19, (2), 250-261 (2010).
24. Sandu, C., Hicks, D., Felder-Schmittbuhl, M. P. Rat photoreceptor circadian oscillator strongly relies on lighting conditions. Eur J Neurosci. 34, (3), 507-516 (2011).
25. Dorrell, M. I., Aguilar, E., Weber, C., Friedlander, M. Global gene expression analysis of the developing postnatal mouse retina. Invest Ophthalmol Vis Sci. 45, (3), 1009-1019 (2004).
26. Brooks, M. J., Rajasimha, H. K., Roger, J. E., Swaroop, A. Next-generation sequencing facilitates quantitative analysis of wild-type and Nrl(-/-) retinal transcriptomes. Mol Vis. 17, 3034-3054 (2011).
27. Delyfer, M. N., et al. Transcriptomic analysis of human retinal surgical specimens using jouRNAI. J Vis Exp. (78), (2013).
28. Ying, S., et al. A CAT reporter construct containing 277bp GNAT2 promoter and 214bp IRBP enhancer is specifically expressed by cone photoreceptor cells in transgenic mice. Curr Eye Res. 17, (8), 777-782 (1998).