Comparison of whole blood RNA preservation tubes and novel generation RNA extraction kits for analysis of mRNA and miRNA profiles

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Häntzsch, Madlen ^a,b   Tolios, Alexander ^c   Beutner, Frank ^a,b   Nagel, Dorothea ^c   Thiery, Joachim ^a,b   Teupser, Daniel ^a,c   Holdt, Lesca M ^a,c  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b UNIVERSITY HOSPITAL LEIPZIG   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARGINASE 1; GELATINASE B; MESSENGER RNA; MICRORNA; MICRORNA 1; MICRORNA 133A; MICRORNA 30B; UNCLASSIFIED DRUG; DIAGNOSTIC KIT;

ACUTE HEART INFARCTION; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DEPLETION; DIAGNOSTIC KIT; GENE EXPRESSION; HUMAN; MAGMAX FOR STABILIZED BLOOD TUBES RNA ISOLATION KIT; NORGEN PRESERVED BLOOD RNA PURIFICATION KIT I; NORGEN PRESERVED BLOOD RNA PURIFICATION KIT II; PAXGENE BLOOD MIRNA KIT; QIASYMPHONY PAXGENE BLOOD RNA KIT; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION; BLOOD; BLOOD SAMPLING; BLOOD STORAGE; DEVICES; GENE EXPRESSION PROFILING; GENETICS; HEART INFARCTION; ISOLATION AND PURIFICATION; PROCEDURES; REPRODUCIBILITY;

BLOOD PRESERVATION; BLOOD SPECIMEN COLLECTION; GENE EXPRESSION PROFILING; HUMANS; MICRORNAS; MYOCARDIAL INFARCTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER;

EID: 84915733870     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0113298     Document Type: Article

References (32)

1. Rainen L, Oelmueller U, Jurgensen S, Wyrich R, Ballas C, et al. (2002) Stabilization of mRNA expression in whole blood samples. Clin Chem 48:1883-1890.
2. Baechler EC, Batliwalla FM, Karypis G, Gaffney PM, Moser K, et al. (2004) Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation. Genes and Immunity 5:347-353.
3. Asare AL, Kolchinsky SA, Gao Z, Wang R, Raddassi K, et al. (2008) Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation. BMC Genomics 9:474.
4. Menke A, Rex-Haffner M, Klengel T, Binder EB, Mehta D (2012) Peripheral blood gene expression: it all boils down to the RNA collection tubes. BMC Res Notes 5:1.
5. Tan SC, Yiap BC (2009) DNA, RNA, and protein extraction: the past and the present. J Biomed Biotechnol 2009:574398.
6. Duale N, Brunborg G, Ronningen KS, Briese T, Aarem J, et al. (2012) Human blood RNA stabilization in samples collected and transported for a large biobank. BMC Res Notes 5:510.
7. Jickling GC, Sharp FR (2011) Blood Biomarkers of Ischemic Stroke. Neurotherapeutics 8:349-360.
8. Vartanian K, Slottke R, Johnstone T, Casale A, Planck SR, et al. (2009) Gene expression profiling of whole blood: comparison of target preparation methods for accurate and reproducible microarray analysis. BMC Genomics 10:2.
9. Hsu J, Smith JD (2012) Genome-wide studies of gene expression relevant to coronary artery disease. Curr Opin Cardiol 27:210-213.
10. Malone JH, Oliver B (2011) Microarrays, deep sequencing and the true measure of the transcriptome. BMC Biol 9:34.
11. Wang Z, Gerstein M, Snyder M (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10:57-63.
12. Beyer M, Mallmann MR, Xue J, Staratschek-Jox A, Vorholt D, et al. (2012) High-resolution transcriptome of human macrophages. PLoS One 7:e45466.
13. Nagalakshmi U, Wang Z, Waern K, Shou C, Raha D, et al. (2008) The transcriptional landscape of the yeast genome defined by RNA sequencing. Science 320:1344-1349.
14. Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 5:621-628.
15. Jaguszewski M, Osipova J, Ghadri JR, Napp LC, Widera C, et al. (2014) A signature of circulating microRNAs differentiates takotsubo cardiomyopathy from acute myocardial infarction. Eur Heart J 35:999-1006.
16. Lorenzen JM, Kielstein JT, Hafer C, Gupta SK, Kumpers P, et al. (2011) Circulating miR-210 predicts survival in critically ill patients with acute kidney injury. Clin J Am Soc Nephrol 6:1540-1546.
17. Beutner F, Teupser D, Gielen S, Holdt LM, Scholz M, et al. (2011) Rationale and design of the Leipzig (LIFE) Heart Study: phenotyping and cardiovascular characteristics of patients with coronary artery disease. PLoS One 6:e29070.
18. Holdt LM, Hoffmann S, Sass K, Langenberger D, Scholz M, et al. (2013) Alu elements in ANRIL noncoding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS Genet 9:e1003588.
19. Harpster MH, Bandyopadhyay S, Thomas DP, Ivanov PS, Keele JA, et al. (2006) Earliest changes in the left ventricular transcriptome postmyocardial infarction. Mamm Genome 17:701-715.
20. Guzel S, Serin O, Guzel EC, Buyuk B, Yilmaz G, et al. (2013) Interleukin-33, matrix metalloproteinase-9, and tissue inhibitor [corrected] of matrix metalloproteinase-1 in myocardial infarction. Korean J Intern Med 28:165-173.
21. Garvin P, Nilsson L, Carstensen J, Jonasson L, Kristenson M (2008) Circulating Matrix Metalloproteinase-9 Is Associated with Cardiovascular Risk Factors in a Middle-Aged Normal Population. PLoS One 3:e1774.
22. Wang F, Long G, Zhao C, Li H, Chaugai S, et al. (2013) Plasma microRNA-133a is a new marker for both acute myocardial infarction and underlying coronary artery stenosis. J Transl Med 11:222.
23. Sayed AS, Xia K, Yang TL, Peng J (2013) Circulating microRNAs: a potential role in diagnosis and prognosis of acute myocardial infarction. Dis Markers 35:561-566.
24. Cheng C, Wang Q, You W, Chen M, Xia J (2014) MiRNAs as Biomarkers of Myocardial Infarction: A Meta-Analysis. PLoS One 9:e88566.
25. Devaux Y, Vausort M, McCann GP, Kelly D, Collignon O, et al. (2013) A Panel of 4 microRNAs Facilitates the Prediction of Left Ventricular Contractility after Acute Myocardial Infarction. PLoS One 8:e70644.
26. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45.
27. Assimes TL, Knowles JW, Basu A, Iribarren C, Southwick A, et al. (2008) Susceptibility locus for clinical and subclinical coronary artery disease at chromosome 9p21 in the multi-ethnic ADVANCE study. Hum Mol Genet 17:2320-2328.
28. Liu LF, Liang Z, Lv ZR, Liu XH, Bai J, et al. (2012) MicroRNA-15a/b are up-regulated in response to myocardial ischemia/reperfusion injury. J Geriatr Cardiol 9:28-32.
29. Ducharme A, Frantz S, Aikawa M, Rabkin E, Lindsey M, et al. (2000) Targeted deletion of matrix metalloproteinase-9 attenuates left ventricular enlargement and collagen accumulation after experimental myocardial infarction. J Clin Invest 106:55-62.
30. Matheson LA, Duong TT, Rosenberg AM, Yeung RSM (2008) Assessment of sample collection and storage methods for multicenter immunologic research in children. Journal of Immunological Methods 339:82-89.
31. Nikula T, Mykkanen J, Simell O, Lahesmaa R (2012) Genome-wide comparison of two RNA-stabilizing reagents for transcriptional profiling of peripheral blood. Transl Res.
32. Khan HA, Alhomida AS, Sobki SH, Moghairi AA, Koronki HE (2012) Blood cell counts and their correlation with creatine kinase and C-reactive protein in patients with acute myocardial infarction. Int J Clin Exp Med 5:50-55.