High salt buffer improves integrity of RNA after fluorescence-activated cell sorting of intracellular labeled cells

Journal of Biotechnology

Volumn 192, Issue Part A, 2014, Pages 62-65

  Nilsson, Helén ^a   Krawczyk, Krzysztof M ^a   Johansson , Martin E ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Fixation; Fluorescence activated cell sorting; Intracellular antibody labeling; NaCl; RNA integrity

Indexed keywords

CELL CULTURE; CELL PROLIFERATION; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; NITROGEN FIXATION; NUCLEIC ACIDS; SODIUM CHLORIDE; SORTING;

BIOLOGICAL PROCESS; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION DATA; GENE EXPRESSION PROFILES; HETEROGENEOUS POPULATIONS; INTRACELLULAR ANTIBODIES; NACL; PERMEABILIZATION;

RNA;

BUFFER; RNA; SODIUM CHLORIDE; ALEXA FLUOR 647; ANTIBODY; CARBOCYANINE; CYTOKERATIN 7; CYTOKERATIN 8; FLUORESCENT DYE; FORMALDEHYDE; METHANOL; PARAFORMALDEHYDE; POLYMER;

ARTICLE; CELL LABELING; CELL MEMBRANE PERMEABILITY; CELL POPULATION; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; RNA DEGRADATION; RNA ISOLATION; CELL LINE; DRUG EFFECTS; FLOW CYTOMETRY; IMMUNOLOGY; ISOLATION AND PURIFICATION; PROCEDURES; RNA STABILITY; STAINING; TUMOR CELL LINE;

ANTIBODIES; BUFFERS; CARBOCYANINES; CELL LINE; CELL LINE, TUMOR; FLOW CYTOMETRY; FLUORESCENT DYES; FORMALDEHYDE; HUMANS; KERATIN-7; KERATIN-8; METHANOL; POLYMERS; RNA; RNA STABILITY; SODIUM CHLORIDE; STAINING AND LABELING;

EID: 84909635513     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.09.016     Document Type: Article

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