Application of real-time PCR for the detection of Strongyloides spp. in clinical samples in a reference center in Spain

Acta Tropica

Volumn 142, Issue , 2015, Pages 20-25

  Saugar, José M ^a   Merino, Francisco J ^b   Martín Rabadán, Pablo ^c   Fernández Soto, Pedro ^d   Ortega, Sheila ^a   Gárate, Teresa ^a   Rodríguez, Esperanza ^a  

^a INSTITUTO DE SALUD CARLOS III   (Spain)

^b HOSPITAL SEVERO OCHOA   (Spain)

^c HOSPITAL GENERAL UNIVERSITARIO GREGORIO MARAÑÓN   (Spain)

^d UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Diagnosis; Melting curve analysis; Real time PCR; Spain; Strongyloides spp.; Strongyloidiasis

Indexed keywords

DNA 18S; HELMINTH DNA; RNA 18S;

DETECTION METHOD; ENDEMIC SPECIES; GENETIC ANALYSIS; HEALTH RISK; INFECTIVITY; PARASITIC DISEASE; PARASITOLOGY; POLYMERASE CHAIN REACTION;

ADOLESCENT; ADULT; AGED; ARTICLE; CHILD; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; FECES ANALYSIS; FEMALE; GENE TARGETING; GENETIC SIMILARITY; GEOGRAPHIC DISTRIBUTION; HUMAN; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MALE; MOLECULAR DIAGNOSIS; MULTICENTER STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; PARASITE LOAD; PARASITE PREVALENCE; PRESCHOOL CHILD; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE; SCREENING TEST; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; STRONGYLOIDES; STRONGYLOIDES FUELLEBORNI; STRONGYLOIDES STERCORALIS; STRONGYLOIDIASIS; VALIDATION STUDY; VERY ELDERLY; ANIMAL; EVALUATION STUDY; FECES; GENETICS; HELMINTHIASIS; IMMUNOCOMPROMIZED PATIENT; INTESTINAL DISEASES, PARASITIC; ISOLATION AND PURIFICATION; MIDDLE AGED; MOLECULAR GENETICS; PARASITOLOGY; PROCEDURES; SPAIN; YOUNG ADULT;

MADRID [SPAIN]; SPAIN;

STRONGYLOIDES;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANIMALS; BASE SEQUENCE; CHILD; CHILD, PRESCHOOL; FECES; FEMALE; HELMINTHIASIS; HUMANS; IMMUNOCOMPROMISED HOST; INTESTINAL DISEASES, PARASITIC; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SPAIN; STRONGYLOIDES; STRONGYLOIDES STERCORALIS; STRONGYLOIDIASIS; YOUNG ADULT;

EID: 84910020978     PISSN: 0001706X     EISSN: 18736254     Source Type: Journal    
DOI: 10.1016/j.actatropica.2014.10.020     Document Type: Article

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