Delineating monoclonal antibody specificity by mass spectrometry

Journal of Proteomics

Volumn 114, Issue , 2015, Pages 115-124

  Korbakis, Dimitrios ^a,b   Prassas, Ioannis ^a,c   Brinc, Davor ^c   Batruch, Ihor ^c   Krastins, Bryan ^d   Lopez, Mary F ^d   Diamandis, Eleftherios P ^a,b,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY HEALTH NETWORK   (Canada)

^c MOUNT SINAI HOSPITAL   (Canada)

^d Thermo Fisher Scientific   (United States)

Author keywords

Immuno MS; Magnetic beads; Microtiter plates; Monoclonal antibodies; MSIA pipette tips; NHS activated beads

Indexed keywords

AGAROSE; ANTIGEN; MONOCLONAL ANTIBODY; POLYSTYRENE; PROTEOME; RIBONUCLEASE; AZGP1 PROTEIN, HUMAN; CARRIER PROTEIN; EPITOPE; GLYCOPROTEIN; PROSTATE SPECIFIC ANTIGEN;

ANIMAL CELL; ANTIBODY AFFINITY; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ANTIGEN SPECIFICITY; ARTICLE; BODY FLUID; FEMALE; MALE; MASS SPECTROMETRY; MICROPIPETTE; MOUSE; NONHUMAN; REPRODUCIBILITY; SEMINAL PLASMA; ANIMAL; BAGG ALBINO MOUSE; CHEMISTRY; IMMUNOLOGY; LIQUID CHROMATOGRAPHY; PROCEDURES; TANDEM MASS SPECTROMETRY;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CARRIER PROTEINS; CHROMATOGRAPHY, LIQUID; EPITOPES; FEMALE; GLYCOPROTEINS; MALE; MASS SPECTROMETRY; MICE; MICE, INBRED BALB C; PROSTATE-SPECIFIC ANTIGEN; TANDEM MASS SPECTROMETRY;

EID: 84913568715     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2014.11.004     Document Type: Article

References (31)

1. Anderson N.L. The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics 2002, 1:845-867.
2. Chiarella P., Fazio V.M. Mouse monoclonal antibodies in biological research: strategies for high-throughput production. Biotechnol Lett 2008, 30:1303-1310.
3. Ackermann B.L., Berna M.J. Coupling immunoaffinity techniques with MS for quantitative analysis of low-abundance protein biomarkers. Expert Rev Proteomics 2007, 4:175-186.
4. Madian A.G., Rochelle N.S., Regnier F.E. Mass-linked immuno-selective assays in targeted proteomics. Anal Chem 2013, 85:737-748.
5. Nelson R.W., Borges C.R. Mass spectrometric immunoassay revisited. J Am Soc Mass Spectrom 2011, 22:960-968.
6. Yassine H., Borges C.R., Schaab M.R., Billheimer D., Stump C., Reaven P., et al. Mass spectrometric immunoassay and MRM as targeted MS-based quantitative approaches in biomarker development: potential applications to cardiovascular disease and diabetes. Proteomics Clin Appl 2013, 7:528-540.
7. Ruigrok V.J., Levisson M., Eppink M.H., Smidt H., van der Oost J. Alternative affinity tools: more attractive than antibodies?. Biochem J 2011, 436:1-13.
8. Eveleigh J.W., Levy D.E. Immunochemical characteristics and preparative application of agarose-based immunosorbents. J. Solid-Phase. Biochem. 1977, 2:45-78.
9. Orthner C.L., Highsmith F.A., Tharakan J., Madurawe R.D., Morcol T., Velander W.H. Comparison of the performance of immunosorbents prepared by site-directed or random coupling of monoclonal antibodies. J Chromatogr 1991, 558:55-70.
10. Kaster J.A. Optimization of pressure-flow limits, strength, intraparticle transport and dynamic capacity by hydrogel solids content and bead size in cellulose immunosorbents. J Chromatogr 1993, 648:79-90.
11. Kennel S.J. Binding of monoclonal antibody to protein antigen in fluid phase or bound to solid supports. J Immunol Methods 1982, 55:1-12.
12. Yarmush M.L., Lu X.M., Yarmush D.M. Coupling of antibody-binding fragments to solid-phase supports: site-directed binding of F(ab')2 fragments. J Biochem Biophys Methods 1992, 25:285-297.
13. Subramanian A., Cotta K.E.V., Milbrath D.S., Velander W.H. Role of local antibody density effects on imunosorbent efficiency. J Chromatogr A 1994, 672:11-24.
14. Subramanian A., Velander W.H. Effect of antibody orientation on immunosorbent performance. J Mol Recognit 1996, 9:528-535.
15. Kulasingam V., Smith C.R., Batruch I., Buckler A., Jeffery D.A., Diamandis E.P. "Product ion monitoring" assay for prostate-specific antigen in serum using a linear ion-trap. J Proteome Res 2008, 7:640-647.
16. Kulasingam V., Smith C.R., Batruch I., Diamandis E.P. Immuno-mass spectrometry: quantification of low-abundance proteins in biological fluids. Methods Mol Biol 2011, 728:207-218.
17. Wang D., Hincapie M., Guergova-Kuras M., Kadas J., Takacs L., Karger B.L. Antigen identification and characterization of lung cancer specific monoclonal antibodies produced by mAb proteomics. J Proteome Res 2010, 9:1834-1842.
18. Sousa M.M., Steen K.W., Hagen L., Slupphaug G. Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis. Proteome Sci. 2011, 9:45.
19. Whiteaker J.R., Zhao L., Zhang H.Y., Feng L.C., Piening B.D., Anderson L., et al. Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers. Anal Biochem 2007, 362:44-54.
20. Lopez M.F., Rezai T., Sarracino D.A., Prakash A., Krastins B., Athanas M., et al. Selected reaction monitoring-mass spectrometric immunoassay responsive to parathyroid hormone and related variants. Clin Chem 2010, 56:281-290.
21. Drabovich A.P., Dimitromanolakis A., Saraon P., Soosaipillai A., Batruch I., Mullen B., et al. Differential diagnosis of azoospermia with proteomic biomarkers ECM1 and TEX101 quantified in seminal plasma. Sci Transl Med 2013, 5:212ra160.
22. Batruch I., Lecker I., Kagedan D., Smith C.R., Mullen B.J., Grober E., et al. Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J Proteome Res 2011, 10:941-953.
23. Batruch I., Smith C.R., Mullen B.J., Grober E., Lo K.C., Diamandis E.P., et al. Analysis of seminal plasma from patients with non-obstructive azoospermia and identification of candidate biomarkers of male infertility. J Proteome Res 2012, 11:1503-1511.
24. Drabovich A.P., Jarvi K., Diamandis E.P. Verification of male infertility biomarkers in seminal plasma by multiplex selected reaction monitoring assay. Mol Cell Proteomics 2011, 10. [M110 004127].
25. Shaw J.L., Diamandis E.P. Distribution of 15 human kallikreins in tissues and biological fluids. Clin Chem 2007, 53:1423-1432.
26. Yokoyama W.M., Christensen M., Santos G.D., Miller D. Production of monoclonal antibodies. Current protocols in immunology 2006, [Chapter 2:Unit 2 5]. J.E. Coligan (Ed.).
27. Cox J., Mann M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol 2008, 26:1367-1372.
28. Cox J., Matic I., Hilger M., Nagaraj N., Selbach M., Olsen J.V., et al. A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc 2009, 4:698-705.
29. Guergova-Kuras M., Kurucz I., Hempel W., Tardieu N., Kadas J., Malderez-Bloes C., et al. Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries. Mol Cell Proteomics 2011, 10. [M111 010298].
30. Zoller M., Matzku S. Antigen and antibody purification by immunoadsorption: elimination of non-biospecifically bound proteins. J Immunol Methods 1976, 11:287-295.
31. Ning Y., Wang Y., Li Y., Hong Y., Peng D., Liu Y., et al. An alternative strategy for high throughput generation and characterization of monoclonal antibodies against human plasma proteins using fractionated native proteins as immunogens. Proteomics 2006, 6:438-448.