Biochemical characterization of Yarrowia lipolytica LIP8, a secreted lipase with a cleavable C-terminal region

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1851, Issue 2, 2015, Pages 129-140

  Kamoun, Jannet ^a   Schué, Mathieu ^b   Messaoud, Wala ^a   Baignol, Justine ^b   Point, Vanessa ^c   Mateos Diaz, Eduardo ^c   Mansuelle, Pascal ^d   Gargouri, Youssef ^a   Parsiegla, Goetz ^c   Cavalier, Jean François ^c   Carrière, Frédéric ^c   Aloulou, Ahmed ^a,c  

^a UNIVERSITY OF SFAX   (Tunisia)

^b Bât A   (France)

^c Centre National de la Recherche Scientifique   (France)

^d AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Enzyme; Lipolysis; Pichia pastoris; Regioselectivity; Signal peptide; Yeast

Indexed keywords

AMINO ACID; FATTY ACID; FUNGAL ENZYME; GLYCEROL; LONG CHAIN TRIACYLGLYCEROL; MEDIUM CHAIN TRIACYLGLYCEROL; RECOMBINANT ENZYME; RECOMBINANT YLLIP8 ENZYME; SHORT CHAIN TRIACYLGLYCEROL; SIGNAL PEPTIDE; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; YARROWIA LIPOLYTICA LIP8; BILE ACID; FUNGAL PROTEIN; OLIVE OIL; RECOMBINANT PROTEIN; VEGETABLE OIL;

ACIDITY; ALKALINITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CULTURE MEDIUM; DRUG MANUFACTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME RELEASE; FATTY ACID TRANSPORT; FUNGAL STRAIN; HYDROLYSIS; LIPOLYSIS; MASS SPECTROMETRY; NONHUMAN; PH; PICHIA PASTORIS; PROTEIN CLEAVAGE; PROTEIN PROCESSING; STEREOCHEMISTRY; WESTERN BLOTTING; WILD TYPE; X RAY CRYSTALLOGRAPHY; YARROWIA LIPOLYTICA; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PICHIA; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION; YARROWIA;

PICHIA PASTORIS; YARROWIA LIPOLYTICA;

AMINO ACID SEQUENCE; BILE ACIDS AND SALTS; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; FATTY ACIDS, NONESTERIFIED; FUNGAL PROTEINS; GLYCEROL; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; LIPASE; LIPOLYSIS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PICHIA; PLANT OILS; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TRIGLYCERIDES; YARROWIA;

EID: 84911860569     PISSN: 13881981     EISSN: 18792618     Source Type: Journal    
DOI: 10.1016/j.bbalip.2014.10.012     Document Type: Article

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