Design, calibration and application of broad-range optical nanosensors for determining intracellular pH

Nature Protocols

Volumn 9, Issue 12, 2014, Pages 2841-2858

  Søndergaard, Rikke V ^a   Henriksen, Jonas R ^a   Andresen, Thomas L ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN; POLYACRYLAMIDE; ACRYLIC ACID RESIN; BAFILOMYCIN A1; CARBOXYLIC ACID; FLUORESCENT DYE; MACROLIDE; NANOPARTICLE; OREGON GREEN 488 CARBOXYLIC ACID; RHODAMINE;

ARTICLE; CALIBRATION; CELL LEVEL; CELL MEMBRANE; CONCENTRATION (PARAMETERS); CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENDOCYTOSIS; ENDOSOME; EQUIPMENT DESIGN; FLOW KINETICS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; IMAGE ANALYSIS; INTERMETHOD COMPARISON; INTERNALIZATION; INTRACELLULAR SPACE; INTRACELLULAR TRANSPORT; LYSOSOME; NANOSENSOR; NONHUMAN; PH; SENSITIVITY ANALYSIS; CHEMISTRY; DEVICES; DRUG EFFECTS; GENETIC PROCEDURES; HELA CELL LINE; IMAGE PROCESSING; NANOTECHNOLOGY; PROCEDURES;

ACRYLIC RESINS; BIOSENSING TECHNIQUES; CALIBRATION; CARBOXYLIC ACIDS; ENDOSOMES; EQUIPMENT DESIGN; FLUORESCEIN; FLUORESCENT DYES; HELA CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; IMAGE PROCESSING, COMPUTER-ASSISTED; LYSOSOMES; MACROLIDES; NANOPARTICLES; NANOTECHNOLOGY; RHODAMINES;

EID: 84911478902     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.196     Document Type: Article

References (46)

1. Casey, J.R., Grinstein, S. & Orlowski, J. Sensors and regulators of intracellular pH. Nat. Rev. Mol. Cell Biol. 11, 50-61 (2010).
2. Barry, M., Reynolds, J. & Eastman, A. Etoposide-induced apoptosis in human Hl-60 cells is associated with intracellular acidification. Cancer Res. 53, 2349-2357 (1993).
3. Korn ak, U. et al. Impaired glycosylation and cutis laxa caused by mutations in the vesicular H+-ATPase subunit ATP6V0A2. Nat. Genet. 40, 32-34 (2008).
4. Jahn, R., Lang, T. & Sudhof, T. Membrane fusion. Cell 112, 519-533 (2003).
5. Maxfield, F.R. & McGraw, T.E. Endocytic recycling. Nat. Rev. Mol. Cell Biol. 5, 121-132 (2004).
6. Hunte, C. et al. Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature 435, 1197-1202 (2005).
7. Andresen, T.L., Jensen, S.S. & Jorgensen, K. Advanced strategies in liposomal cancer therapy: problems and prospects of active and tumor-specific drug release. Prog. Lipid Res. 44, 68-97 (2005).
8. Bradley, M. et al. pH sensing in living cells using fluorescent microspheres. Bioorg. Med. Chem. Lett. 18, 313-317 (2008).
9. Lapresta-Fernandez, A. et al. Magnetic core-shell fluorescent pH ratiometric nanosensor using a Stober coating method. Anal. Chim. Acta 707, 164-170 (2011).
10. Hornig, S. et al. Biocompatible fluorescent nanoparticles for pH-sensoring. Soft Matter 4, 1169-1172 (2008).
11. Doussineau, T., Smaihi, M. & Mohr, G.J. Two-dye core/shell zeolite nanoparticles: a new tool for ratiometric pH measurements. Adv. Funct. Mater. 19, 117-122 (2009).
12. Benjaminsen, R.V. et al. Evaluating nanoparticle sensor design for intracellular pH measurements. ACS Nano 5, 5864-5873 (2011).
13. Sun, H., Almdal, K. & Andresen, T.L. Expanding the dynamic measurement range for polymeric nanoparticle pH sensors. Chem. Commun. 47, 5268-5270 (2011).
14. Kulichikhin, K.Y., Aitio, O., Chirkova, T.V. & Fagerstedt, K.V. Effect of oxygen concentration on intracellular pH, glucose-6-phosphate and NTP conte nt in rice (Oryza sativa) and wheat (Triticum aestivum) root tips: in vivo P-31-NMR study. Physiol. Plantarum 129, 507-518 (2007).
15. Sommer, S., Jahn, A., Funke, F. & Brenke, N. In vivo mea surements of the internal pH of Hediste (Nereis) diversicolor (Annelida, Polychaeta) exposed to ambient sulphidic conditions using pH microelectrodes. Naturwissenschaften 87, 283-287 (2000).
16. Kneipp, J., Kneipp, H., Wittig, B. & Kneipp, K. Novel optical nanosensors for probing and imaging live cells. Nanomedicine 6, 214-226 (2010).
17. Han, J. & Burgess, K. Fluorescent indicators for intracellular pH. Chem. Rev. 110, 2709-2728 (2010).
18. Li, X., Gao, X., Shi, W. & Ma, H. Design strategies for water-soluble small molecular chromogenic and fluorogenic probes. Chem. Rev. 114, 590-659 (2014).
19. Myochin, T. et al. Rational design of ratiometric near-infrared fluorescent pH probes with various pKa values, based on aminocyanine. J. Am. Chem. Soc. 133, 3401-3409 (2011).
20. Gan B.S.,Krump E.,Shrode L.D.,Grinstein S Loading pyranine via purinergic receptors or hypotonic stress for measurement of cytosolic pH by imaging Am. J. Physiol. 275, C1158-C1166, 1998).
21. Bizzarri, R., Serresi, M., Luin, S. & Beltram, F. Green fluorescent protein based pH indicators for in vivo use: a review. Anal. Bioanal. Chem. 393, 1107-1122 (2009).
22. Clark, H.A., Kopelman, R., Tjalkens, R. & Philbert, M.A. Optical nanosensors for chemical analysis inside single living cells. 2. Sensors for pH and calcium and the intracellular application of PEBBLE sensors. Anal. Chem. 71, 4837-4843 (1999).
23. Dennis, A.M., Rhee, W.J., Sotto, D., Dublin, S.N. & Bao, G. Quantum dot-fluorescent protein FRET probes for sensing intracellular pH. ACS Nano 6, 2917-2924 (2012).
24. Modi, S. et al. A DNA nanomachine that maps spatial and temporal pH changes inside living cells. Nat. Nanotechnol. 4, 325-330 (2009).
25. Mellman, I., Fuchs, R. & Helenius, A. Acidification of the endocytic and exocytic pathways. Annu. Rev. Biochem. 55, 663-700 (1986).
26. Luzio, J.P., Pryor, P.R. & Bright, N.A. Lysosomes: fusion and function. Nat. Rev. Mol. Cell Biol. 8, 622-632 (2007).
27. Su, M.H. et al. 1,9-Dihydro-3-phenyl-4H-pyrazolo[3,4-b]quinolin-4-one, a novel fluorescent probe for extreme pH measurement. Chem. Commun. 11, 960-961 (2001).
28. Schulz, A. et al. Evaluation of fluorescent polysaccharide nanoparticles for pH-sensing. Org. Biomol. Chem. 7, 1884-1889 (2009).
29. Lee, R.J., Wang, S. & Low, P.S. Measurement of endosome pH following folate receptor-mediated endocytosis. Biochim. Biophys. Acta 1312, 237-242 (1996).
30. Sun, H., Benjaminsen, R.V., Almdal, K. & Andresen, T.L. Hyaluronic acid immobilized polyacrylamide nanoparticle sensors for CD44 receptor targeting and pH measurement in cells. Bioconjug. Chem. 23, 2247-2255 (2012).
31. Coupland, P.G., Briddon, S.J. & Aylott, J.W. Using fluorescent pH-sensitive nanosensors to report their intracellular location after Tat-mediated delivery. Integr. Biol. 1, 318-323 (2009).
32. Disbrow, G.L., Hanover, J.A. & Schlegel, R. Endoplasmic reticulum-localized human papillomavirus type 16 E5 protein alters endosomal pH but not trans-Golgi pH. J. Virol. 79, 5839-5846 (2005).
33. Jankowski, A. et al. In situ measurements of the pH of mammalian peroxisomes using the fluorescent protein pHluorin. J. Biol. Chem. 276, 48748-48753 (2001).
34. Ll opis, J., McCaffery, J.M., Miyawaki, A., Farquhar, M.G. & Tsien, R.Y. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95, 6803-68 08 (1998).
35. Yue, Z. et al. Surface charge affects cellular uptake and intracellular trafficking of chitosan-based nanoparticles. Biomacromolecules 12, 2440-2446 (2011).
36. Benjaminsen, R.V., Mattebjerg, M.A., Henriksen, J.R., Moghimi, S.M. & Andresen, T.L. The possible " proton sponge" effect of polyethylenimine (PEI) does not include change in lysosomal pH. Mol. Ther. 21, 149-157 (2013).
37. Doussineau, T., Trupp, S. & Mohr, G.J. Ratiometric pH-nanosensors based on rhodamine-doped silica nanoparticles functionalized with a naphthalimide derivative. J. Colloid Interface Sci. 339, 266-270 (2009).
38. Borisov, S.M., Herrod, D.L. & Klimant, I. Fluorescent poly(styrene-block-vinylpyrrolidone) nanobeads for optical sensing of pH. Sens. Actuators B 139, 52-58 (2009).
39. McNama ra, K.P. et al. Synthesis, characterization, and application of fluorescence sensing lipobeads for intracellular pH measurements. Anal. Chem. 73, 3240-3246 (2001).
40. Aylott, J. Optical nanosensors : an enabling technology for intracellular measurements. Analyst 128, 309-312 (2003).
41. Buck S.M.,et al Optochemical nanosensor PEBBLEs: photonic explorers for bioanalysis with biologically localized embedding. Curr. Opin. Chem. Biol. 8, 540-546, 2004).
42. Sun, H., Andresen, T.L., Benjaminsen, R.V. & Almdal, K. Polymeric nanosensors for measuring the full dynamic pH range of endosomes and ly sosomes in mammalian cells. J. Biomed. Nanotechnol. 5, 676-682 (2009).
43. Domingos, R.F. et al. Characterizing manufactured nanoparticles in the environment: multimethod determination of particle sizes. Environ. Sci. Technol. 43, 7277-7284 (2009).
44. Peng, J. et al. Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparticle sensors. Anal. Bioanal. Chem. 388, 645-654 (2007).
45. Stone, V., Johnston, H. & Schins, R.P. Development of in vitro systems for nanotoxicology: methodological considerations. Crit. Rev. Toxicol. 39, 613-626 (2009).
46. Kroll, A., Pillukat, M.H., Hahn, D. & Schnekenburger, J. Current in vitro methods in nanoparticle risk assessment: limitations and challenges. Eur. J. Pharm. Biopharm. 72, 370-377 (2009).