A CAT reporter construct containing 277bp GNAT2 promoter and 214bp IRBP enhancer is specifically expressed by cone photoreceptor cells in transgenic mice

Current Eye Research

Volumn 17, Issue 8, 1998, Pages 777-782

  Ying, Saixia ^a   Fong, Shao Ling ^b   Fong, Wei Bao ^b   Kao, Candace W C ^a   Converse, Richard L ^a   Kao, Winston W Y ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Chloramphenicol acetyltransferase (CAT); Cone cells; Human cone transducin subunit (GNAT2); Promoter; Transgenic mice

Indexed keywords

CHLORAMPHENICOL ACETYLTRANSFERASE; CYCLIC GMP; PHOSPHODIESTERASE; PROTEIN SUBUNIT; RETINOID BINDING PROTEIN; TRANSDUCIN; VISUAL PIGMENT;

ANIMAL TISSUE; ARTICLE; CHIMERA; CONTROLLED STUDY; DNA FLANKING REGION; ENHANCER REGION; ENZYME ACTIVATION; GENE EXPRESSION; IMMUNOHISTOCHEMISTRY; MOLECULAR CLONING; MOUSE; NONHUMAN; PHOTORECEPTOR CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; RETINA; RETINA CONE; SOUTHERN BLOTTING; TISSUE DISTRIBUTION; TISSUE SPECIFICITY; TRANSGENIC MOUSE;

ANIMALS; BLOTTING, SOUTHERN; CHLORAMPHENICOL O-ACETYLTRANSFERASE; CONES (RETINA); DNA PRIMERS; ENHANCER ELEMENTS (GENETICS); EYE PROTEINS; GENE EXPRESSION; GENES, REPORTER; IMMUNOENZYME TECHNIQUES; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RETINOL-BINDING PROTEINS; TISSUE DISTRIBUTION; TRANSDUCIN;

EID: 0031814447     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/ceyr.17.8.777.5158     Document Type: Article

References (15)

1. Stryer L. Visual excitation and recovery. J Biol Chem. 1991;266:10711-10714.
2. Spickofsky N, Robichon A, Danho W, et al. Biochemical analysis of the transducin-phosphodiesterase interaction. Struc Biol. 1994;1:771-781.
3. Yau KW. Phototransduction mechanism in retinal rods and cones: the Friedenwald lecture. Invest Ophthalmol Vis Sci. 1994;35:9-32.
4. Lolley RN, Lee R H. Cyclic GMP and photoreceptor function. FASEB J. 1990;4:3001-3008.
5. Nathans J, Hogness DS. Isolation and neucleotide sequence of the gene encoding human rhodopsin. Proc Natl Acad Sci USA. 1984;81:4851-4855.
6. Nathans J, Thomas D, Hogness DS. Molecular genetics of human color vision: the genes encoding blue, green and red pigments. Science. 1986;232:193-202.
7. Lerea CL, Somers DE, Hurley JB, Klock IB, Bunt-Milam AH. Identification of specific transducin alpha subunits in retinal rod and cone photoreceptors. Science. 1986;234:77-80.
8. Gillespie PG, Beavo JA. Characterization of a bovine cone photoreceptor phosphodiesterase purified by cyclic GMP-sepharose chromatography. J Biol Chem. 1988;263:8133-8141.
9. Fong SL. Charaterization of the human rod transducin alpha-subunit gene. Nucl Acids Res. 1992;20:2865-2870.
10. Morris TA, Fong SL. Characterization of the gene encoding human cone transducin alpha-subunit (GNAT2). Genomics. 1993;17:442-448.
11. Morris TA, Fong WB, Ward MJ, Hu H, Fong SL. Localization of upstream silencer elements involved in the expression of cone transducin α-subunit (GNAT2). Invest Ophthal Vis Sci. 1997;38:196-206.
12. Fong SL, Morris TA, Lee CH, Donoso LA, Fong WB. Transfection of retinoblastoma cells: localization of cis-acting elements for interstitial retinoid-binding protein gene. Meth Neurosci. 1993;15:322-330.
13. Fong SL, Fong WB, Morris TA, Kedzie KM, Bridges CD. Characterization and comparative structural features of the gene for human interstitial retinoi-binding protein. J Biol Chem. 1990;265:3648-3653.
14. Zigman JM, Westermark GT, LaMendola J, Steiner DF. Expression of cone transducin, Gza, and other G-protein α-subunit messenger ribonucleic acids in pancreatic islets. Endocrinology. 1994;135:31-37.
15. Carter-Dawson LD, LaVail MM. Rods and cones in the mouse retina. J Comp Neurol. 1979;188:245-262.