Monitoring antibacterial permeabilization in real time using time-resolved flow cytometry

Biochimica et Biophysica Acta - Biomembranes

Volumn 1848, Issue 2, 2015, Pages 554-560

  Freire, João Miguel ^a   Gaspar, Diana ^a   De La Torre, Beatriz Garcia ^b   Veiga, Ana Salomé ^a   Andreu, David ^b   Castanho, Miguel A R B ^a  

^a INSTITUTO DE MEDICINA MOLECULAR   (Portugal)

^b UNIVERSITAT POMPEU FABRA   (Spain)

Author keywords

Antimicrobial action; Antimicrobial peptide; Lipopolysaccharide; Live dead assay; Peptidelipid interaction; Time resolved flow cytometry

Indexed keywords

ESCHERICHIA COLI LIPOPOLYSACCHARIDE; PEPTIDE PEPR; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; ANTIMICROBIAL CATIONIC PEPTIDE; FLUORESCENT DYE; LIPOPOLYSACCHARIDE; ORGANIC COMPOUND; PROPIDIUM IODIDE; SYTO 9;

ARTICLE; BACTERIAL OUTER MEMBRANE; BACTERIAL STRAIN; BACTERICIDAL ACTIVITY; BACTERIUM CULTURE; BINDING KINETICS; CONTROLLED STUDY; DRUG DETERMINATION; DRUG EFFECT; DRUG MONITORING; DRUG PENETRATION; ESCHERICHIA COLI; FLOW CYTOMETRY; MEMBRANE DAMAGE; MEMBRANE PERMEABILITY; NONHUMAN; TIME RESOLVED FLOW CYTOMETRY; ZETA POTENTIAL; BIOASSAY; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CHEMISTRY; DRUG EFFECTS; KINETICS; MICROBIAL VIABILITY; PROCEDURES; SPECIES DIFFERENCE; SPECTROFLUOROMETRY; TIME;

ANTI-BACTERIAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BIOLOGICAL ASSAY; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENT DYES; KINETICS; LIPOPOLYSACCHARIDES; MICROBIAL VIABILITY; ORGANIC CHEMICALS; PROPIDIUM; SPECIES SPECIFICITY; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

EID: 84914125401     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2014.11.001     Document Type: Article

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