Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders

Molecular Biotechnology

Volumn 57, Issue 2, 2015, Pages 155-159

  Panero, Julieta ^a   O’Callaghan, Nathan J ^b   Fenech, Michael ^b   Slavutsky, Irma ^a  

^a ACADEMIA NACIONAL DE MEDICINA   (Argentina)

^b CSIRO   (Australia)

Author keywords

Absolute quantification; Bone marrow samples; MGUS; Multiple myeloma; Telomere length

Indexed keywords

BONE; CHROMOSOMES; DIAGNOSIS; MAMMALS;

ABSOLUTE QUANTIFICATION; BONE MARROW; MGUS; MULTIPLE MYELOMA; TELOMERE LENGTH;

POLYMERASE CHAIN REACTION;

BETA 2 MICROGLOBULIN; DNA; GENOMIC DNA; HEMOGLOBIN;

ABSOLUTE QUANTITATIVE POLYMERASE CHAIN REACTION; ADULT; AGED; ARTICLE; BONE MARROW; CLINICAL ARTICLE; CLINICAL FEATURE; CONTROLLED STUDY; DNA DETERMINATION; FEMALE; HEMATOLOGIC DISEASE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MALE; MONOCLONAL IMMUNOGLOBULINEMIA; MULTIPLE MYELOMA; POLYMERASE CHAIN REACTION; PROGNOSIS; QUANTITATIVE ANALYSIS; RESTRICTION FRAGMENT; TELOMERE HOMEOSTASIS; TELOMERE SHORTENING; TERMINAL RESTRICTION FRAGMENT; VALIDATION STUDY; BONE MARROW CELL; DISEASE COURSE; GENETICS; HEMATOLOGIC NEOPLASMS; PATHOLOGY; PLASMA CELL; TELOMERE;

MAMMALIA;

BONE MARROW CELLS; DISEASE PROGRESSION; HEMATOLOGIC NEOPLASMS; HUMANS; PLASMA CELLS; POLYMERASE CHAIN REACTION; TELOMERE; TELOMERE HOMEOSTASIS;

EID: 84921355093     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-014-9811-8     Document Type: Article

References (15)

1. Counter, C. M., Avilion, A. A., LeFeuvre, C. E., et al. (1992). Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO Journal,11, 1921–1929.
2. Wu, X., Amos, C. I., Zhu, Y., et al. (2003). Telomere dysfunction: A potential cancer predisposition factor. Journal of the National Cancer Institute,95, 1211–1218.
3. Hahn, W. C. (2003). Role of telomeres and telomerase in the pathogenesis of human cancer. Journal of Clinical Oncology,21, 2034–2043.
4. Stewart, S. A., & Weinberg, R. A. (2006). Telomeres: Cancer to human aging. Annual Review of Cell and Developmental Biology,22, 531–557.
5. Allshire, R. C., Dempster, M., & Hastie, N. D. (1989). Human telomeres contain at least three types of G-rich repeat distributed non-randomly. Nucleic Acids Research,17, 4611–4627.
6. Cawthon, R. M. (2002). Telomere measurement by quantitative PCR. Nucleic Acids Research,30, e47.
7. O’Callaghan, N., Dhillon, V., Thomas, P., & Fenech, M. (2008). A quantitative real-time PCR method for absolute telomere length. BioTechniques,44, 807–809.
8. O’Callaghan, N., & Fenech, M. (2011). A quantitative PCR method for measuring absolute telomere length. Biological Procedures Online,13, 3.
9. Folini, M., Venturini, L., Cimino-Reale, G., & Zaffaroni, N. (2011). Telomeres as targets for anticancer therapies. Expert Opinion on Therapeutic Targets,15, 579–593.
10. Greipp, P. R., San Miguel, J., Durie, B. G., et al. (2005). International staging system for multiple myeloma. Journal of Clinical Oncology,23, 3412–3420.
11. Cottliar, A., Pedrazzini, E., Corrado, C., Engelberger, M. I., Narbaitz, M., & Slavutsky, I. (2003). Telomere shortening in patients with plasma cell disorders. European Journal of Haematology,71, 334–340.
12. Baird, D. M. (2005). New developments in telomere length analysis. Experimental Gerontology,40, 363–368.
13. Wu, K. D., Orme, L. M., Shaughnessy, J, Jr, Jacobson, J., Barlogie, B., & Moore, M. A. (2003). Telomerase and telomere length in multiple myeloma: Correlations with disease heterogeneity, cytogenetic status, and overall survival. Blood,101, 4982–4989.
14. Panero, J., Arbelbide, J., Fantl, D., Rivello, H. G., Kohan, D., & Slavutsky, I. (2010). Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma. Molecular Medicine,16, 471–478.
15. Jones, C. H., Pepper, C., & Baird, D. M. (2012). Telomere dysfunction and its role in haematological cancer. British Journal of Haematology,156, 573–587.