Modeling an approach to define sensitivity of viral detection in sample matrices-examples with microarray readout

PDA Journal of Pharmaceutical Science and Technology

Volumn 68, Issue 6, 2014, Pages 589-594

  Feng, Szi Fei ^a   Jaing, Crystal ^b   Thissen, James B ^b   Wang, Christopher ^a   Duncan, Paul ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

Author keywords

Microarray; Sensitivity; Spike recovery; Virus detection

Indexed keywords

NUCLEASE; BIOLOGICAL PRODUCT; VIRUS DNA; VIRUS RNA;

ANALYTIC METHOD; CONFERENCE PAPER; HUMAN; HUMAN CELL; MICROARRAY ANALYSIS; MODEL; NONHUMAN; SENSITIVITY ANALYSIS; ULTRAFILTRATION; VIRUS DETECTION; DNA MICROARRAY; DRUG CONTAMINATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; PHARMACEUTICS; PREVENTION AND CONTROL; PROCEDURES; REPRODUCIBILITY; STANDARD; STANDARDS; TIME FACTOR; VIROLOGY; VIRUS; WORKFLOW;

BIOLOGICAL PRODUCTS; BIOPHARMACEUTICS; DNA, VIRAL; DRUG CONTAMINATION; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; TIME FACTORS; VIROLOGY; VIRUSES; WORKFLOW;

EID: 84915774663     PISSN: 10797440     EISSN: 19482124     Source Type: Journal    
DOI: 10.5731/pdajpst.2014.01011     Document Type: Conference Paper

References (6)

1. Erlandsson, L.; Rosenstierne, M. W.; McLoughlin, K.; Jaing, C.; Fomsgaard, A. The microbial detection array combined with random Phi29-amplification used as a diagnostic tool for virus detection in clinical samples. PLoS One 2011, 6 (8), e22631.
2. Victoria, J. G.; Wang, C.; Jones, M.; Jaing, C.; McLoughlin, K.; Gardner, S.; Delwart, E. L. Viral nucleic acids in live-attenuated vaccines: detection of minority variants and an adventitious virus. J. Virol. 2010, 84 (12), 6033-6040.
3. Jaing, C.; Gardner, S.; McLoughlin, K.; Thissen, J.; Slezak, T. Detection of adventitious viruses from biologicals using a broad spectrum microbial detection array. PDA J. Pharm. Sci. Technol. 2011, 65 (6), 668-674.
4. Gardner, S.; Jaing, C.; McLoughlin, K.; Slezak, T. A microbial detection array (MDA) for viral and bacterial detection. BMC Genomics 2010, 11 (1), 668.
5. Viennois, E.; Chen, F.; Laroui, H.; Baker, M. T.; Merlin, D. Dextran sodium sulfate inhibits the activities of both polymerase and reverse transcriptase: lithium chloride purification, a rapid and efficient technique to purify RNA. BMC Research Notes 2013, 6, 360.
6. Wang, C. J.; Feng, S. F.; Duncan, P. Defining a sample preparation workflow for advanced virus detection and understanding sensitivity by next-generation sequencing. PDA J. Pharm. Sci. Technol. 2014, 68 (6).