Production, purification and characterization of a new cellulase from Bacillus subtilis that exhibit halophilic, alkalophilic and solvent-tolerant properties

Annals of Microbiology

Volumn 64, Issue 4, 2014, Pages 1839-1848

  Asha, Balachandrababu Malini ^a   Sakthivel, Natarajan ^a  

^a PONDICHERRY UNIVERSITY   (India)

Author keywords

Alkalophilic; Bacillus; Cellulase; Halophilic; Solvent tolerant

Indexed keywords

BACILLUS SUBTILIS;

EID: 84912047995     PISSN: 15904261     EISSN: 18692044     Source Type: Journal    
DOI: 10.1007/s13213-014-0835-x     Document Type: Article

References (35)

1. Annamalai N, Thavasi R, Vijayalakshmi S, Balasubramanian T (2011) A novel thermostable and halostable carboxymethylcellulase from marine bacterium Bacillus licheniformis AU01. World J Microbiol Biotechnol 27:2111–2115
2. Aona R, Ito M, Inoue A, Horikoshi K (1992) Isolation of toluene tolerant strain Pseudomonas aeruginosa. Biosci Biotechnol Biochem 56:145–146
3. Aygan A, Arikan B (2008) A new halo-alkaliphilic, thermostable endoglucanase from moderately halophilic Bacillus sp. C14 isolated from Van Soda Lake. Int J Agri Biol 10:369–374
4. Cho KM, Hong SJ, Math RK, Islam SM, Kim JO, Lee YH, Kim H, Yun HD (2008) Cloning of two cellulase genes from endophytic Paenibacillus polymyxa GS01 and comparison with cel 44C-man 26A. J Basic Microbiol 48:464–472
5. Dhiman TR, Zaman MS, Gimenez RR, Walters JL, Treacher R (2002) Performance of dairy cows fed forage treated with fibrolytic enzymes prior to feeding. Anim Feed Sci Technol 101:115–125
6. Donagy JA, Mckay AM (1993) Production and properties of an alkaline protease by Aureobasidium pullulans. J Appl Bacteriol 74:662–666
7. Doukyu N, Ogino H (2010) Organic solvent tolerant enzymes. Biochem Eng J 48:270–282
8. Grassin C, Fauquembergue P (1996) Fruit juices. In: Godfrey T, West S (eds) Industrial enzymology. Stockholm Press, New York, pp 225–264
9. Han SJ, Yoo YJ, Kang HS (1995) Characterization of bifunctional cellulase and its structural gene. The cell gene of Bacillus sp. D04 has exo and endoglucanase activity. J Biol Chem 270:26012–26019
10. Hebeish A, Ibrahim NA (2007) The impact of frontier sciences on textile industry. Colourage 54:41–55
11. Kambourova MN, Krilova R, Mandeva DA (2003) Purification and properties of a thermostable endoglucanase from Bacillus stearothermophilus MC7. J Mol Catal 22:307–313
12. Karmakar M, Ray RR (2011) Current trends in research and application of microbial cellulases. Res Microbiol 6:41–53
13. Kim JY, Hur SH, Hong JH (2005) Purification and characterization of alkaline cellulase from a newly isolated alkalophilic Bacillus sp. HSH-810. Biotechnol Lett 27:313–316
14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
15. Li X, Yu HY (2012) Purification and characterization of an organic-solvent-tolerant cellulase from a halotolerant isolate, Bacillus sp. L1. J Ind Microbiol Biotechnol 39:1117–1124
16. Lineweaver H, Burk D (1934) The determination of enzyme dissociation constant. J Am Chem Soc 56:658–666
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-Phenol reagent. J Biol Chem 193:265–275
18. Mansfield SD, Saddler JN, Gubitz GM (1998) Characterisation of endoglucanases from the brown rot fungi Gloeophyllum sepiarium and Gloeophyllum traberum. Enzym Microb Biotechnol 23:133–140
19. Marhuenda Egea FC, Bonete MJ (2002) Extreme halophilic enzymes in organic solvents. Curr Opin Biotechnol 13:385–389
20. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
21. Morissey JH (1981) Silver strain for proteins in polyacrylamide gels: a modified procedure with enhanced with uniform sensitivity. Anal Biochem 117:307–310
22. Murashima KT, Nishimura Y, Nakamura J, Kuga, Moriya T, Simuda N (2002) Purification and characterization of new endo-1, 4-β-D-glucanases from Rhisopus oryzae. Enzym Microb Biotechnol 30:319–326
23. Ogino H, Ishikawa H (2001) Enzymes which are stable in the presence of organic solvents. J Biosci Bioeng 91:109–1168
24. Percival ZYH, Himmel ME, Mielenz JR (2006) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24:452–481
25. Seneath PHA, Mair NS, Sharpe EM, Holt JG (1986) Bergeys manual of systematic bacteriology. Williams and Wilkins, Baltimore
26. Singh ARC, Kuhad, Ward OP (2007) In: Kuhad RC, Singh A (eds) Industrial application of microbial cellulases in lignocellulose biotechnology: future prospects. International Publishing House, New Delhi, pp 345–358
27. Sukumaran RK, Singhania RR, Pandey A (2005) Microbial cellulases-production, applications and challenges. J Sci Ind Res 64:832–844
28. Sunish Kumar R, Ayyadurai N, Pandiaraja P, Reddy AV, Venkateswarlu Y, Prakash O, Sakthivel N (2005) Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broad spectrum antifungal activity and biofertilizing traits. J Appl Microbiol 98:145–154
29. Ventosa A, Joaquin JN, Oren A (1998) Biology of moderately halophilic aerobic bacteria. Microbiol Mol Rev 62:504–544
30. Voget S, Steele HL, Streit WR (2006) Characterization of a metagenome-derived halotolerant cellulase. J Biotechnol 126:26–36
31. Wang CY, Hsieh YR, Ng CC, Chan H, Linc HT, Tzeng WS, Shyu YT (2009) Purification and characterization of a novel halostable cellulase from Salinivibrio sp. strain NTU-05. Enzym Microb Technol 44:373–379
32. Weisburg WG, Barns Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
33. Wood PJ (1980) Specificity in the interaction of direct dyes with polysaccharides. Carbohydr Res 85:271–287
34. Wu J, Ju LK (1998) Enhancing enzymatic saccharification of waste newsprint by sarfactant addition. Biotechnol Prog 14:649–652
35. Zaks A, Klibanov AM (1988) Enzymatic catalysis in nonaqueous solvents. J Biol Chem 263:3194–3201