Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samples

International Journal for Parasitology

Volumn 44, Issue 14, 2014, Pages 1105-1113

  Yang, Rongchang ^a   Paparini, Andrea ^a   Monis, Paul ^b   Ryan, Una ^a  

^a MURDOCH UNIVERSITY   (Australia)

^b AUSTRALIAN WATER QUALITY CENTRE   (Australia)

Author keywords

Cryptosporidium; Cryptosporidium oocysts; Droplet digital PCR (ddPCR); Molecular diagnostics; Parasites; Public health; Quantitative PCR (qPCR); Water

Indexed keywords

PROTOZOAL DNA; RNA 18S; ACTIN;

DNA; PARASITE; POLYMERASE CHAIN REACTION; PROTOZOAN; PUBLIC HEALTH; RNA;

ARTICLE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; COST EFFECTIVENESS ANALYSIS; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA DETERMINATION; DROPLET DIGITAL POLYMERASE CHAIN REACTION; FECES ANALYSIS; FLOW CYTOMETRY; GENE LOCUS; HUMAN; INTERMETHOD COMPARISON; LIMIT OF DETECTION; NONHUMAN; NUCLEIC ACID ANALYSIS; OOCYST; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; ANIMAL; BOVINE; CATTLE DISEASE; CHEMISTRY; CHI SQUARE DISTRIBUTION; COMPARATIVE STUDY; FECES; GENETICS; PARASITE IDENTIFICATION; PARASITOLOGY; PROCEDURES; REPRODUCIBILITY; SHEEP; SHEEP DISEASE; STANDARDS; VETERINARY;

ANIMALIA; BOS; CRYPTOSPORIDIUM; OVIS ARIES;

ACTINS; ANIMALS; CATTLE; CATTLE DISEASES; CHI-SQUARE DISTRIBUTION; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DNA, PROTOZOAN; FECES; HUMANS; OOCYSTS; PARASITE EGG COUNT; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SHEEP; SHEEP DISEASES;

EID: 84917744218     PISSN: 00207519     EISSN: 18790135     Source Type: Journal    
DOI: 10.1016/j.ijpara.2014.08.004     Document Type: Article

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