Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI sepsityper combined with the BD phoenix automated system

Journal of Medical Microbiology

Volumn 63, Issue , 2014, Pages 1590-1594

  Hazelton, Briony ^a   Thomas, Lee C ^a   Olma, Thomas ^a   Kok, Jen ^a,b,c   O′Sullivan, Matthew ^a,b   Chen, Sharon C A ^a,b   Iredell, Jonathan R ^a,b,c  

^a WESTMEAD HOSPITAL   (Australia)

^b WESTMEAD HOSPITAL   (Australia)

^c University of Sydney   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOGLYCOSIDE; BETA LACTAM ANTIBIOTIC; CEFEPIME; CEFOXITIN; NITROFURAN DERIVATIVE; NITROFURANTOIN; ANTIINFECTIVE AGENT;

ACCURACY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL CELL; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; BROTH DILUTION; CITROBACTER KOSERI; ENTEROBACTER AEROGENES; ESCHERICHIA COLI; GENOTYPE; GRAM NEGATIVE BACTERIUM; GRAM NEGATIVE SEPSIS; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; LABORATORY DEVICE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; PSEUDOMONAS AERUGINOSA; SERRATIA MARCESCENS; STENOTROPHOMONAS MALTOPHILIA; TURNAROUND TIME; BACTEREMIA; BACTERIA; BLOOD; DRUG EFFECTS; EVALUATION STUDY; HUMAN; LABORATORY AUTOMATION; MASS SPECTROMETRY; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; PROCEDURES; TIME;

ANTI-BACTERIAL AGENTS; AUTOMATION, LABORATORY; BACTEREMIA; BACTERIA; BLOOD; HUMANS; MICROBIAL SENSITIVITY TESTS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 84919342820     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.075580-0     Document Type: Article

References (25)

1. Barenfanger, J., Drake, C. & Kacich, G. (1999). Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J Clin Microbiol 37, 1415–1418.
2. Beekmann, S. E., Diekema, D. J., Chapin, K. C. & Doern, G. V. (2003). Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol 41, 3119–3125.
3. Bruker Daltonik (2012). Bruker Guide to MALDI Sample Preparation – MALDI Preparation Protocols. Revision 2. Bremen: Bruker Daltonik.
4. Buchan, B. W., Riebe, K. M. & Ledeboer, N. A. (2012). Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles. J Clin Microbiol 50, 346–352.
5. Clark, R. B., Lewinski, M. A., Loeffelholz, M. J. & Tibbetts, R. J. (2009). In Cumitech 31A, Verification and Validation of Procedures in the Clinical Microbiology Laboratory, pp. 13–14. Edited by S. E. Sharp. Washington, DC: ASM Press.
6. CLSI (2012). Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute.
7. Ellem, J., Partridge, S. R. & Iredell, J. R. (2011). Efficient direct extended-spectrum b-lactamase detection by multiplex real-time PCR: accurate assignment of phenotype by use of a limited set of genetic markers. J Clin Microbiol 49, 3074–3077.
8. Espedido, B. A., Partridge, S. R. & Iredell, J. R. (2008). Bla(IMP-4) in different genetic contexts in Enterobacteriaceae isolates from Australia. Antimicrob Agents Chemother 52, 2984–2987.
9. Gaieski, D. F., Mikkelsen, M. E., Band, R. A., Pines, J. M., Massone, R., Furia, F. F., Shofer, F. S. & Goyal, M. (2010). Impact of time to antibiotics on survival in patients with severe sepsis or septic shock in whom early goal-directed therapy was initiated in the emergency department. Crit Care Med 38, 1045–1053.
10. Ginn, A. N., Zong, Z., Wiklendt, A. M., Thomas, L. C., Merlino, J., Gottlieb, T., van Hal, S., Harkness, J., Macleod, C. & other authors (2013). Limited diversity in the gene pool allows prediction of third-generation cephalosporin and aminoglycoside resistance in Esche-richia coli and Klebsiella pneumoniae. Int J Antimicrob Agents 42, 19–26.
11. Gray, T. J., Thomas, L., Olma, T., Iredell, J. R. & Chen, S. C. (2013). Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry. Diagn Microbiol Infect Dis 77, 110–112.
12. Klein, S., Zimmermann, S., Kö hler, C., Mischnik, A., Alle, W. & Bode, K. A. (2012). Integration of matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry in blood culture diagnostics: a fast and effective approach. J Med Microbiol 61, 323–331.
13. Kok, J., Thomas, L. C., Olma, T., Chen, S. C. A. & Iredell, J. R. (2011). Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization SepsityperTM and time of flight mass spectrometry. PLoS ONE 6, e23285.
14. Kumar, A., Roberts, D., Wood, K. E., Light, B., Parrillo, J. E., Sharma, S., Suppes, R., Feinstein, D., Zanotti, S. & other authors (2006). Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34, 1589–1596.
15. Partridge, S. R., Ellem, J. A., Tetu, S. G., Zong, Z., Paulsen, I. T. & Iredell, J. R. (2011). Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-blaCTX-M-15 from an Escherichia coli ST131 isolate. Antimicrob Agents Chemother 55, 5933–5935.
16. Partridge, S. R., Ginn, A. N., Paulsen, I. T. & Iredell, J. R. (2012). pEl1573 carrying blaIMP-4, from Sydney, Australia, is closely related to other IncL/M plasmids. Antimicrob Agents Chemother 56, 6029–6032.
17. Shoma, S., Kamruzzaman, M., Ginn, A. N., Iredell, J. R. & Partridge, S. R. (2014). Characterization of multidrug-resistant Klebsiella pneumoniae from Australia carrying blaNDM-1. Diagn Microbiol Infect Dis 78, 93–97.
18. Shorr, A. F., Micek, S. T., Jackson, W. L., Jr & Kollef, M. H. (2007). Economic implications of an evidence-based sepsis protocol: can we improve outcomes and lower costs? Crit Care Med 35, 1257–1262.
19. Stevenson, L. G., Drake, S. K. & Murray, P. R. (2010). Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48, 444–447.
20. Valenzuela, J. K., Thomas, L., Partridge, S. R., van der Reijden, T., Dijkshoorn, L. & Iredell, J. (2007). Horizontal gene transfer in a polyclonal outbreak of carbapenem-resistant Acinetobacter bauman-nii. J Clin Microbiol 45, 453–460.
21. Wimmer, J. L., Long, S. W., Cernoch, P., Land, G. A., Davis, J. R., Musser, J. M. & Olsen, R. J. (2012). Strategy for rapid identification and antibiotic susceptibility testing of Gram-negative bacteria directly recovered from positive blood cultures using the Bruker MALDI Biotyper and the BD Phoenix system. J Clin Microbiol 50, 2452–2454.
22. Yan, Y., He, Y., Maier, T., Quinn, C., Shi, G., Li, H., Stratton, C. W., Kostrzewa, M. & Tang, Y. W. (2011). Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system. J Clin Microbiol 49, 2528–2532.
23. Yonetani, S., Okazaki, M., Araki, K., Makino, H., Fukugawa, Y., Okuyama, T., Ohnishi, H. & Watanabe, T. (2012). Direct inoculation method using BacT/ALERT 3D and BD Phoenix System allows rapid and accurate identification and susceptibility testing for both Gram-positive cocci and Gram-negative rods in aerobic blood cultures. Diagn Microbiol Infect Dis 73, 129–134.
24. Zong, Z., Partridge, S. R. & Iredell, J. R. (2008a). RmtC 16S rRNA methyltransferase in Australia. Antimicrob Agents Chemother 52, 794–795.
25. Zong, Z., Partridge, S. R., Thomas, L. & Iredell, J. R. (2008b). Dominance of blaCTX-M within an Australian extended-spectrum b-lactamase gene pool. Antimicrob Agents Chemother 52, 4198–4202.