Evaluation of the Xpert vanA/vanB assay using enriched inoculated broths for direct detection of vanB vancomycin-resistant enterococci

Journal of Clinical Microbiology

Volumn 52, Issue 12, 2014, Pages 4293-4297

  Zhou, X ^a   Arends, J P ^a   Kampinga, G A ^a   Ahmad, H M ^a   Dijkhuizen, B ^a   Van Barneveld, P ^a   Rossen, J W A ^a   Friedrich, A W ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BROTH DILUTION; CEPHEID XPERT VANA ASSAY; CEPHEID XPERT VANB ASSAY; CONTROLLED STUDY; FALSE POSITIVE RESULT; GENE IDENTIFICATION; HUMAN; INTESTINE FLORA; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PROCESS OPTIMIZATION; SENSITIVITY AND SPECIFICITY; VANB GENE; VANCOMYCIN RESISTANT ENTEROCOCCUS; EVALUATION STUDY; GENETICS; GRAM-POSITIVE BACTERIAL INFECTIONS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PROCEDURES; RECTUM;

BACTERIAL PROTEIN; VANB PROTEIN, ENTEROCOCCUS;

BACTERIAL PROTEINS; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PREDICTIVE VALUE OF TESTS; RECTUM; SENSITIVITY AND SPECIFICITY; VANCOMYCIN-RESISTANT ENTEROCOCCI;

EID: 84910118990     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01125-14     Document Type: Article

References (21)

1. Leclercq R. 2009. Epidemiological and resistance issues in multidrug-resistant staphylococci and enterococci. Clin. Microbiol. Infect. 15:224-231. http://dx.doi.org/10.1111/j.1469-0691.2009.02739.x.
2. Courvalin P. 2006. Vancomycin resistance in gram-positive cocci. Clin. Infect. Dis. 42(Suppl 1):S25-S34. http://dx.doi.org/10.1086/491711.
3. Birgand G, Ruimy R, Schwarzinger M, Lolom I, Bendjelloul G, Houhou N, Armand-Lefevre L, Andremont A, Yazdanpanah Y, Lucet JC. 2013. Rapid detection of glycopeptide-resistant enterococci: impact on decision-making and costs. Antimicrob. Resist. Infect. Control. 2:30. http://dx.doi.org/10.1186/2047-2994-2-30.
4. Bourdon N, Berenger R, Lepoultier R, Mouet A, Lesteven C, Borgey F, Fines-Guyon M, Leclercq R, Cattoir V. 2010. Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay. Diagn. Microbiol. Infect. Dis. 67:291-293. http://dx.doi.org/10.1016/j.diagmicrobio.2010.02.009.
5. Marner ES, Wolk DM, Carr J, Hewitt C, Dominguez LL, Kovacs T, Johnson DR, Hayden RT. 2011. Diagnostic accuracy of the Cepheid GeneXpert vanA/vanB assay ver. 1.0 to detect the vanA and vanB vancomycin resistance genes in Enterococcus from perianal specimens. Diagn. Microbiol. Infect. Dis. 69:382-389. http://dx.doi.org/10.1016/j.diagmicrobio.2010.11.005.
6. Zabicka D, Strzelecki J, Wozniak A, Strzelecki P, Sadowy E, Kuch A, Hryniewicz W. 2012. Efficiency of the Cepheid Xpert vanA/vanB assay for screening of colonization with vancomycin-resistant enterococci during hospital outbreak. Antonie Van Leeuwenhoek 101:671-675. http://dx.doi.org/10.1007/s10482-011-9681-z.
7. Stamper PD, Cai M, Lema C, Eskey K, Carroll KC. 2007. Comparison of the BD GeneOhm VanR assay to culture for identification of vancomycin-resistant enterococci in rectal and stool specimens. J. Clin. Microbiol. 45:3360-3365. http://dx.doi.org/10.1128/JCM.01458-07.
8. Ballard SA, Grabsch EA, Johnson PD, Grayson ML. 2005. Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci: interference by vanB-containing anaerobic bacilli. Antimicrob. Agents Chemother 49:77-81. http://dx.doi.org/10.1128/AAC.49.1.77-81.2005.
9. Ballard SA, Pertile KK, Lim M, Johnson PD, Grayson ML. 2005. Molecular characterization of vanB elements in naturally occurring gut anaerobes. Antimicrob. Agents Chemother 49:1688-1694. http://dx.doi.org/10.1128/AAC.49.5.1688-1694.2005.
10. Graham M, Ballard SA, Grabsch EA, Johnson PD, Grayson ML. 2008. High rates of fecal carriage of nonenterococcal vanB in both children and adults. Antimicrob. Agents Chemother 52:1195-1197. http://dx.doi.org/10.1128/AAC.00531-07.
11. Stinear TP, Olden DC, Johnson PD, Davies JK, Grayson ML. 2001. Enterococcal vanB resistance locus in anaerobic bacteria in human faeces. Lancet 357:855-856. http://dx.doi.org/10.1016/S0140-6736(00)04206-9.
12. Werner G, Klare I, Fleige C, Geringer U, Witte W, Just HM, Ziegler R. 2012. Vancomycin-resistant vanB-type Enterococcus faecium isolates expressing varying levels of vancomycin resistance and being highly prevalent among neonatal patients in a single ICU. Antimicrob. Resist. Infect. Control 1:21. http://dx.doi.org/10.1186/2047-2994-1-21.
13. Hegstad K, Giske CG, Haldorsen B, Matuschek E, Schonning K, Leegaard TM, Kahlmeter G, Sundsfjord A, Nordic AST VRE Detection Study Group. 2014. Performance of the EUCAST disk diffusion method, the CLSI agar screen method, and the Vitek 2 automated antimicrobial susceptibility testing system for detection of clinical isolates of enterococci with low-and medium-level VanB-type vancomycin resistance: a multicenter study. J. Clin. Microbiol. 52:1582-1589. http://dx.doi.org/10.1128/JCM.03544-13.
14. Arends JP, Zhou X, Kampinga GA, Meessen NEL, Friedrich AW. 2012. Prevalence of phenotypically vancomycin susceptible, but vanB-PCR positive, Enterococcus faecium: do we overlook VRE vanB carrying strains in our hospital? Poster P1289. Abstr 22nd Eur Congr Clin Microbiol Infect Dis. ESCMID. London, United Kingdom.
15. Coombs GW, Pearson JC, Daley DA, Le T, Robinson OJ, Gottlieb T, Howden BP, Johnson PD, Bennett CM, Stinear TP, Turnidge JD, Australian Group on Antimicrobial Resistance. 2014. Molecular epidemiology of enterococcal bacteremia in Australia. J. Clin. Microbiol 52: 897-905. http://dx.doi.org/10.1128/JCM.03286-13.
16. Howden BP, Holt KE, Lam MM, Seemann T, Ballard S, Coombs GW, Tong SY, Grayson ML, Johnson PD, Stinear TP. 2013. Genomic insights to control the emergence of vancomycin-resistant enterococci. mBio 4:e00412-13. http://dx.doi.org/10.1128/mBio.00412-13.
17. European Committee on Antimicrobial Susceptibility Testing. 2014. Breakpoint tables for interpretation of MICs and zone diameters. European Committee on Antimicrobial Susceptibility Testing, Växjö, Sweden. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST-files/Breakpoint-tables/Breakpoint-table-v-4.0.pdf.
18. D'Agata EM, Gautam S, Green WK, Tang YW. 2002. High rate of false-negative results of the rectal swab culture method in detection of gastrointestinal colonization with vancomycin-resistant enterococci. Clin. Infect. Dis. 34:167-172. http://dx.doi.org/10.1086/338234.
19. Satake S, Clark N, Rimland D, Nolte FS, Tenover FC. 1997. Detection of vancomycin-resistant enterococci in fecal samples by PCR. J. Clin. Microbiol. 35:2325-2330.
20. Wilson EB. 1927. Probable inference, the law of succession, and statistical inference. J. Am. Stat. Assoc. 22:209-212. http://dx.doi.org/10.1080/01621459.1927.10502953.
21. Palladino S, Kay ID, Flexman JP, Boehm I, Costa AM, Lambert EJ, Christiansen KJ. 2003. Rapid detection of vanA and vanB genes directly from clinical specimens and enrichment broths by real-time multiplex PCR assay. J. Clin. Microbiol. 41:2483-2486. http://dx.doi.org/10.1128/JCM.41.6.2483-2486.2003.