Role of Antioxidants in Dermal Aging: An In Vitro Study by q-RT-PCR

Aesthetic Plastic Surgery

Volumn 38, Issue 5, 2014, Pages 1011-1016

  Avantaggiato, A ^a   Bertuzzi, G ^b   Vitiello, U ^b   Iannucci, G ^b   Pasin, M ^b   Pascali, M ^b   Cervelli, V ^b   Carinci, F ^a  

^a UNIVERSITY OF FERRARA   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

Antioxidant; Biostimulation; Fibroblasts; Gene expression; N Acetylcysteine

Indexed keywords

ACETYLCYSTEINE; AMINO ACID; AMINO-ACID, GLUCOSE, AND ELECTROLYTE SOLUTION; ANTIOXIDANT; ELECTROLYTE; GLUCOSE; PARENTERAL SOLUTION; SCAVENGER; SCLEROPROTEIN; SOLUTION AND SOLUBILITY;

ADMINISTRATION AND DOSAGE; CUTANEOUS PARAMETERS; DRUG EFFECTS; FIBROBLAST; GENE EXPRESSION; GENETICS; HUMAN; IN VITRO STUDY; INTRADERMAL DRUG ADMINISTRATION; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOLUTION AND SOLUBILITY;

ACETYLCYSTEINE; AMINO ACIDS; ANTIOXIDANTS; ELECTROLYTES; EXTRACELLULAR MATRIX PROTEINS; FIBROBLASTS; FREE RADICAL SCAVENGERS; GENE EXPRESSION; GLUCOSE; HUMANS; IN VITRO TECHNIQUES; INJECTIONS, INTRADERMAL; PARENTERAL NUTRITION SOLUTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SKIN AGING; SOLUTIONS;

EID: 84919345199     PISSN: 0364216X     EISSN: 14325241     Source Type: Journal    
DOI: 10.1007/s00266-014-0380-9     Document Type: Article

References (28)

1. Avantaggiato A, Palmieri A, Carinci F, Pasin M, Bertuzzi G (2013) Biostimulation and biorevitalization: effects on human skin fibroblasts. Ann Oral Maxillofac Surg 1(2):11
2. Bertuzzi G (2010) Medicina anti-aging. Academia Universa Press, Milan, pp 188–190
3. Black HS (1987) Potential involvement of free radical reactions in ultraviolet light-mediated cutaneous damage. Photochem Photobiol 46:213–221
4. Sohal RS, Weindruch R (1996) Oxidative stress, caloric restriction, and aging. Science 273:59–63
5. Kohen R, Gati I (2000) Skin low molecular weight antioxidants and their role in aging and in oxidative stress. Toxicology 148:149–157
6. Cadet J, Douki T (2011) Oxidatively generated damage to DNA by UVA radiation in cells and human skin. J Invest Dermatol 131:1005–1007
7. Gredilla R (2010) DNA damage and base excision repair in mitochondria and their role in aging. J Aging Res 2011:257093
8. D’Agostini F, Balansky RM, Camoirano A, De Flora S (2005) Modulation of light-induced skin tumors by N-acetylcysteine and/or ascorbic acid in hairless mice. Carcinogenesis 26:657–664
9. Kang S, Chung JH, Lee JH, Fisher GJ, Wan YS, Duell EA, Voorhees JJ (2003) Topical N-acetyl cysteine and genistein prevent ultraviolet-light-induced signaling that leads to photoaging in human skin in vivo. J Invest Dermatol 120:835–841
10. Alaluf S, Muir-Howie H, Hu HL, Evans A, Green MR (2000) Atmospheric oxygen accelerates the induction of a post-mitotic phenotype in human dermal fibroblasts: the key protective role of glutathione. Differentiation 66:147–155
11. De Flora S, Izzotti A, D’Agostini F, Balansky RM (2001) Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 22:999–1013
12. Sun L, Gu L, Wang S, Yuan J, Yang H, Zhu J, Zhang H (2012) N-acetylcysteine protects against apoptosis through modulation of group I metabotropic glutamate receptor activity. PLoS ONE 7(3):e32503
13. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402–408
14. Aruoma OI, Halliwell B, Hoey BM, Butler J (1989) The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med 6:593–597
15. Issels RD, Nagele A, Eckert KG, Wilmanns W (1988) Promotion of cystine uptake and its utilization for glutathione biosynthesis induced by cysteamine and N-acetylcysteine. Biochem Pharmacol 37:881–888
16. Morrison JP, Coleman MC, Aunan ES, Walsh SA, Spitz DR, Kregel KC (2005) Thiol supplementation in aged animals alters antioxidant enzyme activity after heat stress. J Appl Physiol 99:2271–2277
17. De La Fuente M, Victor VM (2000) Anti-oxidant as modulators of immune function. Immunol Cell Biol 7:999–1013
18. Monslow J, Williams JD, Norton N, Guy CA, Price IK, Coleman SL, Williams NM, Buckland PR, Spicer AP, Topley N, Davies M, Bowen T (2003) The human hyaluronan synthase genes: genomic structures, proximal promoters and polymorphic microsatellite markers. Int J Biochem Cell Biol 35:1272–1283
19. Laurent TC, Fraser JR (1992) Hyaluronan. FASEB J 6:2397–2404
20. Rosenbloom J (1984) Elastin: relation of protein and gene structure to disease. Lab Invest 51:605–623
21. Uitto J, Christiano AM, Kahari VM, Bashir MM, Rosenbloom J (1991) Molecular biology and pathology of human elastin. Biochem Soc Trans 19:824–829
22. Chua F, Laurent GJ (2006) Neutrophil elastase: mediator of extracellular matrix destruction and accumulation. Proc Am Thorac Soc 3:424–427
23. Vanderburg CR, Hay ED (1996) E-cadherin transforms embryonic corneal fibroblasts to stratified epithelium with desmosomes. Acta Anat (Basel) 157:87–104
24. Bowers SL, McFadden WA, Borg TK, Baudino TA (2012) Desmoplakin is important for proper cardiac cell–cell interactions. Microsc Microanal 18:107–114
25. Sawa Y, Kuroshima S, Yamaoka Y, Yoshida S (2005) Intracellular distribution of desmoplakin in human odontoblasts. J Histochem Cytochem 53:1099–1108
26. Quan T, He T, Kang S, Voorhees JJ, Fisher GJ (2004) Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol 165:741–751
27. Chang C, Hemmati-Brivanlou A (1999) Xenopus GDF6, a new antagonist of noggin and a partner of BMPs. Development 126:3347–3357
28. Banka S, Cain SA, Carim S, Daly SB, Urquhart JE, Erdem G, Harris J, Bottomley M, Donnai D, Kerr B, Kingston H, Kingston H, Superti-Furga A, Unger S, Ennis H, Worthington J, Worthington J, Worthington J, Herrick AL, Merry CL, Yue WW, Kielty CM, Newman WG (2014) Leri’s pleonosteosis, a congenital rheumatic disease, results from microduplication at 8q22.1 encompassing GDF6 and SDC2 and provides insight into systemic sclerosis pathogenesis. Ann Rheum Dis. doi:10.1136/annrheumdis-2013-204309