Quantification of filamentation by uropathogenic Escherichia coli during experimental bladder cell infection by using semi-automated image analysis

Journal of Microbiological Methods

Volumn 109, Issue , 2015, Pages 110-116

  Klein, Kasper ^a   Palarasah, Yaseelan ^a   Kolmos, Hans Jørn ^a   Møller Jensen, Jakob ^a   Andersen, Thomas Emil ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Bacterial filamentation; Cystitis; Escherichia coli; In vitro infection; Uropathogen

Indexed keywords

ARTICLE; AUTOMATION; BACTERIAL CELL; BACTERIAL COLONIZATION; BACTERIAL FILAMENTATION; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIAL STRUCTURES; BACTERIUM DETECTION; CELL PROLIFERATION; CONTROLLED STUDY; CYSTITIS; EXPERIMENTAL INFECTION; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IN VITRO STUDY; MICTURITION; NONHUMAN; SHEAR STRESS; SODIUM URINE LEVEL; UROPATHOGENIC ESCHERICHIA COLI; BLADDER; CYTOLOGY; FLUORESCENCE IMAGING; GROWTH, DEVELOPMENT AND AGING; HUMAN; IMAGE PROCESSING; MICROBIOLOGY; PROCEDURES; UROTHELIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; SALMONELLA;

HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; OPTICAL IMAGING; URINARY BLADDER; UROPATHOGENIC ESCHERICHIA COLI; UROTHELIUM;

EID: 84920111249     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.12.017     Document Type: Article

References (28)

1. Andersen T.E., Kingshott P., Palarasah Y., Benter M., Alei M., Kolmos H.J. A flow chamber assay for quantitative evaluation of bacterial surface colonization used to investigate the influence of temperature and surface hydrophilicity on the biofilm forming capacity of uropathogenic Escherichia coli. J. Microbiol. Methods 2010, 81:135-140.
2. Andersen T.E., Khandige S., Madelung M., Brewer J., Kolmos H.J., Moller-Jensen J. Escherichia coli uropathogenesis in vitro: invasion, cellular escape, and secondary infection analyzed in a human bladder cell infection model. Infect. Immun. 2012, 80:1858-1867.
3. Berry R.E., Klumpp D.J., Schaeffer A.J. Urothelial cultures support intracellular bacterial community formation by uropathogenic Escherichia coli. Infect. Immun. 2009, 77:2762-2772.
4. Brooks T., Keevil C.W. A simple artificial urine for the growth of urinary pathogens. Lett. Appl. Microbiol. 1997, 24:203-206.
5. Chauhan A., Madiraju M.V., Fol M., Lofton H., Maloney E., Reynolds R., Rajagopalan M. Mycobacterium tuberculosis cells growing in macrophages are filamentous and deficient in FtsZ rings. J. Bacteriol. 2006, 188:1856-1865.
6. Corno G., Jurgens K. Direct and indirect effects of protist predation on population size structure of a bacterial strain with high phenotypic plasticity. Appl. Environ. Microbiol. 2006, 72:78-86.
7. D'Ari R. The SOS system. Biochimie 1985, 67:343-347.
8. Eriksson S., Lucchini S., Thompson A., Rhen M., Hinton J.C. Unravelling the biology of macrophage infection by gene expression profiling of intracellular Salmonella enterica. Mol. Microbiol. 2003, 47:103-118.
9. Hahn M.W., Moore E.R., Hofle M.G. Bacterial filament formation, a defense mechanism against flagellate grazing, is growth rate controlled in bacteria of different phyla. Appl. Environ. Microbiol. 1999, 65:25-35.
10. Henry T., Garcia-Del P.F., Gorvel J.P. Identification of Salmonella functions critical for bacterial cell division within eukaryotic cells. Mol. Microbiol. 2005, 56:252-267.
11. Horvath D.J., Li B., Casper T., Partida-Sanchez S., Hunstad D.A., Hultgren S.J., Justice S.S. Morphological plasticity promotes resistance to phagocyte killing of uropathogenic Escherichia coli. Microbes Infect. 2011, 13:426-437.
12. Humphrey S., Macvicar T., Stevenson A., Roberts M., Humphrey T.J., Jepson M.A. SulA-induced filamentation in Salmonella enterica serovar Typhimurium: effects on SPI-1 expression and epithelial infection. J. Appl. Microbiol. 2011, 111:185-196.
13. Justice S.S., Hung C., Theriot J.A., Fletcher D.A., Anderson G.G., Footer M.J., Hultgren S.J. Differentiation and developmental pathways of uropathogenic Escherichia coli in urinary tract pathogenesis. Proc. Natl. Acad. Sci. U. S. A. 2004, 101:1333-1338.
14. Justice S.S., Hunstad D.A., Seed P.C., Hultgren S.J. Filamentation by Escherichia coli subverts innate defenses during urinary tract infection. Proc. Natl. Acad. Sci. U. S. A. 2006, 103:19884-19889.
15. Justice S.S., Hunstad D.A., Cegelski L., Hultgren S.J. Morphological plasticity as a bacterial survival strategy. Nat. Rev. Microbiol. 2008, 6:162-168.
16. Kau A.L., Hunstad D.A., Hultgren S.J. Interaction of uropathogenic Escherichia coli with host uroepithelium. Curr. Opin. Microbiol. 2005, 8:54-59.
17. Lorian V., Ernst J., Amaral L. The post-antibiotic effect defined by bacterial morphology. J. Antimicrob. Chemother. 1989, 23:485-491.
18. Mattick K.L., Jorgensen F., Legan J.D., Cole M.B., Porter J., Lappin-Scott H.M., Humphrey T.J. Survival and filamentation of Salmonella enterica serovar Enteritidis PT4 and Salmonella enterica serovar Typhimurium DT104 at low water activity. Appl. Environ. Microbiol. 2000, 66:1274-1279.
19. Mattick K.L., Rowbury R.J., Humphrey T.J. Morphological changes to Escherichia coli O157:H7, commensal E. coli and Salmonella spp in response to marginal growth conditions, with special reference to mildly stressing temperatures. Sci. Prog. 2003, 86:103-113.
20. Mulvey M.A., Schilling J.D., Hultgren S.J. Establishment of a persistent Escherichia coli reservoir during the acute phase of a bladder infection. Infect. Immun. 2001, 69:4572-4579.
21. Pernthaler J., Posch T., Simek K., Vrba J., Amann R., Psenner R. Contrasting bacterial strategies to coexist with a flagellate predator in an experimental microbial assemblage. Appl. Environ. Microbiol. 1997, 63:596-601.
22. Rosen D.A., Hooton T.M., Stamm W.E., Humphrey P.A., Hultgren S.J. Detection of intracellular bacterial communities in human urinary tract infection. PLoS Med. 2007, 4:e329.
23. Rosenberger C.M., Finlay B.B. Macrophages inhibit Salmonella typhimurium replication through MEK/ERK kinase and phagocyte NADPH oxidase activities. J. Biol. Chem. 2002, 277:18753-18762.
24. Rosenberger C.M., Gallo R.L., Finlay B.B. Interplay between antibacterial effectors: a macrophage antimicrobial peptide impairs intracellular Salmonella replication. Proc. Natl. Acad. Sci. U. S. A. 2004, 101:2422-2427.
25. Scholz O., Thiel A., Hillen W., Niederweis M. Quantitative analysis of gene expression with an improved green fluorescent protein. p6. Eur. J. Biochem. 2000, 267:1565-1570.
26. Stackhouse R.R., Faith N.G., Kaspar C.W., Czuprynski C.J., Wong A.C. Survival and virulence of Salmonella enterica serovar Enteritidis filaments induced by reduced water activity. Appl. Environ. Microbiol. 2012, 78:2213-2220.
27. Weiser J.N. The battle with the host over microbial size. Curr. Opin. Microbiol. 2013, 16:59-62.
28. Young K.D. The selective value of bacterial shape. Microbiol. Mol. Biol. Rev. 2006, 70:660-703.