Chemical synthesis of a pore-forming antimicrobial protein, caenopore-5, by using native chemical ligation at a Glu-Cys site

ChemBioChem

Volumn 16, Issue 2, 2015, Pages 328-336

  Medini, Karima ^a   Harris, Paul W R ^a,b   Hards, Kiel ^c   Dingley, Andrew J ^d   Cook, Gregory M ^c   Brimble, Margaret A ^a,b  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

^c UNIVERSITY OF OTAGO   (New Zealand)

^d FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Antibiotics; Antimicrobial protein; Caenopore; Native chemical ligation; Protein design; Protein folding

Indexed keywords

CAENOPORE 5; CYSTEINE; GLUTATHIONE; PEPTIDE DERIVATIVE; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; CAENORHABDITIS ELEGANS PROTEIN; RECOMBINANT PROTEIN;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE PERMEABILITY; CHEMICAL LIGATION; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; DESULFURIZATION; DISULFIDE BOND; DRUG PURIFICATION; DRUG SYNTHESIS; ELUTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY; MEMBRANE VESICLE; OXIDATION REDUCTION REACTION; PEPTIDE SYNTHESIS; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; RING OPENING; SOLID PHASE SYNTHESIS; STRUCTURE ACTIVITY RELATION; ANIMAL; CAENORHABDITIS ELEGANS; CELL MEMBRANE; CHEMISTRY; DRUG EFFECTS; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PERMEABILITY; SYNTHESIS;

CAENORHABDITIS ELEGANS;

AMINO ACID SEQUENCE; ANIMALS; ANTI-INFECTIVE AGENTS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CELL MEMBRANE; CHEMISTRY TECHNIQUES, SYNTHETIC; CIRCULAR DICHROISM; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; PERMEABILITY; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SOLID-PHASE SYNTHESIS TECHNIQUES;

EID: 84920837400     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201402513     Document Type: Article

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