A high-throughput and cost-efficient DNA extraction protocol for the tree fruit crops of apple, sweet cherry, and peach relying on silica beads during tissue sampling

Molecular Breeding

Volumn 34, Issue 4, 2014, Pages 2225-2228

  Edge Garza, Daniel A ^a   Rowland, Terrence V ^a   Haendiges, Stacey ^a   Peace, Cameron ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

Marker assisted seedling selection; MASS; SBM; Silica bead method

Indexed keywords

CROPS; EXTRACTION; FRUITS; POLYMERASE CHAIN REACTION; SILICA GEL; THROUGHPUT;

DNA EXTRACTION; HIGH COSTS; HIGH-THROUGHPUT; MARKER-ASSISTED SEEDLING SELECTION; SBM; SEEDLING SELECTION; SILICA BEADS; SILICUM BEAD METHOD;

DNA;

MALUS X DOMESTICA; PRUNUS AVIUM; PRUNUS PERSICA;

EID: 84914811856     PISSN: 13803743     EISSN: 15729788     Source Type: Journal    
DOI: 10.1007/s11032-014-0160-x     Document Type: Article

References (18)

1. Angeles JGC, Laurena AC, Maetecson-Mendoza E (2005) Extraction of genomic DNA from the lipid-, polysaccharide-, and polyphenol-rich coconut (Cocosnucifera L.). Plant Mol Biol Rep 23:297a–297i
2. Cheng YJ, Guo WW, Yi HL, Pang XM, Deng X (2003) An efficient protocol for genomic DNA extraction from Citrus species. Plant Mol Biol Rep 21:177a–177g
3. Costa F, Van de Weg WE, Stella S, Dondini L, Pratesi D, Musacchi S, Sansavini S (2008) Map position and functional allelic diversity of Md-Exp7, a new putative expansin gene associated with fruit softening in apple (Malus × domestica Borkh.) and pear (Pyrus communis). Tree Genet Genomes 4:575–586
4. Demeke T, Jenkins GR (2010) Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits. Anal Bioanal Chem 396:1977–1990
5. Dilworth E, Frey JE (2000) A rapid method for high throughput DNA extraction from plant material for PCR amplification. Plant Mol Biol Rep 18:61–64
6. Edge-Garza DA, Peace CP, Zhu Y (2010) Enabling marker-assisted seedling selection in the Washington Apple Breeding Program. Acta Hortic 859:369–373
7. Evans K (2013) Apple breeding in the Pacific Northwest. Acta Hortic 976:75–78
8. Kim CS, Lee CH, Shin JS, Chung YS, Hyung NI (1997) A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP. Nucleic Acids Res 25:1085–1086
9. Lemke L, Rex M, Zyprian E, Öpfer RT (2011) A simple, inexpensive and environmentally friendly method for high throughput DNA extraction from grapevine (Vitis spp.). Vitis 50:7–10
10. Miller DN, Bryant JE, Madsen EL, Ghiorse WC (1999) Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples. Appl Environ Microbiol 65:4715–4724
11. Peace CP (2011) Fast-tracked MAB pipelining in peach: the endoPG genetic test for fruit texture type. Community Breeders’ Page, Quarterly Newsletter 2(3):4. http://www.rosbreed.org/sites/default/files/2011-08-31_RosBREED.Newsletter.pdf. Accessed 30 November 2013
12. Peace CP, Norelli J (2009) Genomics approaches to crop improvement in the Rosaceae. In: Folta K, Gardiner S (eds) Genetics and genomics of Rosaceae. Springer, New York, pp 19–53
13. Peace CP, Crisosto CH, Gradziel TM (2005) Endopolygalacturonase: a candidate gene for Freestone and Melting flesh in peach. Mol Breed 16:21–31
14. Randhawa HS, Mutti JS, Kidwell K, Morris CF, Chen X, Gill KS (2009) Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection. PLoS One 4(6):e5752
15. Santella RM (2006) Approaches to DNA/RNA extraction and whole genome amplification. Cancer Epidemiol Biomarkers Prev 15:1585–1587
16. Sebolt A (2011) Breeder profile: Nnadozie Oraguzie. Rosbreed Q Newsl 2(4):5–6. http://www.rosbreed.org/sites/default/files/2011-11-07_RosBREED.Newsletter.pdf. Accessed 24 November 2013
17. Slotta TAB, Brady L, Chao S (2008) High throughput tissue preparation for large-scale genotyping experiments. Mol Ecol Resour 8:83–87
18. Xin Z, Chen J (2012) A high throughput DNA extraction method with high yield and quality. Plant Methods 8:26