Gene cloning, sequence analysis, and expression profiles of a novel β-ring carotenoid hydroxylase gene from the photoheterotrophic green alga Chlorella kessleri

Molecular Biology Reports

Volumn 41, Issue 11, 2014, Pages 7103-7113

  Yu, Xiaona ^a   Cui, Hongli ^b,c   Cui, Yulin ^b   Wang, Yan ^b   Li, Xueqin ^d   Liu, Zhaopu ^a   Qin, Song ^a,b  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b YANTAI INSTITUTE OF COASTAL ZONE RESEARCH   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^d SHENZHEN UNIVERSITY   (China)

Author keywords

Carotenoid hydroxylase; Chlorellakessleri; Gene cloning; High light; Photoheterotrophic

Indexed keywords

ALPHA CAROTENE; AMINO ACID; BETA CAROTENE; CAROTENOID; COMPLEMENTARY DNA; CYTOCHROME P450; XANTHOPHYLL; ZEAXANTHIN; MIXED FUNCTION OXIDASE;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; CHLORELLA KESSLERI; CHLOROPLAST GENE; CKECYP97A1 GENE; CONTROLLED STUDY; DNA SEQUENCE; GENE; GENE AMPLIFICATION; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; GREEN ALGA; HETEROTROPHY; HYDROXYLATION; ISOELECTRIC POINT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYLOGENY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; CHLORELLA; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; LIGHT; METABOLISM; MOLECULAR GENETICS; NUCLEIC ACID AMPLIFICATION; RADIATION RESPONSE;

CHLOROPHYTA; PARACHLORELLA KESSLERI;

BASE SEQUENCE; CHLORELLA; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA, COMPLEMENTARY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, ENZYMOLOGIC; LIGHT; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OPEN READING FRAMES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; ZEAXANTHINS;

EID: 84917740741     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-014-3524-8     Document Type: Article

References (42)

1. Dall’Osto L, Lico C, Alric J, Giuliano G, Havaux M et al (2006) Lutein is needed for efficient chlorophyll triplet quenching in the major LHCII antenna complex of higher plants and effective photoprotection in vivo under strong light. BMC Plant Biol 6:32
2. Lichtenthaler HK (2007) Biosynthesis, accumulation and emission of carotenoids, α-tocopherol, plastoquinone, and isoprene in leaves under high photosynthetic irradiance. Photosynth Res 92:163–179
3. DellaPenna D, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annu Rev Plant Biol 57:711–738
4. Kim J, Smith JJ, Tian L, DellaPenna D (2009) The evolution and function of carotenoid hydroxylases in Arabidopsis. Plant Cell Physiol 50:463–479
5. Tian L, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D (2004) The Arabidopsis LUT1 locus encodes a member of the cytochrome P450 family that is required for carotenoid ε-ring hydroxylation activity. Proc Natl Acad Sci 101:402–407
6. Kim J, DellaPenna D (2006) Defining the primary route for lutein synthesis in plants: the role of Arabidopsis carotenoid β-ring hydroxylase CYP97A3. Proc Natl Acad Sci USA 103:3474–3479
7. Sun Z, Gantt E, Francis X Jr (1996) Cloning and functional analysis of the β-carotene hydroxylase of Arabidopsis thaliana. J Biol Chem 271:24349–24352
8. Tian L, DellaPenna D (2001) Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus. Plant Mol Biol 47:379–388
9. Davison PA, Hunter CN, Horton P (2002) Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature 418:203–206
10. Pogson BJ, Niyogi KK, Björkman O, DellaPenna D (1998) Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants. Proc Natl Acad Sci 95:13324–13329
11. Schwartz SH, Tan BC, Gage DA, Zeevaart JAD, McCarty DR (1997) Specific oxidative cleavage of carotenoids by VP14 of maize. Science 276:1872–1874
12. Castillo R, Fernández JA, Gómez-Gómez L (2005) Implications of carotenoid biosynthetic genes in apocarotenoid formation during the stigma development of Crocus sativus and its closer relatives. Plant Physiol 139:674–689
13. Linden H (1999) Carotenoid hydroxylase from Haematococcus pluvialis: cDNA sequence, regulation and functional complementation. Biochim Biophys Acta (BBA) Gene Struct Expr 1446:203–212
14. Takaichi S (2011) Carotenoids in algae: distributions, biosyntheses and functions. Mar Drugs 9:1101–1118
15. Wang Y, Chen T, Qin S (2012) Differential fatty acid profiles of Chlorella kessleri grown with organic materials. J Chem Technol Biotechnol. doi:10.1002/jctb.3881
16. Wang Y, Chen T, Qin S (2012) Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements. Biomass Bioenergy. doi:10.1016/j.biombioe.2012.09.018
17. Juarez AB, Barsanti L, Passarelli V, Evangelista V, Vesentini N et al (2008) In vivo microspectroscopy monitoring of chromium effects on the photosynthetic and photoreceptive apparatus of Eudorina unicocca and Chlorella kessleri. J Environ Monit 10:1313–1318
18. Romero DM, Ríos de Molina MC, Juárez AB (2011) Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri. Ecotoxicol Environ Saf 74:741–747
19. Li YC, Zhou WG, Hu B, Min M, Chen P, Ruan RR (2011) Integration of algae cultivation as biodiesel production feedstock with municipal wastewater treatment: strains screening and significance evaluation of environmental factors. Bioresour Technol 102:10861–10867
20. Rose TM, Henikoff JG, Henikoff S (2003) CODEHOP (COnsensus-DEgenerate hybrid oligonucleotide primer) PCR primer design. Nucleic Acids Res 31:3763–3766
21. Bjellqvist B, Hughes GJ, Pasquali C, Paquet N, Ravier F, Sanchez JC, Frutiger S, Hochstrasser D (1993) The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis 14:1023–1031
22. Cserzö M, Eisenhaber F, Eisenhaber B, Simon I (2002) On filtering false positive transmembrane protein predictions. Protein Eng 15:745–752
23. Emanuelsson O, Brunak S, Heijne GV, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2:953–971
24. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
25. Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704
26. Le SQ, Gascuel O (2008) An improved general amino acid replacement matrix. Mol Biol Evol 25:1307–1320
27. −ΔΔCT method. Methods 25:402–408
28. Sun N, Wang Y, Li YT, Huang JC, Chen F (2008) Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta). Process Biochem 43:1288–1292
29. Schmitt R, Fabry S, Kirk DL (1992) In search of molecular origins of cellular differentiation in Volvox and its relatives. Int Rev Cytol 139:189–265
30. Adl SM, Simpson AGB, Farmer MA, Andersen RA, Anderson OR et al (2005) The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J Eukaryot Microbiol 52:399–451
31. Schuler MA, Werck-Reichhart D (2003) Functional genomics of P450s. Annu Rev Plant Biol 54:629–667
32. Nelson D, Werck–eichhart D (2011) A P450-centric view of plant evolution. Plant J 66:194–211
33. Bak S, Beisson F, Bishop G, Hamberger B, Höfer R, Paquette S, Werck-Reichhart D (2011) Cytochromes P450. Arabidopsis Book 9:e0144
34. Woitsch S, Römer S (2003) Expression of xanthophyll biosynthetic genes during light-dependent chloroplast differentiation. Plant Physiol 132:1508–1517
35. Bohne F, Linden H (2002) Regulation of carotenoid biosynthesis genes in response to light in Chlamydomonas reinhardtii. Biochim Biophys Acta 1579:26–34
36. Steinbrenner J, Linden H (2001) Regulation of two carotenoid biosynthesis genes coding for phytoene synthase and carotenoid hydroxylase during stress-induced astaxanthin formation in the green alga Haematococcus pluvialis. Plant Physiol 125:810–817
37. Niyogi KK, Björkman O, Grossman AR (1997) The roles of specific xanthophylls in photoprotection. Proc Natl Acad Sci 94:14162–14167
38. Li Y, Huang J, Sandmann G, Chen F (2009) High-light and sodium chloride stress differentially regulate the biosynthesis of astaxanthin in Chlorella zofingiensis (Chlorophyceae). J Phycol 45:635–641
39. Katsuda T, Lababpour A, Shimahara K, Katoh S (2004) Astaxanthin production by Haematococcus pluvialis under illumination with LEDs. Enzyme Microb Technol 35:81–86
40. Lababpour A, Hada K, Shimahara K, Katsuda T, Katoh S (2004) Effects of nutrient supply methods and illumination with blue light emitting diodes (LEDs) on astaxanthin production by Haematococcus pluvialis. J Biosci Bioeng 98:452–456
41. Cunningham FX Jr, Pogson B, Sun ZR, McDonald KA, DellaPenna D, Gantt E (1996) Functional analysis of the β and ε-lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8:1613–1626
42. Cui HL, Wang YC, Qin S (2011) Molecular evolution of lycopene cyclases involved in the formation of carotenoids in eukaryotic algae. Plant Mol Biol Rep 29:1013–1020