Human blood RNA stabilization in samples collected and transported for a large biobank

BMC Research Notes

Volumn 5, Issue , 2012, Pages

  Duale, Nur ^a   Brunborg, Gunnar ^a   Ronningen, Kjersti S ^a,d   Briese, Thomas ^b   Aarem, Jeanette ^a   Aas, Kaja K ^a   Magnus, Per ^a   Stoltenberg, Camilla ^a   Susser, Ezra ^b,c   Lipkin, Ian W ^b  

^a NORWEGIAN INSTITUTE OF PUBLIC HEALTH   (Norway)

^b Mailman School of Public Health   (United States)

^c NEW YORK STATE PSYCHIATRIC INSTITUTE   (United States)

^d OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Biobank; Blood RNA; Cord blood; Gene expression; PAXgene tubes; Quality control; RNA stabilization; Tempus tubes

Indexed keywords

RNA;

ARTICLE; BLOOD; COHORT ANALYSIS; FEMALE; HEALTH CARE FACILITY; HUMAN; LABORATORY DIAGNOSIS; PREGNANCY; QUALITY CONTROL;

COHORT STUDIES; FEMALE; HUMANS; PREGNANCY; QUALITY CONTROL; RNA; SPECIMEN HANDLING; TISSUE BANKS;

EID: 84866269022     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-5-510     Document Type: Article

References (18)

1. Cohort profile: the Norwegian Mother and Child Cohort Study (MoBa). Magnus P, Irgens LM, Haug K, Nystad W, Skjaerven R, Stoltenberg C, Int J Epidemiol 2006 35 1146 1150 10.1093/ije/dyl170 16926217 (Pubitemid 44605528)
2. The biobank of the Norwegian Mother and Child Cohort Study: a resource for the next 100years. Ronningen KS, Paltiel L, Meltzer HM, Nordhagen R, Lie KK, Hovengen R, et al. Eur J Epidemiol 2006 21 619 625 10.1007/s10654-006-9041-x 17031521 (Pubitemid 44546625)
3. Ex vivo induction of cytokine mRNA expression in human blood samples. Hartel C, Bein G, Muller-Steinhardt M, Kluter H, J Immunol Methods 2001 249 63 71 10.1016/S0022-1759(00)00334-3 11226464 (Pubitemid 32171959)
4. Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW, Biotechnol Lett 2006 28 1601 1613 10.1007/s10529-006-9127-2 16900335 (Pubitemid 44306128)
5. RNA integrity and the effect on the real-time qRT-PCR performance. Fleige S, Pfaffl MW, Mol Aspects Med 2006 27 126 139 10.1016/j.mam.2005.12.003 16469371
6. Assessment of two methods for handling blood in collection tubes with RNA stabilizing agent for surveillance of gene expression profiles with high density microarrays. Thach DC, Lin BC, Walter E, Kruzelock R, Rowley RK, Tibbetts C, et al. J Immunol Methods 2003 283 269 279 10.1016/j.jim.2003.10.004 14659918 (Pubitemid 37505016)
7. Stabilization of mRNA expression in whole blood samples. Rainen L, Oelmueller U, Jurgensen S, Wyrich R, Ballas C, Schram J, et al. Clin Chem 2002 48 1883 1890 12406972 (Pubitemid 35222745)
8. Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation. Baechler EC, Batliwalla FM, Karypis G, Gaffney PM, Moser K, Ortmann WA, et al. Genes Immun 2004 5 347 353 10.1038/sj.gene.6364098 15175644 (Pubitemid 39139761)
9. Molecular epidemiology biomarkers-sample collection and processing considerations. Holland NT, Pfleger L, Berger E, Ho A, Bastaki M, Toxicol Appl Pharmacol 2005 206 261 268 10.1016/j.taap.2004.10.024 15967217 (Pubitemid 40828246)
10. Biological sample collection and processing for molecular epidemiological studies. Holland NT, Smith MT, Eskenazi B, Bastaki M, Mutat Res 2003 543 217 234 10.1016/S1383-5742(02)00090-X 12787814 (Pubitemid 36645081)
11. Improvement of molecular monitoring of residual disease in leukemias by bedside RNA stabilization. Muller MC, Merx K, Weisser A, Kreil S, Lahaye T, Hehlmann R, et al. Leukemia 2002 16 2395 2399 10.1038/sj.leu.2402734 12454744 (Pubitemid 36054373)
12. Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks. Weber DG, Casjens S, Rozynek P, Lehnert M, Zilch-Schoneweis S, Bryk O, et al. Biomark Insights 2010 5 95 102 20981139
13. Assessment of a new RNA stabilizing reagent (Tempus Blood RNA) for minimal residual disease in onco-hematology using the EAC protocol. Prezeau N, Silvy M, Gabert J, Picard C, Leuk Res 2006 30 569 574 10.1016/j.leukres.2005.08. 027 16209886 (Pubitemid 43374586)
14. Assessment of sample collection and storage methods for multicenter immunologic research in children. Matheson LA, Duong TT, Rosenberg AM, Yeung RS, J Immunol Methods 2008 339 82 89 10.1016/j.jim.2008.08.003 18771669
15. Differential gene expression profiles are dependent upon method of peripheral blood collection and RNA isolation. Asare AL, Kolchinsky SA, Gao Z, Wang R, Raddassi K, Bourcier K, et al. BMC Genomics 2008 9 474 10.1186/1471-2164-9-474 18847473
16. RDML: structured language and reporting guidelines for real-time quantitative PCR data. Lefever S, Hellemans J, Pattyn F, Przybylski DR, Taylor C, Geurts R, et al. Nucleic Acids Res 2009 37 2065 2069 10.1093/nar/gkp056 19223324
17. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(Delta Delta C) method. Livak KJ, Schmittgen TD, Methods 2001 25 402 408 10.1006/meth.2001.1262 11846609 (Pubitemid 34164012)
18. Analyzing real-time PCR data by the comparative C(T) method. Schmittgen TD, Livak KJ, Nat Protoc 2008 3 1101 1108 10.1038/nprot.2008.73 18546601 (Pubitemid 351818697)