Comparison of pulsed-field gel electrophoresis & repetitive sequence-based PCR methods for molecular epidemiological studies of Escherichia coli clinical isolates

Indian Journal of Medical Research

Volumn 140, Issue , 2014, Pages 679-685

  Bae, Il Kwon ^a   Kim, Juwon ^b   Sun, Je Young Hannah ^c   Jeong, Seok Hoon ^b   Kim, Yong Rok ^d   Wang, Kang Kyun ^d   Lee, Kyungwon ^b  

^a Silla University   (South Korea)

^b Yonsei University College of Medicine   (South Korea)

^c RICE UNIVERSITY   (United States)

^d Yonsei University   (South Korea)

Author keywords

Escherichia coli; Genetic relationship; MLST; PFGE; Rep PCR; Sequence type

Indexed keywords

ACHTMANS MLST SCHEME; ARTICLE; BETA LACTAMASE AMPC PRODUCING ESCHERICHIA COLI; COMPARATIVE STUDY; DNA FINGERPRINTING; ESCHERICHIA COLI; EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI; GENOTYPING TECHNIQUE; MOLECULAR PHYLOGENY; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PHYLOGENETIC TREE; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; REPETITIVE SEQUENCE BASED POLYMERASE CHAIN REACTION; WHITTAMS MLST SCHEME; ENZYMOLOGY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR EPIDEMIOLOGY; PATHOGENICITY; PROCEDURES;

AMPC BETA-LACTAMASES; BACTERIAL PROTEIN; BETA LACTAMASE;

BACTERIAL PROTEINS; BETA-LACTAMASES; ELECTROPHORESIS, GEL, PULSED-FIELD; ESCHERICHIA COLI; HUMANS; MOLECULAR EPIDEMIOLOGY; POLYMERASE CHAIN REACTION;

EID: 84920450178     PISSN: 09715916     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Horner C, Fawley W, Morris K, Denton M, Wilcox M. Escherichia coli bacteremia: 2 years of prospective regional surveillance (2010-12). J Antimicrob Chemother 2013; 69: 91-100.
2. Wang G, Huang T, Surendraiah PK, Wang K, Komal R, Zhuge J, et al. CTX-M β-lactamase-producing Klebsiella pneumoniae in Suburban New York City, New York, USA. Emerg Infect Dis 2013; 19: 1803-10.
3. Hawser SP, Badal RE, Bouchillon SK, Hoban DJ, Hackel MA, Biedenbach DJ, et al. Susceptibility of Gram-negative aerobic bacilli from intra-abdominal pathogens to antimicrobial agents collected in the United States during 2011. J Infect 2013; 68: 71-6.
4. Wirth T, Falush D, Lan R, Colles F, Mensa P, Wieler LH, et al. Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol 2006; 60: 1136-51.
5. Brolund A, Hæggman S, Edguist PJ, Gezelius L, Olsson-Liljequist B, Wisell KT, et al. The Diversilab system versus pulsed-field gel electrophoresis: characterisation of extended-spectrum β-lactamase producing Escherichia coli bAE et al: CoMPARISoN oF PFGE & REP-PCK FoR GENETIC STUDIES IN E. CoLI and Klebsiella pneumoniae. J Microbiol Methods 2010; 83: 224-30.
6. Ross TL, Merz WG, Farkosh M, Carroll KC. Comparison of an automated repetitive sequence-based PCR microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2005; 43: 5642-7.
7. Pitout JD, Campbell L, Church DL, Wang PW, Guttman DS, Gregson Db. Using a commercial DiversiLab semiautomated repetitive sequence-based PCR typing technique for identification of Escherichia coli clone ST131 producing CTX-M-15. J Clin Microbiol 2009; 47: 1212-5.
8. Ryoo NH, Kim EC, Hong SG, Park YJ, Lee K, bae IK, et al. Dissemination of SHv-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. J Antimicrob Chemother 2005; 56: 698-702.
9. Song W, Jeong SH, Kim JS, Kim HS, Shin DH, Rho KH, et al. Use of boronic acid disk methods to detect the combined expression of plasmid-mediated AmpC β-lactamases and extended-spectrum β-lactamases in clinical isolates of Klebsiella spp., Salmonella spp., and Proteus mirabilis. Diagn Microbiol Infect Dis 2007; 57: 315-8.
10. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-9.
11. Doi Y, Paterson DL, Egea P, Pascual A, López-Cerero L, Navarro MD, et al. Extened-spectrum and CMY-type β-lactamase producing Escherichia coli in clinical samples and retail meat from Pittsburgh, USA and Seville, Spain. Clin Microbiol Infect 2010; 16: 33-8.
12. Hunter PA, Dawson S, French GL, Goossens H, Hawkey PA, Kuijper EJ, et al. Antimicrobial-resistant pathogens in animals and man: prescribing, practices and policies. J Antimicrob Chemother 2010; 65 (Suppl 1): i3-7.
13. Steinsland H, Lacher DW, Sommerfelt H, Whittam TS. Ancestral lineages of human enterotoxigenic Escherichia coli. J Clin Microbiol 2010; 48: 2916-24.
14. Valverde A, Cantón R, Garcillán-barcia MP, Novais A, Galán JC, Alvarado A, et al. Spread of blaCTX-M-14 is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, b1, and D in Spain. Antimicrob Agents Chemother 2009; 53: 5204-1